Behavior of Staphylococcus aureus and autochthone microbiota in fresh sausages added of sodium nitrite and stored under refrigeration

Abstract

Fresh sausages are cured meat products that may be contaminated with Staphylococcus aureus during the manufacturing procedure, which is frequently related with inadequate handling practices. The use of nitrite in meat products has proven efficacy against Clostridium botulinum, and studies indicate that bactericidal action against S. aureus depends on factors that are intrinsic and extrinsic to the product. The objective of the present study was to evaluate the effect of nitrite concentration, and pH on S. aureus and psychrotrophic autochthone microbiota in fresh sausages stored at different times and temperatures. Fresh sausage were produced at nitrite concentrations 50, 150 and 200ppm and contaminated with S. aureus. The sausages were storage at refrigeration (7 and 12°C) and the quantification of S. aureus and psychrotrophic microorganisms was carried out on days 0, 2, 4, 7, and 10. Results showed that nitrite concentrations and the temperatures used had minimal effect on the multiplication of S. aureus and psychrotrophic autochthone microbiota. Final counts depended only on the length of storage: at the end of 10 days, counts were statistically similar in the different groups, showing that temperature and nitrite concentrations used did not control microbial growth effectively. It is suggested that the product should be stored below 7°C or at freezing temperatures for greater microbiological stability

Relating physicochemical and microbiological safety indicators during processing of linguiça , a Portuguese traditional dry-fermented sausage

“…6). Inoculating S. aureus cells in fresh sausages, Correia, Pereira, Pinto, Barcellos, and Bersot (2014) observed that nitrite at the highest concentration of 200 ppm did not control their growth. Interestingly, the factor analysis revealed some positive association (r = 0.665; Table 3) of nitrites and S. aureus counts during ripening, which, although can be only coincidental or confounding, one cannot dismiss the fact that curing salts may promote the growth of staphylococci under anoxic conditions (in the core of the sausages) as a function of their nitrite-and nitrate-reducing systems.…”

Grau de cumprimento das boas práticas de fabricação e perfil microbiológico de linguiças frescas mistas

“…A linguiça é o produto cárneo mais produzido no Brasil, feita de carne bovina, suína ou de aves, podendo ou não ser salgada, maturada, seca ou cozida (8) . Dentre os tipos de linguiça, a linguiça fresca se destaca pela grande aceitação entre os consumidores (8)(9)(10) .…”

Level of compliance with good manufacturing practices and microbiological profile of mixed fresh sausages

“…Sausage is the most produced meat product in Brazil, made from beef, pork or poultry, and may or may not be salted, aged, dried or cooked (8) . Among the types of sausage, fresh sausage stands out due to its great acceptance among consumers (8)(9)(10) .…”