“…This study showed that PEF treatment did not affect negatively the total number of microorganisms which oscillated between 2.9•10 9 and 1.29•10 11 cfu/mL. Seratlic et al [40] also observed that electroporation of L. plantarum 564 cells at a field strength less than 13.6 kV/cm does not reduce the total number of microorganisms.…”
Section: Optimization Of Zinc Concentration In Culture Medium and Pefmentioning
Pulsed electric field (PEF) technology was used to enrich Lactobacillus rhamnosus B 442 cells in zinc ions to obtain source of this element with high bioavailability. The highest bioaccumulation of zinc was achieved when electroporation was performed at optimal parameters: field strength of 3.0 kV/cm, pulse width of 20 µs, electroporation time of 15 min after 20 h of culturing and at zinc concentration of 500 µg/mL medium. The maximum bioaccumulation of ions in cells (2.85 mg Zn/g d.m.) was 164% higher than in the control sample which was supplemented with zinc but not treated with PEF. The action of PEF did not reduce the total number of microorganisms in the medium or the biomass of bacteria.
“…This study showed that PEF treatment did not affect negatively the total number of microorganisms which oscillated between 2.9•10 9 and 1.29•10 11 cfu/mL. Seratlic et al [40] also observed that electroporation of L. plantarum 564 cells at a field strength less than 13.6 kV/cm does not reduce the total number of microorganisms.…”
Section: Optimization Of Zinc Concentration In Culture Medium and Pefmentioning
Pulsed electric field (PEF) technology was used to enrich Lactobacillus rhamnosus B 442 cells in zinc ions to obtain source of this element with high bioavailability. The highest bioaccumulation of zinc was achieved when electroporation was performed at optimal parameters: field strength of 3.0 kV/cm, pulse width of 20 µs, electroporation time of 15 min after 20 h of culturing and at zinc concentration of 500 µg/mL medium. The maximum bioaccumulation of ions in cells (2.85 mg Zn/g d.m.) was 164% higher than in the control sample which was supplemented with zinc but not treated with PEF. The action of PEF did not reduce the total number of microorganisms in the medium or the biomass of bacteria.
“…The prolonged lag growth phase was also detected, which is another characteristic of persisters (Seratli c et al 2013). plantarum 564 cells were 9.39 log cfu/mL, while the number of spray-dried cells was 10.23 log cfu/g.…”
Section: Results and Discussion Cell Enumeration Of Lb Plantarum 564mentioning
confidence: 87%
“…plantarum 564 cells resumed higher growth rate than the untreated cells, indicating the presence of bacterial persistence after the exposure to induced stress. The prolonged lag growth phase was also detected, which is another characteristic of persisters (Seratli c et al 2013). During this phase, an increase in the biosynthesis of enzymes involved in the formation of phospholipids responsible for cell membrane strengthening occurs, so the cell is being prepared for survival under stress conditions (Cohen et al 2006).…”
Section: Results and Discussion Cell Enumeration Of Lb Plantarum 564mentioning
A high viability of probiotics in food product, with a living cells threshold of 10 /cfu/g (colony-forming units/g) is a challenge to achieve in food production. Spray drying is an efficient and economic industrial method for probiotic bacterial preservation and its application in food products. In this study, the survival of free and spray-dried cells of potential probiotic strain Lactobacillus plantarum 564 after production and during 8 weeks of storage of soft acid coagulated goat cheese was investigated, as well as compositional and sensory quality of cheese. Total bacterial count of spray-dried Lb. plantarum 564 cells were maintained at the high level of 8.82 log/cfu/g in cheese after 8 weeks of storage, while free-cell number decreased to 6.9 log/cfu/g. However, the chemical composition, pH values and sensory evaluation between control cheese (C1 sample made with commercial starter culture) and treated cheese samples (C2 and C3, made with the same starter, with the addition of free and spray-dried Lb. plantarum 564 cells, respectively) did not significantly differ. High viability of potential probiotic bacteria and acceptable sensory properties indicate that spray-dried Lb. plantarum 564 strain could be successfully used in the production of soft acid coagulated goat cheeses.
“…in comparison to the culture not supplemented with this element. Seratlić et al ( 2013 ) who studied survivability of Lactobacillus plantarum 564 population treated with PEF showed that bacteria subjected to a multiple electroporation demonstrated higher resistance to further PEF application. The researchers applied electroporation after 3 h from completion of 3-day incubation of the culture at electric field strength of 22.9 and 31.6 kV/cm, pulse width of 5 μs and period between pulses of 10 ms. Fig.…”
The aim of this study was to determine the effect of pulsed electric fields (PEF) on accumulation of magnesium ions in Lactobacillus rhamnosus B 442 cells. Under optimized conditions, this is, on 15 min exposure of the 20 h grown culture to PEF of the 2.0 kV/cm and 20 µs pulse width at concentration 400 μg Mg2+/mL medium, accumulation of magnesium in the biomass reached maximum 4.28 mg/g d.m. Optimization of PEF parameters caused an increase of magnesium concentration in the cells by 220% in comparison to the control not treated with PEF. Bacterial cell biomass enriched with Mg2+ may be an alternative for pharmacological supplementation applied in deficiency of this cation.
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