Abstract-The toxic effect of the insecticide malathion on oxygen consumption and some biochemical characteristics (total protein, carbohydrate and cholesterol in liver, muscle, kidney and gills) of the tilapia fish (oreochromis niloticus) were estimated. The data shows that the rate of oxygen consumption was declined during all the exposure periods. On the other hand, all biochemical's parameters were found to be decreased in all tissues on comparison with control. The results indicated the toxic nature of the insecticide malathion.