Behavior of vascular resistance undergoing various pressure insufflation and perfusion on decellularized lungs

Palma, Renata Kelly da; Nonaka, Paula Naomi; Campillo, Noelia; Uriarte, Juan J.; Urbano, Jéssica Julioti; Navajas, Daniel; Farré, Ramón; Oliveira, Luís Vicente Franco de

doi:10.1016/j.jbiomech.2016.02.043

Cited by 16 publications

(14 citation statements)

References 11 publications

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“… 13 – 16 , 23 The approach frequently utilized is detergent-based decellularization including SDS, Triton X100, sodium deoxycholate (SDC), and 3-((3-cholamidopropyl)dimethylammonio)-1-propanesulfonate (CHAPS). In this work, we based our lung decellularization process in previous studies using SDS 1%, 23 , 30 – 32 which is a cheap detergent and more efficient for removing cell residue tissue and increased ECM retention when compared to other detergents. 33 , 34 Given that it resulted in a residual DNA level less than 50 ng of DNA per 1 mg of dry weight of the ECM scaffold (19.8 ng/mg), the protocol employed for equine lung decellularization seems suitable.…”

Section: Discussionmentioning

confidence: 99%

Equine lung decellularization: a potential approach for in vitro modeling the role of the extracellular matrix in asthma

et al. 2018

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Contrary to conventional research animals, horses naturally develop asthma, a disease in which the extracellular matrix of the lung plays a significant role. Hence, the horse lung extracellular matrix appears to be an ideal candidate model for in vitro studying the mechanisms and potential treatments for asthma. However, so far, such model to study cell–extracellular matrix interactions in asthma has not been developed. The aim of this study was to establish a protocol for equine lung decellularization that maintains the architecture of the extracellular matrix and could be used in the future as an in vitro model for therapeutic treatment in asthma. For this the equine lungs were decellularized by sodium dodecyl sulfate detergent perfusion at constant gravitational pressure of 30 cmH2O. Lung scaffolds were assessed by immunohistochemistry (collagen I, III, IV, laminin, and fibronectin), scanning electron microscopy, and DNA quantification. Their mechanical property was assessed by measuring lung compliance using the super-syringe technique. The optimized protocol of lung equine decellularization was effective to remove cells (19.8 ng/mg) and to preserve collagen I, III, IV, laminin, and fibronectin. Moreover, scanning electron microscopy analysis demonstrated maintained microscopic lung structures. The decellularized lungs presented lower compliance compared to native lung. In conclusion we described a reproducible decellularization protocol that can produce an acellular equine lung feasible for the future development of novel treatment strategies in asthma.

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Section: Discussionmentioning

confidence: 99%

Equine lung decellularization: a potential approach for in vitro modeling the role of the extracellular matrix in asthma

et al. 2018

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“…In several studies from our laboratory, we perfused the SDS detergent through the trachea [12,15,16] and pulmonary artery [10,13,14], which resulted in an acellular lung with retention of specific ECM components and native cell population removal, which determines an optimal decellularization process. In this study we used the same protocol that was previously applied to lung decellularization with SDS through the trachea [12] and pulmonary artery [13].…”

Section: Discussionmentioning

confidence: 99%

“…The following decellularizing steps were followed through the pulmonary artery: 1) PBS 1× for 30 min, 2) deionized water for 15 min, 3) 1% SDS for 150 min, and 4) PBS for 30 min, at a pressure of 20 cm H 2 O [13,14]. …”

Section: Methodsmentioning

confidence: 99%

Effects of two different decellularization routes on the mechanical properties of decellularized lungs

Urbano¹,

Palma²,

Lima³

et al. 2017

PLoS ONE

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Considering the limited number of available lung donors, lung bioengineering using whole lung scaffolds has been proposed as an alternative approach to obtain lungs suitable for transplantation. However, some decellularization protocols can cause alterations on the structure, composition, or mechanical properties of the lung extracellular matrix. Therefore, the aim of this study was to compare the acellular lung mechanical properties when using two different routes through the trachea and pulmonary artery for the decellularization process. This study was performed by using the lungs excised from 30 healthy male C57BL/6 mice, which were divided into 3 groups: tracheal decellularization (TDG), perfusion decellularization (PDG), and control groups (CG). Both decellularized groups were subjected to decellularization protocol with a solution of 1% sodium dodecyl sulfate. The behaviour of mechanical properties of the acellular lungs was measured after decellularization process. Static (Est) and dynamic (Edyn) elastances were obtained by the end-inspiratory occlusion method. TDG and PDG showed reduced Est and Edyn elastances after lung decellularization. Scanning electron microscopy showed no structural changes after lung decellularization of the TDG and PDG. In conclusion, was demonstrated that there is no significant difference in the behaviour of mechanical properties and extracellular matrix of the decellularized lungs by using two different routes through the trachea and pulmonary artery.

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“…Moreover, exercise increases the mitochondrial biogenesis, fatty acid oxidation and the dilation of blood vessels, that results in improved myocardial perfusion and lower inflammation, reducing as such the risk for atherosclerosis [57]. In particular, regular exercise offers indirect antioxidant protection by enhancing the activity of the endogenously produced antioxidant enzymes [58][59][60]. It should be highlighted though, that exercise induces intensity-, duration-and type-dependent effects on antioxidant mechanisms and oxidative stress levels.…”

Section: Antioxidants Oxidative Stress and Cardiovascular Diseasementioning

confidence: 99%

Exercise-Induced Regulation of Redox Status in Cardiovascular Diseases: The Role of Exercise Training and Detraining

et al. 2019

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Although low levels of reactive oxygen species (ROS) are beneficial for the organism ensuring normal cell and vascular function, the overproduction of ROS and increased oxidative stress levels play a significant role in the onset and progression of cardiovascular diseases (CVDs). This paper aims at providing a thorough review of the available literature investigating the effects of acute and chronic exercise training and detraining on redox regulation, in the context of CVDs. An acute bout of either cardiovascular or resistance exercise training induces a transient oxidative stress and inflammatory response accompanied by reduced antioxidant capacity and enhanced oxidative damage. There is evidence showing that these responses to exercise are proportional to exercise intensity and inversely related to an individual's physical conditioning status. However, when chronically performed, both types of exercise amplify the antioxidant defense mechanism, reduce oxidative stress and preserve redox status. On the other hand, detraining results in maladaptations within a time-frame that depends on the exercise training intensity and mode, as high-intensity training is superior to low-intensity and resistance training is superior to cardiovascular training in preserving exercise-induced adaptations during detraining periods. Collectively, these findings suggest that exercise training, either cardiovascular or resistance or even a combination of them, is a promising, safe and efficient tool in the prevention and treatment of CVDs.

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Behavior of vascular resistance undergoing various pressure insufflation and perfusion on decellularized lungs

Cited by 16 publications

References 11 publications

Equine lung decellularization: a potential approach for in vitro modeling the role of the extracellular matrix in asthma

Equine lung decellularization: a potential approach for in vitro modeling the role of the extracellular matrix in asthma

Effects of two different decellularization routes on the mechanical properties of decellularized lungs

Exercise-Induced Regulation of Redox Status in Cardiovascular Diseases: The Role of Exercise Training and Detraining

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