Behavioral and biochemical studies of total furocoumarins from seeds of Psoralea corylifolia in the forced swimming test in mice

Chen, Yan; Kong, Ling-Dong; Xia, Xueqi; Kung, Hsiang‐Fu; Zhang, Liang

doi:10.1016/j.jep.2004.09.033

Cited by 75 publications

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References 45 publications

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“…4,5) Successful antidepressant treatment could attenuate these alterations in clinical trials and animal experiments. [6][7][8][9] In addition, there is a close relationship between the serotonergic and HPA axis systems in major depression. [10][11][12][13] The impact of stress on the HPA axis is reviewed and evidence present that CRF is the stress neurotransmitter which plays an important role in the activation of serotonergic systems.…”

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“…Our previous studies demonstrated that the total furocoumarins extracts of P. corylifolia possessed the potent antidepressant properties by employing the forced swimming test (FST), a well-established animal model of depression. 7) These furocoumarins extracts depressed the hyperactive HPA axis induced by swim stress in mice. 7) Furthermore, the total furocoumarins extracts and psoralen exhibited inhibitory actions on monoamine oxidase (MAO) A and B activities.…”

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“…7) These furocoumarins extracts depressed the hyperactive HPA axis induced by swim stress in mice. 7) Furthermore, the total furocoumarins extracts and psoralen exhibited inhibitory actions on monoamine oxidase (MAO) A and B activities. 16,17) Per-orally administered psoralen increased the daytime levels of melatonin via the competitive inhibition of hepatic melatonin metabolism, indicating that psoralen might affect seasonal affective disorder patients under a low level of illumination.…”

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Antidepressant-Like Effects of Psoralen Isolated from the Seeds of Psoralea corylifolia in the Mouse Forced Swimming Test

Pan

et al. 2008

Biological & Pharmaceutical Bulletin

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Depression is associated with behavioral disturbances and serotonergic and neuroendocrinological disfunctions.1,2) The brain serotonergic system is a very complicated control system playing an important role in the response to depression. 3)A number of factors have been proposed to account for a reduced functionality of serotonergic system in depression. Investigations based on this system have formed into currently available antidepressants.2) On the other hand, neuroendocrinological abnormality in depressed patients is hyperactivity of the hypothalamic-pituitary-adrenal (HPA) axis characterized by hypersecretion of corticotropin-releasing factor (CRF), which stimulates corticosterone release. 4,5) Successful antidepressant treatment could attenuate these alterations in clinical trials and animal experiments. [6][7][8][9] In addition, there is a close relationship between the serotonergic and HPA axis systems in major depression. [10][11][12][13] The impact of stress on the HPA axis is reviewed and evidence present that CRF is the stress neurotransmitter which plays an important role in the activation of serotonergic systems. 11,14) Psoralen (Fig. 1) is a main furocoumarin isolated from the seeds of Psoralea corylifolia, 15) which is a traditional Chinese medicine used to treat a wide range of diseases and conditions. Our previous studies demonstrated that the total furocoumarins extracts of P. corylifolia possessed the potent antidepressant properties by employing the forced swimming test (FST), a well-established animal model of depression. 7)These furocoumarins extracts depressed the hyperactive HPA axis induced by swim stress in mice.7) Furthermore, the total furocoumarins extracts and psoralen exhibited inhibitory actions on monoamine oxidase (MAO) A and B activities. 16,17) Per-orally administered psoralen increased the daytime levels of melatonin via the competitive inhibition of hepatic melatonin metabolism, indicating that psoralen might affect seasonal affective disorder patients under a low level of illumination.18) However, there is no study on antidepressant-like effects and related possible mechanisms of actions by psoralen. The aim of the current study was to investigate the effects of psoralen on the durations of immobility, climbing and swimming in the mouse FST after administrations for 1, 3, 7 and 14 d, respectively. We also examined the effects of 14-d psoralen treatment on serotonergic neurotransmitters serotonin (5-HT) and its metabolite 5-hydroxyindoleacetic acid (5-HIAA) levels in frontal cortex and hippocampus of the mouse brain regions. The regulation of psoralen on the HPA axis including CRF and corticosterone concentrations was simultaneously studied in the mouse FST. MATERIALS AND METHODS MaterialsPsoralen was isolated and purified from the seeds of P. corylifolia as described in our previous reports. 19)The purity of the chemical was determined to beyond 98% by HPLC analysis. Amitriptyline was obtained from Changzhou Siyao Pharmaceuticals Co., Ltd. (P. R. China). All other chemical...

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Antidepressant-Like Effects of Psoralen Isolated from the Seeds of Psoralea corylifolia in the Mouse Forced Swimming Test

Pan

et al. 2008

Biological & Pharmaceutical Bulletin

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“…In recent years, many studies focused on analyses of its quality, [1][2][3] phytochemistry, 4,5) and bioactivities. [6][7][8][9][10][11][12][13][14][15][16][17][18] Our liquid chromatography mass spectroscopy (LC-MS) analyses on the chemical profile of the fruits led to the discoveries of two benzofuran glycosides. They were isolated and identified to be the glucosides of psoralen and isopsoralen by detailed spectral analyses, respectively, along with nine known compounds.…”

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Psoralenoside and Isopsoralenoside, Two New Benzofuran Glycosides from Psoralea corylifolia

Qiao

Han

et al. 2006

Chem. Pharm. Bull.

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The fruits of Psoralea corylifolia L. (Fabaceae), wellknown as a traditional Chinese medicine "Buguzhi", are widely applied for the cure of gynaecological bleeding, vitiligo and psoriasis. In recent years, many studies focused on analyses of its quality, 1-3) phytochemistry, 4,5) and bioactivities.6-18) Our liquid chromatography mass spectroscopy (LC-MS) analyses on the chemical profile of the fruits led to the discoveries of two benzofuran glycosides. They were isolated and identified to be the glucosides of psoralen and isopsoralen by detailed spectral analyses, respectively, along with nine known compounds.In the MeOH extract of the fruits of Psoralea corylifolia, two interesting compounds (1, 2) were found by high performance liquid chromatography (HPLC) analyses. They were shown as major peaks in the HPLC chromatogram, but had never been reported. Moreover, they were absent in the boiled water extract of the fruits, but their polarity was close to sugar. The high performance liquid chromatography electrospray ionization mass spectroscopy (HPLC-ESI-MS) analyses showed that they presented the same MS spectra, in which three major ion peaks were displayed clearly. As shown in Fig. 1 Two new benzofuran glycosides, called psoralenoside and isopsoralenoside, were isolated from the fruits of Psoralea corylifolia, together with nine known compounds. Their structures were elucidated by detailed spectral analyses including extensive two dimensional (2D) NMR spectra.

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“…Last year, over ten articles have been published with focus on its quality analyses, [1][2][3] phytochemistry, [4][5][6][7][8] and bioactivities. [9][10][11][12][13][14][15][16] Our previous phytochemical investigation on this medicinal herb revealed two new benzofuran glycosides, psoralenoside (1) and isopsoralenoside (2), using high performance liquid chromatography mass spectroscopy (HPLC-MS) technique. 17) They were found to have interesting structures which were related to the major constituents of Fructus Psoraleae, psoralen (3) and isopsoralen (4).…”

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Quality Assessment of Fructus Psoraleae

Qiao

Han

Song

et al. 2006

CHEMICAL & PHARMACEUTICAL BULLETIN

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Fructus Psoraleae, the dried fruits of Psoralea corylifolia L., is one of the most popular traditional Chinese medicine and is officially listed in the Chinese Pharmacopoeia. This crude drug has been used for the treatment of enuresis, pollakiuria, weak kidney, and pain and cold in the waist and knees. It always attracts research interest from various related fields. Last year, over ten articles have been published with focus on its quality analyses, [1][2][3] phytochemistry, [4][5][6][7][8] and bioactivities.9-16) Our previous phytochemical investigation on this medicinal herb revealed two new benzofuran glycosides, psoralenoside (1) and isopsoralenoside (2), using high performance liquid chromatography mass spectroscopy (HPLC-MS) technique. 17) They were found to have interesting structures which were related to the major constituents of Fructus Psoraleae, psoralen (3) and isopsoralen (4). From the chemistry point of view, psoralen and isopsoralen could be the products of the hydrolyzation of these two new benzofuran glycosides. However, all the previously reported quality analyses of this important herbal medicine were focused on coumarins (3) and (4). Therefore, we carried out a new quality research using both glycosides (1) and (2) and coumarins (3) and (4) as the quality markers, which led to the discovery of some attractive relationships between these chemical markers. ExperimentalReagents and Materials Twenty-three batches of the raw material of Fructus Psoraleae were collected from different regions of China and they were authenticated as genuine P. corylifolia according to their morphological characteristics. The voucher specimens were deposited in the Chinese Medicine Laboratory of Hong Kong Jockey Club Institute of Chinese Medicine. The reference chemical standards of psoralen (batch No. 110739-200309) and isopsoralen (batch No. 110738-200410) were purchased from the National Institute for the Control of Pharmaceutical and Biological Products of China (Beijing, China). Psoralenoside and isopsoralenoside were isolated and purified as described in our previous report.17) The purities of the chemical references were determined to be beyond 98% by HPLC analysis. HPLC grade methanol and acetic acid were purchased from International Laboratory (Nevada, U.S.A.). HPLC grade water was prepared by Millipore Milli-Q SP water purification system. Apparatus and ColumnThe Agilent 1100 series HPLC system equipped with a Zorbax ® XDB-C 8 analytical column (4.6ϫ150 mm, 5 mm, Agilent Technologies, U.S.A.), a C 18 guard column (4.6ϫ12.5 mm, 5 mm, Agilent Technologies, U.S.A.) and a photodiode array detector (DAD) was set up for the analysis. The analysis was performed at 20°C during the whole process. The mobile phase was a mixture of methanol and water (containing 0.1% acetic acid) at a flow rate of 1.0 ml/min. Linear gradient elution from 25 to 60% methanol (v/v) in 30 min was applied. The detection wavelength was set at 246 nm.Standard Solutions The stock solutions were prepared in methanol at the concentrations ...

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Behavioral and biochemical studies of total furocoumarins from seeds of Psoralea corylifolia in the forced swimming test in mice

Cited by 75 publications

References 45 publications

Antidepressant-Like Effects of Psoralen Isolated from the Seeds of Psoralea corylifolia in the Mouse Forced Swimming Test

Antidepressant-Like Effects of Psoralen Isolated from the Seeds of Psoralea corylifolia in the Mouse Forced Swimming Test

Psoralenoside and Isopsoralenoside, Two New Benzofuran Glycosides from Psoralea corylifolia

Quality Assessment of Fructus Psoraleae

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