Behavior of the Edible Seaweed Sargassum fusiforme to Copper Pollution: Short-Term Acclimation and Long-Term Adaptation

Zou, Huixi; Pang, Qiuying; Lin, Lidong; Zhang, Aiqin; Li, Nan; Lin, Yanqing; Li, Lu-Min; Wu, Qin

doi:10.1371/journal.pone.0101960

Cited by 18 publications

(13 citation statements)

References 77 publications

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“…), green alga Chlamydomonas reinhardtii (Chen and Silflow ), and macroalga Sargassum fusiforme (Zou et al. ). All the N taken up by plants is almost assimilated into glutamine, with a small amount to asparagine (Lee et al.…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, the transcription and enzyme activity of gs gene in cyanobacterium Synechococcus sp., were also strongly induced under N starvation (Sauer et al 2000). Besides N deficiency, different N forms (e.g., ammonium and nitrate) and stresses are also important in the expression of gs genes, such as the studies in diatom Skeletonema costatum (Takabayashi et al 2005), green alga Chlamydomonas reinhardtii (Chen and Silflow 1996), and macroalga Sargassum fusiforme (Zou et al 2014). All the N taken up by plants is almost assimilated into glutamine, with a small amount to asparagine (Lee et al 2018).…”

Section: Effects Of Nitrogen On Gracilariopsis Lemaneiformismentioning

confidence: 99%

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Physiological effects of nitrogen deficiency and recovery on the macroalga Gracilariopsis lemaneiformis (Rhodophyta)

Liu

Wen

Chen

et al. 2019

Journal of Phycology

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Algal metabolites are the most promising feedstocks for bio‐energy production. Gracilariopsis lemaneiformis seems to be a good candidate red alga for polysaccharide production, especially relating to the agar production industry. Nitrogen deficiency is an efficient environmental pressure used to increase the accumulation of metabolites in algae. However, there are no studies on the physiological effects of G. lemaneiformis in response to nitrogen deficiency and its subsequent recovery. Here we integrated physiological data with molecular studies to explore the response strategy of G. lemaneiformis under nitrogen deficiency and recovery. Physiological measurements indicated that amino acids and protein biosynthesis were decreased, while endogenous NH4+ and soluble polysaccharides levels were increased under nitrogen stress. The expression of key genes involved in these pathways further suggested that G. lemaneiformis responded to nitrogen stress through up‐regulation or down‐regulation of genes related to nitrogen metabolism, and increased levels of endogenous NH4+ to complement the deficiency of exogenous nitrogen. Consistent with the highest accumulation of soluble polysaccharides, the gene encoding UDP‐glucose pyrophosphorylase, a molecular marker used to evaluate agar content, was dramatically up‐regulated more than 4‐fold compared to the relative expression of actin after 4 d of nitrogen recovery. The present data provide important information on the mechanisms of nutrient balance in macroalgae.

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Section: Discussionmentioning

confidence: 99%

Section: Effects Of Nitrogen On Gracilariopsis Lemaneiformismentioning

confidence: 99%

Physiological effects of nitrogen deficiency and recovery on the macroalga Gracilariopsis lemaneiformis (Rhodophyta)

Liu

Wen

Chen

et al. 2019

Journal of Phycology

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“…This metabolite, also described as the major component of S. fusiforme tissue extracts, is one of the main products of brown algal photosynthesis and may act as a radical scavenger to detoxify oxidative stress (Zou et al. 2014). Additional abundant resonances mainly in the carbinolic and aliphatic regions of the spectra can be attributed to sugar and amino acid protons.…”

Section: Resultsmentioning

confidence: 99%

NMR Metabolic Profiling of Sargassum Species Under Different Stabilization/Extraction Processes

Hernández-Bolio¹,

Fagundo-Mollineda²,

Caamal-Fuentes³

et al. 2021

Journal of Phycology

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The genus Sargassum is well represented by benthic and pelagic species, some of which form massive aggregates that can travel long distances due to the force of the ocean currents. Although they constitute an essential habitat for fish and invertebrate species, large accumulations of Sargassum in coastal areas generate several economic, environmental, and health impacts. It is important to recognize the species forming these aggregates, and identify the metabolites they produce, allowing for its exploitation, and therefore, better management practices. NMR metabolic profiling is a technique that can discriminate samples while detecting their unique or differential chemical features, and has been successfully used in the study and classification of several algal species. The present investigation studied the metabolic profiling of Sargassum species found on strandings at Puerto Morelos (Quintana Roo) east coast of the Mexican Caribbean. PCA of the 1H‐NMR profiles corresponding to S. natans, S. natans (morphotype VIII), S. fluitans, and a benthic Sargassum buxifolium allowed the discrimination of samples amongst them. Furthermore, discrimination between the two forms of S. natans was also possible. The PCA loading plot revealed that glutamine and glutamate have the highest influence in the clustering of the benthic Sargassum, while a high abundance of lactate, Myo‐inositol, and trimethylamine is a unique feature from the S. natans morphotype VIII. Additional PLS‐DA models showed that a heat‐drying process improved the extraction of metabolites. Maceration and microwave‐assisted extraction with water‐ethanol led to similar profiles and thus any of them could be used in future investigations.

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“…These species-specific variations are probably related to sensitivity to chemical interaction and biosorption capacity. It is expected that each species will have different availability of chemical binding sites which, in turn, could facilitate incorporation of metals to the intracellular matrix, triggering the process of biochemical defence or inducing mobility to specific regions of the cell in response to chemicals such as Cd (Zou et al 2014;Zhao et al 2015). Considering the chronic effect at low concentrations of metal, we supposed that this seaweed could tolerate these exposure limits, expressing ultrastructural and biochemical adjustments as an acclimation strategy to these oxidative stressful conditions.…”

Section: Discussionmentioning

confidence: 99%

The brown seaweed Sargassum cymosum: changes in metabolism and cellular organization after long-term exposure to cadmium

et al. 2016

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Sargassum cymosum was exposed to cadmium (Cd) to determine any physiological and ultrastructural effects. To accomplish this, S. cymosum samples were cultivated under photosynthetic active radiation (PAR) and Cd (0, 0.1, 0.2, 0.4 and 0.8 mg L) during 7 and 14 days in laboratory-controlled conditions (0 mg L Cd at both exposure times as control). Seaweeds had high retention capacity (over 90 %) for both exposure times. Growth rates showed significant increases by 14 days, especially for 0.1 and 0.4 mg L Cd. Photosynthetic parameters were unaffected by Cd treatments. Chlorophyll contents were present in higher concentrations for all Cd treatments compared to respective control. Carotenoid profile showed significant differences in total composition and proportion of fucoxanthin and β-carotene, and no lutein was detected at 14 days. Phenolic and flavonoid compounds showed major accumulation at 14 days. Transmission electron microscopy (TEM) analyses presented major alterations in Cd-treated samples, when compared with respective control, in particular disorganization of cell wall fibrils. When compared to respective control samples, multivariate analyses showed disparate and complex interactions among metabolites in Cd-exposed seaweeds, giving evidence of physiological defence response. Thus, it can be concluded that Cd is a stressor for S. cymosum, resulting in physiological and structural alterations related to defence mechanisms against oxidative stress and toxicological effects resulting from long-term metal exposure. However, in the present paper, some observed changes also appear to result from acclimation mechanisms under lower concentration of Cd relative to the tolerance of S. cymosum to experimental conditions.

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Behavior of the Edible Seaweed Sargassum fusiforme to Copper Pollution: Short-Term Acclimation and Long-Term Adaptation

Cited by 18 publications

References 77 publications

Physiological effects of nitrogen deficiency and recovery on the macroalga Gracilariopsis lemaneiformis (Rhodophyta)

Physiological effects of nitrogen deficiency and recovery on the macroalga Gracilariopsis lemaneiformis (Rhodophyta)

NMR Metabolic Profiling of Sargassum Species Under Different Stabilization/Extraction Processes

The brown seaweed Sargassum cymosum: changes in metabolism and cellular organization after long-term exposure to cadmium

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