Fatigue cracking by loading is one of the main mechanisms of damage to asphalt mixtures in service. Several studies worldwide have been conducted to try to understand the response that hot-mix asphalt undergo under this mechanism of damage. Despite the above, the fatigue phenomenon in asphalt mixtures is still not fully understood. The current research hypothesizes that the response under repeated loading of asphalt mixtures in fatigue tests can be more clearly understood through the one obtained under monotonic loading. For this reason, this study presents the results of the first phase of the research in which beams of asphalt mixtures were subjected to flexion using monotonic loads. The above, to correlate the evaluated properties with those obtained in a second phase where the response of the beams under repeated load (fatigue) will be measured. Beams made of two hot-mix asphalt mixes, two asphalt contents, and two different thicknesses were subjected to flexural strength tests. From the tests, the modulus of rupture, the maximum monotonic load that supports the beams in the failure state, the displacement in the failure state, and the relation between load and displacement were obtained. As a general conclusion of the study, it was obtained that the response experienced by the beams subjected to monotonic load has a broad correlation with the reported in the reference literature.