Musty odor occurred in tap water with Oita River as its source in October 2014. Oita city disclosed that the problem was due to plankton in the Serikawa Dam, which is on a tributary of the Oita River. This study was aimed at elucidating the cause of the problem by comparing water quality in 2014 with that in previous years. 2-Methylisoborneol (2-MIB) showed a high concentration in the Serikawa Dam and downstream in 2014. It was found that thermal stratifi cation was absent from November 2014 to January 2015 because no temperature difference between the upper and lower layers was detected. On the other hand, the ammonia nitrogen concentration remained high, 2.3 mg•L-, from November 2014 to January 2015. Iron and manganese ions showed the same pattern as ammonia nitrogen in 2014. This suggests that, in 2014, 2-MIB negligibly decomposed as a result of oxygen depletion caused by the high ammonia nitrogen concentration in the Serikawa Dam Reservoir. It is likely that 2-MIB reached farther downstream without being decomposed.