Behavior of N-acylated daunorubicins in MDR1 gene transfected and parental cells

Aszalós, Adorján; Pine, P. Scott; Pandey, Ramesh C.; Gottesman, Michael M.

doi:10.1016/0006-2952(95)00209-i

Cited by 6 publications

(1 citation statement)

References 11 publications

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“…The L5178 mouse T cell lymphoma cell line was derived from the parental L5178Y mouse T cell lymphoma cell line after the latter was infected with the plasmid pHa that contained the gene MDR1/A thereby rendering the progeny resistant to multiple anticancer drugs as previously described (Aszalos et al ., 1995). MDR1 expressing cells were selected with the use of 60 µ g/L colchicine (Sigma Aldrich Química SA, Madrid, Spain) that maintains the expression of the MDR phenotype as evident from the ability of the cells to grow in the presence of cancer chemotherapeutics such as epirubicin to which the parental L5178Y cells were susceptible.…”

Section: Culture Of L5178 Mdr Mouse T Cell Lymphoma Cell Linementioning

confidence: 99%

Carpobrotus edulis methanol extract inhibits the MDR efﬂux pumps, enhances killing of phagocytosed S. aureus and promotes immune modulation

Ordway

Hohmann

Viveiros

et al. 2003

Phytotherapy Research

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Although alkaloids from the family Aizoaceae have anticancer activity, species of this family have received little attention. Because these alkaloids also exhibit properties normally associated with compounds that have activity at the level of the plasma membrane, a methanol extract of Carpobrotus edulis, a common plant found along the Portuguese coast, was studied for properties normally associated with plasma membrane active compounds. The results of this study show that the extract is non-toxic at concentrations that inhibit a verapamil sensitive efflux pump of L5178 mouse T cell lymphoma cell line thereby rendering these multi-drug resistant cells susceptible to anticancer drugs. These non-toxic concentrations also prime THP-1 human monocyte-derived macrophages to kill ingested Staphylococcus aureus and to promote the release of lymphokines associated with cellular immune functions. The extract also induces the proliferation of THP-1 cells within 1 day of exposure to quantities normally associated with phytohaemagglutinin. The potential role of the compound(s) isolated from this plant in cancer biology is intriguing and is currently under investigation. It is supposed that the resistance modifier and immunomodulatory effect of this plant extract can be exploited in the experimental chemotherapy of cancer and bacterial or viral infections.

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Section: Culture Of L5178 Mdr Mouse T Cell Lymphoma Cell Linementioning

confidence: 99%

Carpobrotus edulis methanol extract inhibits the MDR efﬂux pumps, enhances killing of phagocytosed S. aureus and promotes immune modulation

Ordway

Hohmann

Viveiros

et al. 2003

Phytotherapy Research

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show abstract

Effect of combination of suboptimal concentrations of P-glycoprotein blockers on the proliferation ofMDR1 gene expressing cells

et al. 1996

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Pharmacologically active in vivo doses of P-glycoprotein (Pgp) blockers, specifically verapamil, Cremophor EL and PSC833 cause toxicity in addition to that from the concomitantly used cancer chemotherapeutic drugs. It was shown before that these blockers cause different types of toxicities in vivo. We found that these 3 chemically distinct Pgp blockers exert different biophysical effects on the membranes of LIZ10 MDR cells. They also affect the general metabolism of these cells differently, but all block affinity labeling of Pgp. We could also show that the combination of suboptimal doses of these blockers can restore the uptake of the Pgp substrate rhodamine I23 into L I 2 I OMDR, 3T3MDR and KB-VI cells and can reduce the survival rate of these cells when treated in combination with daunorubicin. Our results suggest that the combination of suboptimal doses of these Pgp blockers may be advantageous in clinical practice.

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Anti-psychotic drugs reverse multidrug resistance of tumor cell lines and human AML cells ex-vivo

Szabó¹,

Szabó²,

Ocsovszki³

et al. 1999

Cancer Letters

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Behavior of N-acylated daunorubicins in MDR1 gene transfected and parental cells

Cited by 6 publications

References 11 publications

Carpobrotus edulis methanol extract inhibits the MDR efﬂux pumps, enhances killing of phagocytosed S. aureus and promotes immune modulation

Carpobrotus edulis methanol extract inhibits the MDR efﬂux pumps, enhances killing of phagocytosed S. aureus and promotes immune modulation

Effect of combination of suboptimal concentrations of P-glycoprotein blockers on the proliferation ofMDR1 gene expressing cells

Anti-psychotic drugs reverse multidrug resistance of tumor cell lines and human AML cells ex-vivo

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