Behavior of neurons in the abducens nucleus of the alert cat—III. Axotomized motoneurons

Delgado‐García, José M.; Pozo, Francisco del; Spencer, Robert F.; Baker, R.

doi:10.1016/0306-4522(88)90319-3

Cited by 90 publications

(84 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It can be stressed the idea that exogenous trophic support switches the cellular program that maintains the axotomized cell synaptically disconnected from the afferent circuit to a cellular program which implies the reinnervation by afferents. Previous findings in the axotomized abducens motoneurons and internuclear neurons, also in the cat, support the idea of a strong dependence of these neurons on their target as demonstrated by the recovery of firing properties in coincidence with target reinnervation (Baker et al, 1981;Delgado-García, 1988;Benítez-Temiño et al, 2005).…”

Section: Alterations Of Target-derived Trophic Support Determine the supporting

confidence: 67%

“…For the delivery of neurotrophins, we implanted the stump of the abducens nerve in a custom-made chamber to control the milieu that bathed it and to prevent axonal reinnervation of any orbital structure or other extraocular muscles (Delgado-García et al, 1988). The intraorbitary device consisted of a chamber that had two pieces of tubing inserted for fluid replacement and two electrodes as we recently described (Davis-Ló pez de Carrizosa et al, 2008).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Complementary Actions of BDNF and Neurotrophin-3 on the Firing Patterns and Synaptic Composition of Motoneurons

Carrizosa¹,

Morado-Díaz²,

Tena³

et al. 2009

J. Neurosci.

View full text Add to dashboard Cite

Neurotrophins, as target-derived factors, are essential for neuronal survival during development, but during adulthood, their scope of actions widens to become also mediators of synaptic and morphological plasticity. Target disconnection by axotomy produces an initial synaptic stripping ensued by synaptic rearrangement upon target reinnervation. Using abducens motoneurons of the oculomotor system as a model for axotomy, we report that trophic support by brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) or a mixture of both, delivered to the stump of severed axons, results in either the prevention of synaptic stripping when administered immediately after lesion or in a promotion of reinnervation of afferents to abducens motoneurons once synaptic stripping had occurred, in concert with the recovery of synaptic potentials evoked from the vestibular nerve. Synaptotrophic effects, however, were larger when both neurotrophins were applied together. The axotomy-induced reduction in firing sensitivities related to eye movements were also restored to normal values when BDNF and NT-3 were administered, but discharge characteristics recovered in a complementary manner when only one neurotrophin was used. This is the first report to show selective retrograde trophic dependence of circuit-driven firing properties in vivo indicating that NT-3 restored the phasic firing, whereas BDNF supported the tonic firing of motoneurons during eye movement performance. Therefore, our data report a link between the synaptotrophic actions of neurotrophins, retrogradely delivered, and the alterations of neuronal firing patterns during motor behaviors. These trophic actions could be responsible, in part, for synaptic rearrangements that alter circuit stability and synaptic balance during plastic events of the brain.

show abstract

Section: Alterations Of Target-derived Trophic Support Determine the supporting

confidence: 67%

Section: Methodsmentioning

confidence: 99%

Complementary Actions of BDNF and Neurotrophin-3 on the Firing Patterns and Synaptic Composition of Motoneurons

Carrizosa¹,

Morado-Díaz²,

Tena³

et al. 2009

J. Neurosci.

View full text Add to dashboard Cite

show abstract

“…3B), but not that of internuclear neurons (data not shown), was perturbed after VIth nerve axotomy (Delgado-García et al, 1988; Davis-Ló pez de Carrizosa et at., 2009). Changes took place over the first week and did not recover during the entire survival time (Fig.…”

Section: Ngf Reinstates the Firing Pattern Of Axotomized Abducens Motmentioning

confidence: 99%

“…These alterations revert to normal following reinnervation of natural or exogenous targets (Delgado-García et al, 1988;Benítez-Temiño et al, 2005) or when BDNF and NT-3 are exogenously applied (Davis-Ló pez de Carrizosa et al, 2009). A possible role for NGF in regulating the morphophysiological characteristics of extraocular motoneurons and its afferent inputs has not yet been studied.…”

Section: Introductionmentioning

confidence: 99%

Nerve Growth Factor Regulates the Firing Patterns and Synaptic Composition of Motoneurons

Carrizosa¹,

Morado-Díaz²,

Morcuende³

et al. 2010

Journal of Neuroscience

View full text Add to dashboard Cite

Target-derived neurotrophins exert powerful synaptotrophic actions in the adult brain and are involved in the regulation of different forms of synaptic plasticity. Target disconnection produces a profound synaptic stripping due to the lack of trophic support. Consequently, target reinnervation leads to synaptic remodeling and restoration of cellular functions. Extraocular motoneurons are unique in that they normally express the TrkA neurotrophin receptor in the adult, a feature not seen in other cranial or spinal motoneurons, except after lesions such as axotomy or in neurodegenerative diseases like amyotrophic lateral sclerosis. We investigated the effects of nerve growth factor (NGF) by retrogradely delivering this neurotrophin to abducens motoneurons of adult cats. Axotomy reduced the density of somatic boutons and the overall tonic and phasic firing modulation. Treatment with NGF restored synaptic inputs and firing modulation in axotomized motoneurons. When K252a, a selective inhibitor of tyrosine kinase activity, was applied to specifically test TrkA effects, the NGF-mediated restoration of synapses and firing-related parameters was abolished. Discharge variability and recruitment threshold were, however, increased by NGF compared with control or axotomized motoneurons. Interestingly, these parameters returned to normal following application of REX, an antibody raised against neurotrophin receptor p75 (p75 NTR ). In conclusion, NGF, acting retrogradely through TrkA receptors, supports afferent boutons and regulates the burst and tonic signals correlated with eye movements. On the other hand, p75 NTR activation regulates recruitment threshold, which impacts on firing regularity. To our knowledge, this is the first report showing powerful synaptotrophic effects of NGF on motoneurons in vivo.

show abstract

“…Such an elimination can be induced by axon transection, which is followed by an extensive detachment of presynaptic terminals from perikarya and dendrites of axotomized neurons (4)(5)(6)(7)(8)(9)(10)(11)(12). We have previously shown that mRNAs for MHC class I and, in particular, ␤2-microglobulin (␤2m), which is a cosubunit for MHC class I, are up-regulated in spinal motoneurons after peripheral axotomy (13).…”

mentioning

confidence: 99%

A role for MHC class I molecules in synaptic plasticity and regeneration of neurons after axotomy

Oliveira

Thams²,

Lidman³

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

255

232

View full text Add to dashboard Cite

Behavior of neurons in the abducens nucleus of the alert cat—III. Axotomized motoneurons

Cited by 90 publications

References 36 publications

Complementary Actions of BDNF and Neurotrophin-3 on the Firing Patterns and Synaptic Composition of Motoneurons

Complementary Actions of BDNF and Neurotrophin-3 on the Firing Patterns and Synaptic Composition of Motoneurons

Nerve Growth Factor Regulates the Firing Patterns and Synaptic Composition of Motoneurons

A role for MHC class I molecules in synaptic plasticity and regeneration of neurons after axotomy

Contact Info

Product

Resources

About