Beads-Based Electrochemical Assay for the Detection of Influenza Hemagglutinin Labeled with CdTe Quantum Dots

Abstract

Abstract:In this study we describe a beads-based assay for rapid, sensitive and specific isolation and detection of influenza vaccine hemagglutinin (HA). Amplification of the hemagglutinin signal resulted from binding of an electrochemical label as quantum dots (QDs). For detection of the metal and protein part of the resulting HA-CdTe complex, two differential pulse voltammetric methods were used. The procedure includes automated robotic isolation and electrochemical analysis of the isolated product. The isolation procedure was based on the binding of paramagnetic particles (MPs) with glycan (Gly), where glycan was used as the specific receptor for linkage of the QD-labeled hemagglutinin.

Complexes of Metal-Based Nanoparticles with Chitosan Suppressing the Risk of Staphylococcus aureus and Escherichia coli Infections

“…The most frequent types of QDs synthesized using microwave irradiation are CdTe, CdSe, Zn 12x Cd x Se, and ZnSe. These QDs are mostly covered with thiol ligands such as mercaptopropionic acid, mercaptosuccinic acid, or glutathion (Qian et al, 2006;Ryvolova et al, 2012;Krejcova et al, 2013).…”

Nanomaterial-based biosensors for detection of pathogenic virus

“…Krejcova and coworkers [29] proposed an influenza virus detection sensor as a pandemic threat by using a 3D microfluidic chip, applied for influenza hemagglutinin. This method was based on two different steps, (i) specific isolation, after labeling HA with CdS-QDs, and conjugate them on a surface of glycan-modified MPs and (ii) voltammetry based detection step.…”

“…One of the unique properties of GO is the ability of being host of stranded DNA to make aptasensors. A research conducted by Bao et al [40] about immobilizing DNA strand on GO provided the way to make a label free aptasensor for detection of viruses This novel procedure for the designing of sensors had some significant points; (i) There was no need to decoration of GO towards immobilization, (ii) Comparing with other apatasensors, it was unnecessary to label [29] DNA. (iii) The device was also used for the biosensing of nanostructures.…”

Nanoscale virus biosensors: state of the art

“…19,32 There are two main biorecognition strategies: detection of viral nucleic acid (NA) sequence [32][33][34] and detection of specific viral biomolecules such as surface proteins/antigens. [35][36][37] Nanotechnology-based biosensors show high specificity and sensitivity after labeling with NA probe, antibody, or other specific molecule with affinity to the target structure. 38 …”

“…A wide range of electrochemical methods can be used for detection include differential pulse voltammetry (DPV), 35,80,82 square wave voltammetry (SWV), 83,84 cyclic voltammetry (CV), [85][86][87] and conductometry. 88,89 Also, impedance is widely used as a detection method in biosensors.…”