Glue laminated bamboo (glubam) is a type of engineered bamboo material developed for applications in building structures and interiors. This paper focuses on the fungal (Aspergillus niger) colonization from 14 to 56 days in thick- and thin-strip glubam board with investigation of physical, mechanical (compression), and microcosmic properties. Two-degree of carbonization treatment was employed to improve the antifungal property of the thick-strip glubam. After 56 days of infection, the deep-degree carbonized thick-strip glubam presents better anti-mold properties than medium and non-carbonized specimens. For thin-strip glubam, both parallel and perpendicular to the main bamboo fiber direction were considered. The longitudinal thin-strip glubam retains decent compressive properties, while the transverse specimens stay a stable compressive strength along all fungal tests. The paper reports the experimental values of mass loss, color changes, compressive strengths, modulus of elasticity in compression, and microstructure observations from optical and SEM microscopy at different fungal exposure timespans.