Behavior of period-altered circadian rhythm mutants ofDrosophila in light: Dark cycles (Diptera: Drosophilidae)

Hamblen-Coyle, Melanie J.; Wheeler, David A.; Rutila, Joan E.; Rosbash, Michael; Hall, Jeffrey C.

doi:10.1007/bf01058189

Cited by 165 publications

(143 citation statements)

References 39 publications

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“…1E). The evening peak in activity also arrives earlier during the light phase than the wild-type, possibly suggesting an underlying short-period oscillation, because a similar pattern has been reported in LD conditions for the per s 19-h mutant (19 (Fig. 1G).…”

Section: Resultsmentioning

confidence: 62%

Disruption of Cryptochrome partially restores circadian rhythmicity to the arrhythmic period mutant of Drosophila

Collins

Dissel

Gaten

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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The Drosophila melanogaster circadian clock is generated by interlocked feedback loops, and null mutations in core genes such as period and timeless generate behavioral arrhythmicity in constant darkness. In light-dark cycles, the elevation in locomotor activity that usually anticipates the light on or off signals is severely compromised in these mutants. Light transduction pathways mediated by the rhodopsins and the dedicated circadian blue light photoreceptor cryptochrome are also critical in providing the circadian clock with entraining light signals from the environment. The cry b mutation reduces the light sensitivity of the fly's clock, yet locomotor activity rhythms in constant darkness or light-dark cycles are relatively normal, because the rhodopsins compensate for the lack of cryptochrome function. Remarkably, when we combined a period-null mutation with cry b , circadian rhythmicity in locomotor behavior in light-dark cycles, as measured by a number of different criteria, was restored. This effect was significantly reduced in timeless-null mutant backgrounds. Circadian rhythmicity in constant darkness was not restored, and TIM protein did not exhibit oscillations in level or localize to the nuclei of brain neurons known to be essential for circadian locomotor activity. Therefore, we have uncovered residual rhythmicity in the absence of period gene function that may be mediated by a previously undescribed period-independent role for timeless in the Drosophila circadian pacemaker. Although we do not yet have a molecular correlate for these apparently iconoclastic observations, we provide a systems explanation for these results based on differential sensitivities of subsets of circadian pacemaker neurons to light.anticipation ͉ phase shift ͉ oscillator ͉ hourglass ͉ timeless T he PERIOD (PER) and TIMELESS (TIM) proteins are core components of the negative feedback loop that drives circadian oscillations in Drosophila. TIM stabilizes PER, and the PER and TIM proteins cooperate in nuclear entry and retention in key pacemaker cells at night (1). TIM degrades rapidly in response to light, releasing PER to repress per and tim transcription (1). D. melanogaster individuals carrying the null mutation per 01 display arrhythmic locomotor activity under constant darkness (DD), whereas in light-dark (LD) cycles, their locomotor activity is elevated under illumination and reduced in darkness, reflecting the ''masking'' effect of light (2, 3). Another mutation in the gene encoding the blue-light photoreceptor cryptochrome, cry b , gives attenuated light responses in a circadian context (4-7), but normal circadian locomotor behavior in 24-h LD cycles. As part of another study of seasonal behavioral responses to temperature and light effected via changes in per 3Ј splicing, we generated a per 01 ; cry b double mutant (8). We noted that a modest cycle in wild-type per 3Ј splicing in LD cycles at 29°C was exaggerated in per 01 and cry b mutants, yet in the double mutant, the amplitude of the splicing reverted back to ap...

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Section: Resultsmentioning

confidence: 62%

Disruption of Cryptochrome partially restores circadian rhythmicity to the arrhythmic period mutant of Drosophila

Collins

Dissel

Gaten

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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“…To this end, we examined behavioral rhythms in flies in which dHo expression was knocked down in various cell types by two independent UAS-dHo-RNAi lines by using the binary Gal4-UAS system 22 . Under standard 12 h-12 h light-dark (LD) cycles, wild-type flies exhibit crepuscular behavior, with pronounced morning (M) and evening (E) activity peaks 23 (Fig. 8a).…”

Section: Heme Oxygenase Is Essential For Normal Daily Activity Pattermentioning

confidence: 99%

Reciprocal regulation of carbon monoxide metabolism and the circadian clock

Klemz

Reischl

Wallach

et al. 2016

Nat Struct Mol Biol

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“…In Drosophila and other organisms, this type of behavior is known as "anticipation" and serves as a clear indication for an underlying synchronization of the circadian clock (e.g. Hamblen-Coyle et al, 1992).…”

Section: Observation and Analysis Of Colony Behavior Using Rfidmentioning

confidence: 99%

Daily Changes in Ultraviolet Light Levels Can Synchronize the Circadian Clock of Bumblebees (Bombus terrestris)

Chittka

Stelzer

Stanewsky

2013

Chronobiology International

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Endogenous circadian clocks are synchronized to the 24-h day by external zeitgebers such as daily light and temperature cycles. Bumblebee foragers show diurnal rhythms under daily light:dark cycles and short-period freerunning circadian rhythms in constant light conditions in the laboratory. In contrast, during the continuous light conditions of the arctic summer, they show robust 24-h rhythms in their foraging patterns, meaning that some external zeitgeber must entrain their circadian clocks in the presence of constant light. Although the sun stays above the horizon for weeks during the arctic summer, the light quality, especially in the ultraviolet (UV) range, exhibits pronounced daily changes. Since the photoreceptors and photopigments that synchronize the circadian system of bees are not known, we tested if the circadian clocks of bumblebees (Bombus terrestris) can be entrained by daily cycles in UV light levels. Bumblebee colonies were set up in the laboratory and exposed to 12 h:12 h UV + : UV− cycles in otherwise continuous lighting conditions by placing UV filters on their foraging arenas for 12 h each day. The activity patterns of individual bees were recorded using fully automatic radiofrequency identification (RFID). We found that colonies manipulated in such a way showed synchronized 24-h rhythms, whereas simultaneously tested control colonies with no variation in UV light levels showed free-running rhythms instead. The results of our study show that bumblebee circadian rhythms can indeed be synchronized by daily cycles in ambient light spectral composition. (Author correspondence: r.stanewsky@qmul.ac.uk)

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Behavior of period-altered circadian rhythm mutants ofDrosophila in light: Dark cycles (Diptera: Drosophilidae)

Cited by 165 publications

References 39 publications

Disruption of Cryptochrome partially restores circadian rhythmicity to the arrhythmic period mutant of Drosophila

Disruption of Cryptochrome partially restores circadian rhythmicity to the arrhythmic period mutant of Drosophila

Reciprocal regulation of carbon monoxide metabolism and the circadian clock

Daily Changes in Ultraviolet Light Levels Can Synchronize the Circadian Clock of Bumblebees (Bombus terrestris)

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