Beam induced deposition of 3D electrodes to improve coupling to cells

Martiradonna, Luigi; Quarta, Luca; Sileo, Leonardo; Schertel, Andreas; Maccione, Alessandro; Simi, Alessandro; Dante, Silvia; Scarpellini, Alice; Berdondini, Luca; Vittorio, Massimo De

doi:10.1016/j.mee.2012.03.027

Cited by 9 publications

(4 citation statements)

References 11 publications

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“…resist and is a result of an antecedent lithography process (Ozel et al 2017). Alternatively, assisted deposition can be used to construct the vertical feature from the bottom-up (Martiradonna et al 2012; C. Xie et al 2012).…”

Section: Fabricationmentioning

confidence: 99%

“…Furthermore, in the case of solid inflexible vertical structures coupled to cells with long protrusions, i.e. neuronal cells, a clear directionality effect has been shown as these cells grow on large pillar arrays made of gold (Panaitov et al 2011; Santoro, Panaitov, and Offenhäusser 2014), platinum (Martiradonna et al 2012; Sileo et al 2013), and indium phosphide (Gautam et al 2017). In particular, it has been shown that neuronal cells can grow on vertical structures and form tight junctions at the nanopillar surface.…”

Section: Resolution Of the Cell-vertical Structure Interfacementioning

confidence: 99%

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Interfacing Cells with Vertical Nanoscale Devices: Applications and Characterization

McGuire

Santoro

Cui

2018

Annual Rev. Anal. Chem.

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Measurements of the intracellular state of mammalian cells often require probes or molecules to breach the tightly regulated cell membrane. Mammalian cells have been shown to grow well on vertical nanoscale structures in vitro, going out of their way to reach and tightly wrap the structures. A great deal of research has taken advantage of this interaction to bring probes close to the interface or deliver molecules with increased efficiency or ease. In turn, techniques have been developed to characterize this interface. Here, we endeavor to survey this research with an emphasis on the interface as driven by cellular mechanisms.

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Section: Fabricationmentioning

confidence: 99%

Section: Resolution Of the Cell-vertical Structure Interfacementioning

confidence: 99%

Interfacing Cells with Vertical Nanoscale Devices: Applications and Characterization

McGuire

Santoro

Cui

2018

Annual Rev. Anal. Chem.

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“…Although no studies have attempted to optimize IN-CELL recordings by increasing the size of gMμEs, several laboratories have explored the potential use of nanometric size mushroom shaped electrodes 32 34 36 . These studies are based on the reasoning that nanometric sized mushroom-shaped microelectrodes may be more suitable for interfacing with small (10–20 μm diameter) mammalian neurons than the very large Aplysia neurons (50–80 μm diameter) used in our proof-of-concept studies.…”

mentioning

confidence: 99%

A feasibility study of multi-site,intracellular recordings from mammalian neurons by extracellular gold mushroom-shaped microelectrodes

Ojovan

Rabieh

Shmoel

et al. 2015

Sci Rep

103

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The development of multi-electrode array platforms for large scale recording of neurons is at the forefront of neuro-engineering research efforts. Recently we demonstrated, at the proof-of-concept level, a breakthrough neuron-microelectrode interface in which cultured Aplysia neurons tightly engulf gold mushroom-shaped microelectrodes (gMμEs). While maintaining their extracellular position, the gMμEs record synaptic- and action-potentials with characteristic features of intracellular recordings. Here we examined the feasibility of using gMμEs for intracellular recordings from mammalian neurons. To that end we experimentally examined the innate size limits of cultured rat hippocampal neurons to engulf gMμEs and measured the width of the “extracellular” cleft formed between the neurons and the gold surface. Using the experimental results we next analyzed the expected range of gMμEs-neuron electrical coupling coefficients. We estimated that sufficient electrical coupling levels to record attenuated synaptic- and action-potentials can be reached using the gMμE-neuron configuration. The definition of the engulfment limits of the gMμEs caps diameter at ≤2–2.5 μm and the estimated electrical coupling coefficients from the simulations pave the way for rational development and application of the gMμE based concept for in-cell recordings from mammalian neurons.

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“…The success of the numerous extracellular interface concepts proposed in basic science is limited by problems ranging from improper cell adhesion to inadequate device stability . To overcome such limitations, the design of engineered interfaces , is mainly based on successful application of emerging technologies, such as nanotechnology. − The interface between cells and nanostructures has been studied extensively. − In particular, the morphology of cells interfacing 3D nanoelectrodes has been investigated in detail, − as these are prominent candidates to solve the aforementioned problems. Potential mechanisms for coupling between electrogenic cells and multiple 3D nano- and microstructures are proposed and used for extracellular applications. ,,,,− Most of these 3D nano- and microstructures can be classified under two main types: cylindrical pillars with and without a cap.…”

mentioning

confidence: 99%

Interfacing Electrogenic Cells with 3D Nanoelectrodes: Position, Shape, and Size Matter

et al. 2014

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An in-depth understanding of the interface between cells and nanostructures is one of the key challenges for coupling electrically excitable cells and electronic devices. Recently, various 3D nanostructures have been introduced to stimulate and record electrical signals emanating from inside of the cell. Even though such approaches are highly sensitive and scalable, it remains an open question how cells couple to 3D structures, in particular how the engulfment-like processes of nanostructures work. Here, we present a profound study of the cell interface with two widely used nanostructure types, cylindrical pillars with and without a cap. While basic functionality was shown for these approaches before, a systematic investigation linking experimental data with membrane properties was not presented so far. The combination of electron microscopy investigations with a theoretical membrane deformation model allows us to predict the optimal shape and dimensions of 3D nanostructures for cell-chip coupling.

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Beam induced deposition of 3D electrodes to improve coupling to cells

Cited by 9 publications

References 11 publications

Interfacing Cells with Vertical Nanoscale Devices: Applications and Characterization

Interfacing Cells with Vertical Nanoscale Devices: Applications and Characterization

A feasibility study of multi-site,intracellular recordings from mammalian neurons by extracellular gold mushroom-shaped microelectrodes

Interfacing Electrogenic Cells with 3D Nanoelectrodes: Position, Shape, and Size Matter

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