Behavior and the cholinergic parameters in olfactory bulbectomized female rodents: Difference between rats and mice

Stepanichev, M. Yu.; Markov, Daniil A.; Pasikova, N. V.; Gulyaeva, N. V.

doi:10.1016/j.bbr.2015.09.033

Cited by 11 publications

(6 citation statements)

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“…OBX model is associated with increment of immobility time in the forced swimming test and anhedonic-like response in sucrose preference ( Rinwa and Kumar, 2014 ). Olfactory bulbectomized rats demonstrated depressive-like behavioral marks, such as the decreased sucrose consumption, hyperactivity, impaired short-term memory, and anxiety-like behavioral features ( Stepanichev et al, 2016 ). Treadmill exercise restored sucrose consumption with increased cell proliferation and decreased apoptotic cell death in the depressive rats ( Lee et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Treadmill exercise improves depression-like symptoms by enhancing serotonergic function through upregulation of 5-HT1A expression in the olfactory bulbectomized rats

et al. 2017

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The olfactory bulbectomy (OBX) is a well-known method inducing animal model of depression. Depression is associated with dysfunction of serotonin (5-hydroxytryptamine, 5-HT) system. In the present study, antidepressive effect of treadmill exercise was investigated using olfactory bulbectomized rats. After bilateral bulbectomy, the rats in the treadmill exercise groups were subjected to run on a treadmill for 30 min once a day during 28 days. Increased immobility time and decreased fast time in the forced swim test were observed in the olfactory bulbectomized rats. Sucrose preference in the sucrose preference test was decreased and activity in the open field test was also increased in the olfactory bulbectomized rats. Treadmill exercise decreased immobility time and activity and increased fast time and sucrose preference in the olfactory bulbectomized rats. Expressions of 5-HT and tryptophan hydroxylase (TPH) in the dorsal raphe of rats were suppressed by OBX and treadmill exercise increased the expressions of 5-HT and TPH in the olfactory bulbectomized rats. Serotonin receptor type 1A (5-HT1A) expression in the dorsal raphe was reduced by OBX and treadmill exercise increased 5-HT1A expression in the olfactory bulbectomized rats. In the present study, treadmill exercise ameliorated OBX-induced depressive symptoms. The antidepressive effect of treadmill exercise might be ascribed to the enhancement of serotonergic function through upregulation of 5-HT1A expression in the dorsal raphe.

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Section: Discussionmentioning

confidence: 99%

Treadmill exercise improves depression-like symptoms by enhancing serotonergic function through upregulation of 5-HT1A expression in the olfactory bulbectomized rats

et al. 2017

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“…Bilateral olfactory bulbectomy (OB), a well-established animal model of depression (Redmond et al, 1997; Song and Leonard, 2005), results in a series of behavioral and neurochemical alterations comparable with those observed in depression patients, such as enhanced locomotor response to stress (e.g., enhanced locomotor and rearing behaviors in open field test; Redmond et al, 1997; Dandekar et al, 2009; Morales-Medina et al, 2012), decreased sucrose preference (Romeas et al, 2009; Stepanichev et al, 2016), extended floating time in the forced swim test (Morales-Medina et al, 2012; Zhang et al, 2016) and abnormal changes of brain serotonergic, noradrenergic, glutamatergic, dopaminergic and GABAergic systems (Song and Leonard, 2005). Although the mechanism of OB-induced depressive-like behavior is complex, most of the above changes can be reversed by chronic antidepressants treatment (Cryan and Mombereau, 2004; Song and Leonard, 2005), and it is one of the most reliable depression animal models, at least in rats (Willner and Mitchell, 2002).…”

Section: Introductionmentioning

confidence: 94%

Impaired Spinal Glucocorticoid Receptor Signaling Contributes to the Attenuating Effect of Depression on Mechanical Allodynia and Thermal Hyperalgesia in Rats with Neuropathic Pain

Xiao

Sun

Luo

2017

Front. Cell. Neurosci.

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Although depression-induced altered pain perception has been described in several laboratory and clinical studies, its neurobiological mechanism in the central nervous system (CNS), particularly in the spinal dorsal horn, remains unclear. Therefore, in this study, we aimed to clarify whether nociceptive sensitivity of neuropathic pain is altered in the olfactory bulbectomy (OB) model of depression and whether glucocorticoid receptor (GR), which is involved in the etio-pathologic mechanisms of both major depression and neuropathic pain, contributes to these processes in the spinal dorsal horn of male Sprague-Dawley rats. The results showed that mechanical allodynia and thermal hyperalgesia induced by spinal nerve ligation (SNL) were attenuated in OB-SNL rats with decreased spinal GR expression and nuclear translocation, whereas non-olfactory bulbectomy (NOB)-SNL rats showed increased spinal GR nuclear translocation. In addition, decreased GR nuclear translocation with normal mechanical nociception and hypoalgesia of thermal nociception were observed in OB-Sham rats. Intrathecal injection (i.t.) of GR agonist dexamethasone (Dex; 4 μg/rat/day for 1 week) eliminated the attenuating effect of depression on nociceptive hypersensitivity in OB-SNL rats and aggravated neuropathic pain in NOB-SNL rats, which was associated with the up-regulation of brain-derived neurotrophic factor (BDNF), TrkB and NR2B expression in the spinal dorsal horn. The present study shows that depression attenuates the mechanical allodynia and thermal hyperalgesia of neuropathic pain and suggests that altered spinal GR-BDNF-TrkB signaling may be one of the reasons for depression-induced hypoalgesia.

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“…The remaining mice were kept to investigate the survival time over a 100-day period. Tumor growth, animal health and behavior including self-centered behavior, motivation, anhedonia, anxiety and despair behavior were monitored every five days as described previously (22). Mice were sacrificed by cervical dislocation when the tumor diameter reached 15 mm.…”

Section: Reverse-transcription Quantitative Pcr (Rt-qpcr)mentioning

confidence: 99%

Total flavonoids suppress lung cancer growth via the COX‑2‑mediated Wnt/β‑catenin signaling pathway

Han

Fang

et al. 2020

Oncol Lett

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The aim of the present study was to explore the anti-cancer effects of total flavonoids (TF) on lung cancer and to investigate the underlying mechanism. The inhibitory effect of TF on the proliferation of A549 cells in vitro was measured using an MTT assay. The apoptotic rate of TF-treated A549 cells was analyzed using flow cytometry and terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling. Migration and invasion assays were performed to investigate the anti-migration effect of TF on A549 cells. Reverse-transcription quantitative PCR was used to analyze BCL2-like 2, BCL2, Bax, Bad, cyclooxygenase 2 (COX-2), Wnt and β-catenin mRNA expression levels in A549 cells. The in vivo anti-cancer effect of TF was investigated in a subcutaneous xenograft model of lung cancer in BALB/c nude mice. The results obtained in the present study revealed that TF exerted a significant inhibitory effect on the proliferation of A549 cells in a dose-dependent manner (P<0.01). TF induced apoptosis of A549 cells, which exhibited increased and decreased expression of pro-and anti-apoptotic genes, respectively. Furthermore, TF had a significant inhibitory effect on the migration and invasion of A549 cells (P<0.01). The mRNA expression levels of COX-2, Wnt and β-catenin were significantly downregulated in TF-treated A549 cells compared with controls. Additionally, treatment with TF inhibited tumor growth in mice, with a tumor inhibition rate of 64.07% compared with the controls. TF exhibited significant tumor inhibitory effects in vivo by promoting the apoptosis of tumor cells. In conclusion, the results suggested that TF may regulate lung cancer growth via the COX-2-Wnt/β-catenin signaling pathway. TF may serve as a novel anti-cancer agent for the treatment of lung cancer.

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Behavior and the cholinergic parameters in olfactory bulbectomized female rodents: Difference between rats and mice

Cited by 11 publications

References 68 publications

Treadmill exercise improves depression-like symptoms by enhancing serotonergic function through upregulation of 5-HT1A expression in the olfactory bulbectomized rats

Treadmill exercise improves depression-like symptoms by enhancing serotonergic function through upregulation of 5-HT1A expression in the olfactory bulbectomized rats

Impaired Spinal Glucocorticoid Receptor Signaling Contributes to the Attenuating Effect of Depression on Mechanical Allodynia and Thermal Hyperalgesia in Rats with Neuropathic Pain

Total flavonoids suppress lung cancer growth via the COX‑2‑mediated Wnt/β‑catenin signaling pathway

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