Behavior of osteoblast-like cells on calcium-deficient hydroxyapatite ceramics composed of particles with different shapes and sizes

Kamitakahara, Masanobu; Uno, Yuika; Ioku, Koji

doi:10.1007/s10856-013-5063-6

Cited by 8 publications

(6 citation statements)

References 36 publications

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“…Consequently, high surface roughness restricts cell adhesion and proliferation. Earlier studies also have reported that cell proliferation was suppressed on plate-like morphologies with sharp tips (widths < 1 μm) compared to smoother surfaces without sharp features which attributed to larger contact domains on smoother surfaces [47].…”

Section: Effect Of Surface Morphology On Cell Proliferation Viability and Gene Expressionmentioning

confidence: 82%

Mechanical and biological properties of electrodeposited calcium phosphate coatings

Mokabber

Zhou

Vakis

et al. 2019

Materials Science and Engineering: C

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Calcium phosphate (CaeP) coatings were electrochemically deposited on titanium substrates. By increasing the electrodeposition time (from 1 to 30 min), the coating thickness increases but also the surface morphology of the CaeP coatings is greatly affected going from smooth to plate-like, featuring elongated plates, ribbon-like and finally sharp needle structures. Micro-stretch tests reveal that, regardless of the coating morphology and thickness, the electrodeposited CaeP coatings have strong adhesion with the titanium substrates and their failure mode is cohesive failure. The effects of different morphologies on cellular behavior such as adhesion, viability, proliferation, and osteogenic gene expression were studied. The surface morphology of CaeP coatings has a remarkable effect on cell attachment, proliferation, and viability. A smooth surface results in better adhesion of the cells, whereas the presence of sharp needles and ribbons on rough surfaces restricts cell adhesion and consequently cell proliferation and viability. The improved cell adhesion and viability on the smoother surface can be attributed to the higher contact area between the cell and the coating, while the needle-like morphology inflicts damage to the cells by physically disrupting the cell wall. There is no significant difference in the level of osteoblast gene expression when osteosarcoma cells are cultured on coatings with different morphologies. Our study provides crucial insights into the optimum electrodeposition procedures for CaeP coating formation leading to both good cell-material interaction and sufficient mechanical properties. This can be achieved with relatively thin coatings produced by short electrodeposition times.

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Section: Effect Of Surface Morphology On Cell Proliferation Viability and Gene Expressionmentioning

confidence: 82%

Mechanical and biological properties of electrodeposited calcium phosphate coatings

Mokabber

Zhou

Vakis

et al. 2019

Materials Science and Engineering: C

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“…This behavior may be attributed to the larger contact surface area between the cells and the coating [ 20 , 65 ]. Kamitakahara et al [ 66 ] reported that the wide contact surface area is an important factor for cell proliferation and cells could proliferate easily. Mokabber et al [ 20 ] indicated the smooth surface of calcium phosphate coatings resulted in better proliferation of the cells, whereas the rough calcium phosphate coatings restricted cell adhesion and consequently cell proliferation.…”

Section: Resultsmentioning

confidence: 99%

In-Situ Synthesis and Characterization of Chitosan/Hydroxyapatite Nanocomposite Coatings to Improve the Bioactive Properties of Ti6Al4V Substrates

et al. 2020

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Ti6Al4V alloy is still attracting great interest because of its application as an implant material for hard tissue repair. This research aims to produce and investigate in-situ chitosan/hydroxyapatite (CS/HA) nanocomposite coatings based on different amounts of HA (10, 50 and 60 wt.%) on alkali-treated Ti6Al4V substrate through the sol-gel process to enhance in vitro bioactivity. The influence of different contents of HA on the morphology, contact angle, roughness, adhesion strength, and in vitro bioactivity of the CS/HA coatings was studied. Results confirmed that, with increasing the HA content, the surface morphology of crack-free CS/HA coatings changed for nucleation modification and HA nanocrystals growth, and consequently, the surface roughness of the coatings increased. Furthermore, the bioactivity of the CS/HA nanocomposite coatings enhanced bone-like apatite layer formation on the material surface with increasing HA content. Moreover, CS/HA nanocomposite coatings were biocompatible and, in particular, CS/10 wt.% HA composition significantly promoted human mesenchymal stem cells (hMSCs) proliferation. In particular, these results demonstrate that the treatment strategy used during the bioprocess was able to improve in vitro properties enough to meet the clinical performance. Indeed, it is predicted that the dense and crack-free CS/HA nanocomposite coatings suggest good potential application as dental implants.

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“…Hydroxyapatite (HA) is a major chemical constituent of skeletal bone, and it is converted into Ca 2+ and PO 4 3- in the body ( Couto et al, 2010 ). When utilized in orthopedics, HA promotes bone development and osteointegration, possibly by increasing adhesion and osteoblast proliferation, resulting in faster healing of bone ( Kamitakahara et al, 2014 ). Therefore, HA promotes osteoblast bioactivity to speed-up bone regeneration ( Lin et al, 2009 ; Iafisco et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Estradiol and zinc-doped nano hydroxyapatite as therapeutic agents in the prevention of osteoporosis; oxidative stress status, inflammation, bone turnover, bone mineral density, and histological alterations in ovariectomized rats

et al. 2022

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Osteoporosis (OP) is a serious health problem, and the most popular therapeutic strategy for OP is hormone replacement (estrogen); however, it increases the risk of reproductive cancers. Hydroxyapatite (HA) nanoparticles have a similar chemical structure to the bone mineral component and can be used as a new remedy for OP. This study was designed to investigate the osteoporosis-protective potential of nano zinc hydroxyapatite (ZnHA-NPs) and/or estradiol (E2) combined therapy. A total of 35 adult female rats were assigned into five groups (n = 7): 1) control group; 2) ovariectomized group (OVX); 3) OVX received oral estradiol replacement therapy (OVX/E2); 4) OVX received ZnHA replacement therapy (OVX/ZnHA); and 5) OVX received both estradiol and ZnHA-NPs combined therapy (OVX/E2+ZnHA). After 3 months of treatment, serum bone markers and estrogen level, oxidative/antioxidant, and inflammatory cytokines were determined. Additionally, femoral expression of estrogen receptors alpha and beta (ESR1; ESR2), receptor activator of nuclear factor-kappa B (RANKL) ligand, osteoprotegerin (OPG), bone mineral density (BMD), histological alterations, and immunohistochemical expression of vascular endothelial growth factor (VEGF) and proliferating cell nuclear antigen (PCNA) were assessed. ALP, PINP, Ca, and P concentrations improved significantly (p < 0.05) in all treatment groups, especially in the OVX/E + ZnHA group. MDA and NO were higher in OVX rats, while SOD activity and GSH were lower (p < 0.05). E2 alone or with ZnHA-NPs restored the estimated antioxidant molecules and cytokines toward normal levels in OVX rats (p < 0.05). On the other hand, E2 and ZnHA increased OPG and OC expression in femurs while decreasing ESR1, ESR2, and NF-kB expression (p < 0.05). The combination treatment was superior in the restoration of normal femoral histoarchitecture and both cortical and trabecular BMD (p < 0.05). Overall, the combined therapy of OVX/E2+ZnHA was more effective than the individual treatments in attenuating excessive bone turnover and preventing osteoporosis.

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Behavior of osteoblast-like cells on calcium-deficient hydroxyapatite ceramics composed of particles with different shapes and sizes

Cited by 8 publications

References 36 publications

Mechanical and biological properties of electrodeposited calcium phosphate coatings

Mechanical and biological properties of electrodeposited calcium phosphate coatings

In-Situ Synthesis and Characterization of Chitosan/Hydroxyapatite Nanocomposite Coatings to Improve the Bioactive Properties of Ti6Al4V Substrates

Estradiol and zinc-doped nano hydroxyapatite as therapeutic agents in the prevention of osteoporosis; oxidative stress status, inflammation, bone turnover, bone mineral density, and histological alterations in ovariectomized rats

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