Behavior of Nucleoli and Contracting Nucleolar Vacuoles in Tobacco Cells Growing in Microculture

Johnson, J. Morris; Jones, Leo E.

doi:10.2307/2440797

Cited by 19 publications

(9 citation statements)

References 11 publications

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“…Examples of patterns of contraction and reformation of nucleolar vacuoles within individual tobacco cells are presented in Fig. 17-19; see Johnson (1964) for additional examples.…”

Section: Results-general Observations On the Nucleolus-mentioning

confidence: 99%

“…The repeated contraction of nucleolar vacuoles in living cultured tobacco cells appeared to be releasing a quantity of some fluid material into the nucleus. For example, in one active cell 6 nucleolar vacuoles with an average volume of 50 IJ.~were seen to form and contract in an 8-hrperiod (Johnson, 1964). The visible opening from the vacuole into the nucleus, the measurable decrease in diameter of the enclosing nucleolar matrix following a vacuolar contraction, the collapse of the matrix into the vacuole in some fixed cells and the inability to see solid material move from the vacuole lend credence to the conclusion that the material in the vacuole is .…”

Section: Results-general Observations On the Nucleolus-mentioning

confidence: 99%

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Behavior of Nucleoli and Contracting Nucleolar Vacuoles in Tobacco Cells Growing in Microculture

Johnson

Jones

1967

American J of Botany

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The ability to observe for extended periods of time individual tobacco cells growing in microculture has made it possible to describe the behavior of their nucleoli and contracting nucleolar vacuoles. Nucleoli typically disappeared in prophase and reappeared in telophase. If several nucleoli were present in telophase they generally fused to form only one or two during interphase. In one instance a nucleolus was seen to separate into two nucleoli prior to disappearance in late prophase. In aging and senescent cells the number of nucleoli or bodies similar to normal nucleoli often increased, and occasionally fragmentation of nucleoli was noted prior to death of cells. Budding of solid material from the nucleolus was also observed. The amount of nucleolar material decreased rapidly prior to death of tobacco cells. Nucleolar vacuoles were found to be a general and consistent component of tobacco cells in microculture. Nucleolar vacuoles typically formed and contracted repeatedly in interphase nuclei and apparently released a fluid material into the nucleus. Associated with the contraction of the nucleolar vacuoles was a corresponding decrease in diameter of the nucleolus. Nucleolar vacuoles were observed to occur in about 70% of the actively growing cells examined, whereas they were present in only 33% of the senescent or weakened cells. These data indicate a relationship between nucleolar vacuoles and the morphogenic status of the cells. Since it has been shown by others that the nucleolus is an active site of RNA metabolism, it is suggested that the contracting nucleolar vacuoles may be involved in the controlled release of a soluble product associated with RNA metabolism.

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“…Examples of patterns of contraction and reformation of nucleolar vacuoles within individual tobacco cells are presented in Fig. 17-19; see Johnson (1964) for additional examples.…”

Section: Results-general Observations On the Nucleolus-mentioning

confidence: 99%

Section: Results-general Observations On the Nucleolus-mentioning

confidence: 99%

Behavior of Nucleoli and Contracting Nucleolar Vacuoles in Tobacco Cells Growing in Microculture

Johnson

Jones

1967

American J of Botany

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“…The occurrence of the ring-shaped nucleolus with a pyroninophilic perimeter coupled with an empty central vacuole is of interest since similar nucleoli have been reported in other cell types such as plant cells, (10,12), nerve cells ( 5 ) , oocytes (3, 6) and lymphocytes (22, 23). T h e similarity in nucleolar morphology suggests that A. galeacystis offers an opportunity to extend studies on the comparative biology of nucleoli to these protozoa.…”

Section: Discussionmentioning

confidence: 70%

Nuclear Division in the Ameboflagellate Adelphamoeba galeacystis

Napolitano

Smith

1975

The Journal of Protozoology

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Nuclear division is synchronized cultures of the ameoboflagellate Adelphamoeba galecystis has been described. Division in this organism is typically promitotic. It occurs within an intact nuclear membrane and is characterized by the persistence of the nucleolus and its transformation into 2 polar masses. The nucleolus is stained with pyronin-Y by the methyl green pyronin-Y technic, and with Heidenhain's hematoxylin, but is unstained by the Feulgen reaction. The reaction with these stsins is removed after digestion of the nucleolus by ribonuclease. During mitosis the nucleolus undergoes an orderly series of vacuolizations before forming the polar masses. The chromatin is Feulgen positive, stains with methyl green by the methyl green pyronin-Y technic and undergoes a series of characteristic changes during the division process. Synchronizationof amebae grown on coverglasses was accomplished by transfer of cells from 30 to 38.5 C for a period of 100 min. A temporal sequence of nucleolar and chromatin participation in the nuclear division of this organism is suggested.

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“…Decrease in nucleolar volume and nucleolar vacuole number as well as nucleolar activity in aging and in weaken tobacco cultured cells were observed (Johnson and Jones 1967). However, in Zea mays root tip cells the number of vacuoles in a nucleolus was inversely related to the nucleolar volume -appearance of nucleolar vacuoles resulted in nucleoli diminution, the smaller nucleoli correlated with the highest vacuolation which in turn depended on the activity of RNA production in a nucleolus (Barlow 1970, De Barsy et al 1974).…”

Section: Discussionmentioning

confidence: 99%

Nucleolar vacuolation in soybean root meristematic cells during recovery after chilling

Stępiński¹

2008

Biologia plant.

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The nucleolar vacuole formation in soybean root meristematic cells from seedlings grown 3 d at temperature 25 °C (control), 3 d at temperature 25 °C and then transferred to 10 °C (chilling) for 4 d, and after recovery for 1.5, 3, 6, 12 and 24 h at 25 °C were observed on semi-thin sections. Simultaneously, autoradiographic studies with 3 H-uridine on squashed preparations were carried out. During recovery of plants, the number of vacuolated nucleoli increased gradually from 24 % after 1.5 h up to 40 % after 24 h, while in the control there were 18 % of nucleoli with vacuoles and after 4-d chilling only 5 %. Labelling of cells during 20-min incubation in 3 H-uridine and during 80-min postincubation in non-radioactive medium was increased in recovered plants in comparison with the control and chilled plants. The conclusion has been drawn that nucleolar vacuoles in soybean plants are formed as a result of migration of granular component accumulated in nucleolus during 4-d chilling.

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Behavior of Nucleoli and Contracting Nucleolar Vacuoles in Tobacco Cells Growing in Microculture

Cited by 19 publications

References 11 publications

Behavior of Nucleoli and Contracting Nucleolar Vacuoles in Tobacco Cells Growing in Microculture

Behavior of Nucleoli and Contracting Nucleolar Vacuoles in Tobacco Cells Growing in Microculture

Nuclear Division in the Ameboflagellate Adelphamoeba galeacystis

Nucleolar vacuolation in soybean root meristematic cells during recovery after chilling

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