Be careful with lentils! About a forensic observation

Oertel, Laetitia; Gressel, Anne; Tortel, M.-C.; Cannet, Catherine; Berthelon, Laurent; Abou-Bacar, Ahmed; Raul, Jean‐Sébastien; Farrugia, Audrey

doi:10.1007/s00414-020-02389-8

Cited by 2 publications

(4 citation statements)

References 8 publications

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“…Usually, it also shows typical eggs in the uterus and the characteristic narrow meromyarian layers (two to three muscle layers per quarter section divided by four cords). [16] In conclusion, in case of any doubt about the parasite etiology, the clinical specimens have to be systematically addressed to the parasitology laboratory in parallel before the tissue fixation which is mandatory for histological examination, and in order to get a specialized advice on a fresh sample.…”

Section: Discussionmentioning

confidence: 99%

Some undesirable traps which can mislead the pathologist

et al. 2021

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In clinical laboratories, the diagnosis of parasite diseases can sometimes be challenging for non-expert microbiologists. Indeed, in spite of the advent of the molecular biology, macroscopic and microscopic examinations still remain essential. Nonetheless, it is usually not automated and requires great skills to complete the correct diagnosis. It is not infrequent that inert elements mislead to erroneous diagnoses. Through three different concrete examples, this article aims at underscoring the actual risk of parasite misidentification and at highlighting the systematic approach to be conducted in order to enable reliable diagnosis.

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Section: Discussionmentioning

confidence: 99%

Some undesirable traps which can mislead the pathologist

et al. 2021

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“…Oertel et al (2021) reported a forensic observation of a multi‐visceral dispersion of exogenous particles, round to oval bodies containing a lobulated and optically empty internal part, surrounded by an eosinophilic membrane, evoking two hypotheses: aspired vegetable particles or parasitic infection, like pinworm larva. In order to affirm or invalidate parasitological hypothesis, a PCR analysis was performed on DNA extracted directly from gastric fluid, and the DNA sequencing of specific product showed a Fabaceae plant family, Lens culinaris .…”

Section: Discussionmentioning

confidence: 99%

“…The hyaline rings observed in case of hookworm eggs are the same in PG. It was already reported that hyaline rings could be mistaken for others infections, as Coccidioides (Pereira, Prichard, Khalid, Medich, & Silverman, 2001), Strongyloides stercoralis infestation (Kumar, Narasimha, Prasad, & Deo, 2011), and pinworm larvae (Oertel et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…The authors applied on diagnostic methods such as PCR, immunohistochemical studies and in situ hybridization to identify the parasite. Once identified through morphology, additional tests were performed to identify the exact pathogen, as the case presented typical microscopic characteristics of malignancy Oertel et al (2021). reported a forensic observation of a multivisceral dispersion of exogenous particles, round to oval bodies containing a lobulated and optically empty internal part, surrounded by an eosinophilic membrane, evoking two hypotheses: aspired vegetable particles or parasitic infection, like pinworm larva.…”

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Comparative evaluation of vegetable matter involved lesions with oral parasitic infections in the oral cavity

Pinheiro

Leite

Silva

et al. 2021

Microscopy Res & Technique

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The current research aims to perform a comparative evaluation of vegetable matter involved lesions with oral parasitic infections found in oral mucosa, presenting histochemical methods to differentiate their microscopic similarities. Eight cases were selected out of a sample of 1.975 reports from a single Oral and Maxillofacial Pathology Service of the author's institution from 2012 to 2019. Specimens were examined by hematoxylin and eosin staining (HE), periodic acid‐Schiff (PAS) staining, Gomori–Grocott staining, Ziehl–Neelsen staining, Giemsa, and mucicarmine staining. Microscopic analysis included fluorescence, polarized light, and confocal microscopy. Microscopically, in HE coloration, hookworm eggs showed as eosinophilic. Inflammatory multinucleated giant cells and lymphocytes, were usually related to the nematode eggs, forming an intense inflammatory infiltrate. Biofluorescent properties of eggs and larvae revealed to be sensitive in the detection of parasitic structures contrasting with the inflamed connective tissue. Vegetable presence was confirmed by polarized light microscopy and it was found to be associated with microbial biofilms. Confocal microscopy has showed to be an excellent method for morphotype differentiation of parasitic eggs. Parasitic infection and vegetable matter displayed similarities in the inflammatory response, but the latter can rot and agglomerate biofilms. The microscopic diagnosis of such infections requires the interpretation of challenging morphological features since the parasites are usually sectioned and mixed with an inflammatory reaction. These histochemical approaches proved to be excellent to distinguish both lesions.

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Be careful with lentils! About a forensic observation

Cited by 2 publications

References 8 publications

Some undesirable traps which can mislead the pathologist

Some undesirable traps which can mislead the pathologist

Comparative evaluation of vegetable matter involved lesions with oral parasitic infections in the oral cavity

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