BDMC protects AD in vitro via AMPK and SIRT1

Abstract

BackgroundAlzheimer’s disease (AD) is a common neurodegenerative disorder without any satisfactory therapeutic approaches. AD is mainly characterized by the deposition of β-amyloid protein (Aβ) and extensive neuronal cell death. Curcumin, with anti-oxidative stress (OS) and cell apoptosis properties, plays essential roles in AD. However, whether bisdemethoxycurcumin (BDMC), a derivative of curcumin, can exert a neuroprotective effect in AD remains to be elucidated.MethodsIn this study, SK-N-SH cells were used to establish an in vitro model to investigate the effects of BDMC on the Aβ1–42-induced neurotoxicity. SK-N-SH cells were pretreated with BDMC and with or without compound C and EX527 for 30 min after co-incubation with rotenone for 24 h. Subsequently, western blotting, cell viability assay and SOD and GSH activity measurement were performed.ResultsBDMC increased the cell survival, anti-OS ability, AMPK phosphorylation levels and SIRT1 in SK-N-SH cells treated with Aβ1–42. However, after treatment with compound C, an AMPK inhibitor, and EX527, an SIRT1inhibitor, the neuroprotective roles of BDMC on SK-N-SH cells treated with Aβ1–42 were inhibited.ConclusionThese results suggest that BDMC exerts a neuroprotective role on SK-N-SH cells in vitro via AMPK/SIRT1 signaling, laying the foundation for the application of BDMC in the treatment of neurodegenerative diseases related to AMPK/SIRT1 signaling.

Auditory or Audiovisual Stimulation Ameliorates Cognitive Impairment and Neuropathology in ApoE4 Knock-In Mice

“…Moreover, the pathological processes triggered by Aβ deposition promote further deposition, leading to a response cascade. It has also been reported that Aβ is neurotoxic, thus inducing nerve cell apoptosis and, in turn, impairing cognitive function [ 46 , 47 ]. Abnormalities in the cholinergic system in AD also induce abnormal amyloid precursor protein metabolism and tau phosphorylation, resulting in neurotoxicity, neuroinflammation, and apoptosis [ 48 , 49 ].…”

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“…no. HY-15452; MedChemExpress) at 37˚C, followed by LPS induction and propofol treatment ( 18 , 19 ). Untreated H9C2 cells were regarded as the control group.…”

rTMS alleviates AD-induced cognitive impairment by inhibitng apoptosis in SAMP8 mouse

“…According to the manufacturer’s instructions, the total RNA of the mouse cortex and hippocampus was isolated with TRIzol reagent, and then cDNA was obtained by reverse transcription. qRT-PCR was performed as previously described [ 33 ]. Calculations were measured using the 2 −ΔΔCt method to analyze the relative expression of each gene, and the bar chart was drawn using GraphPad Prism 5.0.…”