Beef carcass microbiota after slaughtering and primary cooling: A metataxonomic assessment to infer contamination drivers

Botta, C.; Franciosa, I.; Coisson, J.D.; Ferrocino, I.; Colasanto, A.; Arlorio, M.; Cocolin, L.; Rantsiou, K.

doi:10.1016/j.foodres.2023.113466

Cited by 4 publications

(1 citation statement)

References 50 publications

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“…Studies on the co-existence network among meat plant microbiota are very scarce. Botta et al recently explored the co-occurrence network for bacteria both on beef carcass and in slaughterhouse environments ( Botta et al, 2022 , 2023 ). The mechanisms mediating the pairwise co-existence/occurrence in both studies and those in our analysis are not clear.…”

Section: A Meta-analysis Of Persistent Bacteria In Meat Processing En...mentioning

confidence: 99%

Driving forces shaping the microbial ecology in meat packing plants

Yang,

Narvaez-Bravo,

Zhang

2024

Front. Microbiol.

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Meat production is a complex system, continually receiving animals, water, air, and workers, all of which serve as carriers of bacteria. Selective pressures involved in different meat processing stages such as antimicrobial interventions and low temperatures, may promote the accumulation of certain residential microbiota in meat cutting facilities. Bacteria including human pathogens from all these sources can contaminate meat surfaces. While significant advancements have been made in enhancing hygienic standards and pathogen control measures in meat plants, resulting in a notable reduction in STEC recalls and clinical cases, STEC still stands as a predominant contributor to foodborne illnesses associated with beef and occasionally with pork. The second-and third-generation sequencing technology has become popular in microbiota related studies and provided a better image of the microbial community in the meat processing environments. In this article, we reviewed the potential factors influencing the microbial ecology in commercial meat processing facilities and conducted a meta-analysis on the microbiota data published in the last 10 years. In addition, the mechanisms by which bacteria persist in meat production environments have been discussed with a focus on the significant human pathogen E. coli O157:H7 and generic E. coli, an indicator often used for the hygienic condition in food production.

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Section: A Meta-analysis Of Persistent Bacteria In Meat Processing En...mentioning

confidence: 99%

Driving forces shaping the microbial ecology in meat packing plants

Yang,

Narvaez-Bravo,

Zhang

2024

Front. Microbiol.

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Impact of dry aging on quality parameters and microbiological safety of beef

Savini,

Indio,

Panseri

et al. 2024

LWT

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Microbial contamination pathways in a poultry abattoir provided clues on the distribution and persistence of Arcobacter spp.

Botta,

Buzzanca,

Chiarini

et al. 2024

Appl Environ Microbiol

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The consumption of contaminated poultry meat is a significant threat for public health, as it implicates in foodborne pathogen infections, such as those caused by Arcobacter . The mitigation of clinical cases requires the understanding of contamination pathways in each food process and the characterization of resident microbiota in the productive environments, so that targeted sanitizing procedures can be effectively implemented. Nowadays these investigations can benefit from the complementary and thoughtful use of culture- and omics-based analyses, although their application in situ is still limited. Therefore, the 16S-rRNA gene-based sequencing of total DNA and the targeted isolation of Arcobacter spp. through enrichment were performed to reconstruct the environmental contamination pathways within a poultry abattoir, as well as the dynamics and distribution of this emerging pathogen. To that scope, broiler’s neck skin and caeca have been sampled during processing, while environmental swabs were collected from surfaces after cleaning and sanitizing. Metataxonomic survey highlighted a negligible impact of fecal contamination and a major role of broiler’s skin in determining the composition of the resident abattoir microbiota. The introduction of Arcobacter spp. in the environment was mainly conveyed by this source rather than the intestinal content. Arcobacter butzleri represented one of the most abundant species and was extensively detected in the abattoir by both metataxonomic and enrichment methods, showing higher prevalence than other more thermophilic Campylobacterota. In particular, Arcobacter spp. was recovered viable in the plucking sector with high frequency, despite the adequacy of the sanitizing procedure. IMPORTANCE Our findings have emphasized the persistence of Arcobacter spp. in a modern poultry abattoir and its establishment as part of the resident microbiota in specific environmental niches. Although the responses provided here are not conclusive for the identification of the primary source of contamination, this biogeographic assessment underscores the importance of monitoring Arcobacter spp. from the early stages of the production chain with the integrative support of metataxonomic analysis. Through such combined detection approaches, the presence of this pathogen could be soon regarded as hallmark indicator of food safety and quality in poultry slaughtering.

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Beef carcass microbiota after slaughtering and primary cooling: A metataxonomic assessment to infer contamination drivers

Cited by 4 publications

References 50 publications

Driving forces shaping the microbial ecology in meat packing plants

Driving forces shaping the microbial ecology in meat packing plants

Impact of dry aging on quality parameters and microbiological safety of beef

Microbial contamination pathways in a poultry abattoir provided clues on the distribution and persistence of Arcobacter spp.

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