Beauveria bassiana: Endophytic colonization and plant disease control

Ownley, Bonnie H.; Griffin, Mary Ruth; Klingeman, William E.; Gwinn, Kimberly D.; Moulton, John K.; Pereira, Roberto M.

doi:10.1016/j.jip.2008.01.010

Cited by 347 publications

(270 citation statements)

References 22 publications

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“…Only recently, however, have fungal entomopathogens been shown to occur as endophytes, both naturally and in response to various inoculation methods 2 . The ecological function of endophytic fungal entomopathogens remains largely unknown, but some studies have implicated them in plant growth 3,4 , herbivore resistance [5][6][7][8] , and disease resistance 9,10 . The overall objective of the methods presented here is to introduce a fungal entomopathogen as an endophyte, in preparation for subsequent evaluations of endophytic biological control.…”

Section: Introductionmentioning

confidence: 99%

“…Beauveria bassiana (Balsamo) Vullemin (Ascomycota: Hypocreales) is the best-studied endophytic fungal entomopathogen [5][6][7][8][9][11][12][13][14][15][16][17][18][19] , and it is available as a commercial mycopesticide. Inoculation methods tested to establish B. bassiana as an endophyte include soil drenches 14,17 , seed coatings 18 and immersions 14 , radicle dressings 13,15 , root and rhizome immersions 11,16,18 , .…”

Section: Introductionmentioning

confidence: 99%

“…Inoculation methods tested to establish B. bassiana as an endophyte include soil drenches 14,17 , seed coatings 18 and immersions 14 , radicle dressings 13,15 , root and rhizome immersions 11,16,18 , . Recent evidence suggests that endophytic B. bassiana has the potential to protect plants not only from arthropod pests [5][6][7][22][23][24][25][26][27] , but also from some plant pathogens 9 .…”

Section: Introductionmentioning

confidence: 99%

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Establishing Fungal Entomopathogens as Endophytes: Towards Endophytic Biological Control

Parsa

Ortiz

Vega

2013

JoVE

124

123

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Beauveria bassiana is a fungal entomopathogen with the ability to colonize plants endophytically. As an endophyte, B. bassiana may play a role in protecting plants from herbivory and disease. This protocol demonstrates two inoculation methods to establish B. bassiana endophytically in the common bean (Phaseolus vulgaris), in preparation for subsequent evaluations of endophytic biological control. Plants are grown from surface-sterilized seeds for two weeks before receiving a B. bassiana treatment of 10 8 conidia/ml (or water) applied either as a foliar spray or a soil drench. Two weeks later, the plants are harvested and their leaves, stems and roots are sampled to evaluate endophytic fungal colonization. For this, samples are individually surface sterilized, cut into multiple sections, and incubated in potato dextrose agar media for 20 days. The media is inspected every 2-3 days to observe fungal growth associated with plant sections and record the occurrence of B. bassiana to estimate the extent of its endophytic colonization. Analyses of inoculation success compare the occurrence of B. bassiana within a given plant part (i.e. leaves, stems or roots) across treatments and controls. In addition to the inoculation method, the specific outcome of the experiment may depend on the target crop species or variety, the fungal entomopathogen species strain or isolate used, and the plant's growing conditions.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Establishing Fungal Entomopathogens as Endophytes: Towards Endophytic Biological Control

Parsa

Ortiz

Vega

2013

JoVE

124

123

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“…Fungal endophytes play an important role in protecting plants against herbivorous insects (Jallow et al 2004(Jallow et al , 2008 and plant pathogens (Ownley et al 2008). Beauveria bassiana has been reported as an endophyte in a variety of plants, including maize Lewis 1991, 1992a, b;Wagner and Lewis 2000), Theobroma gileri (Evans et al 2003), Carpinus caroliniana (Bills and Polishook 1991), in seeds and needles of Pinus monticola (Ganley and Newcombe 2005) in opium poppies (Quesada-Moraga et al 2006), in banana tissue cultures (Akello et al 2007), and in coffee (Posada et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Effect of inoculation method and plant growth medium on endophytic colonization of sorghum by the entomopathogenic fungus Beauveria bassiana

2009

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A study was conducted to determine the effect of inoculation method and plant growth medium on colonization of sorghum by an endophytic Beauveria bassiana. Colonization of leaves, stems, and roots by B. bassiana was assessed 20-days after application of the fungus. Although B. bassiana established as an endophyte in sorghum leaves, stems, and roots regardless of inoculation method (leaf, seed, or soil inoculation), plant growth medium (sterile soil, non-sterile soil, or vermiculite) apparently influenced colonization rates. Seed inoculation with conidia caused no stem or leaf colonization by the fungus in non-sterile soil but did result in substantial endophytic colonization in vermiculite and sterile soil. Leaf inoculation did not result in root colonization, regardless of plant growth medium. Endophytic colonization was greater in leaves and stems than roots. Endophytic colonization by B. bassiana had no adverse effects on the growth of sorghum plants. Leaf inoculation with a conidial suspension proved to be the best method to introduce B. bassiana into sorghum leaves for plants growing in either sterile or nonsterile soil. Further research should focus on the virulence of endophytic B. bassiana against sorghum stem borers.

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“…In recent years, B. bassiana considered as a dual purpose bioagent against both insect pests and plant pathogens. Isolates of B. bassiana inhibited mycelial growth of an array of soilborne and foliar plant pathogens in vitro (Ownley et al, 2008). Hence, it is essential to assess genetic variation among B. bassiana isolates and to determine their distribution, abundance and potential for genetic exchange between and within populations.…”

Section: Introductionmentioning

confidence: 99%

Analysis of genetic diversity among different isolates of Beauveria bassiana by RAPD-PCR

Prabhukarthikeyan

Keerthana

Archana

et al. 2017

Journal of Biological Control

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ABSTRACT:The genetic diversity of Beauveria bassiana isolated from the different ecosystem of Tamil Nadu was analyzed. Totally fifteen isolates were used in this study. The genetic variability and relationship among 15 isolates were analyzed using 15 Random Amplified Polymorphic DNA (RAPD) markers. Among 15 primers used, six primers viz., OPA02, OPF01, OPX07, OPA03, OPZ19 and OPG19 showed 100 percent polymorphism. The relationships among the isolates were examined and represented as dendrogram by using UP-GMA clusters. Results revealed that similarity coefficients of B. bassiana isolates based on RAPD markers ranged from 42.00 to 86.00 percent. The maximum of 85 percent similarity was observed between the isolates B23 and B24. All isolates have significantly different from one another. Overall we concluded that RAPD was found to be better in assessing genetic diversity among B. bassiana isolates.

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Beauveria bassiana: Endophytic colonization and plant disease control

Cited by 347 publications

References 22 publications

Establishing Fungal Entomopathogens as Endophytes: Towards Endophytic Biological Control

Establishing Fungal Entomopathogens as Endophytes: Towards Endophytic Biological Control

Effect of inoculation method and plant growth medium on endophytic colonization of sorghum by the entomopathogenic fungus Beauveria bassiana

Analysis of genetic diversity among different isolates of Beauveria bassiana by RAPD-PCR

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