Behavioral and physiological effects of acute and chronic kava exposure in adult zebrafish

Modulation of behavioral and neurochemical responses of adult zebrafish by fluoxetine, eicosapentaenoic acid and lipopolysaccharide in the prolonged chronic unpredictable stress model

“…One day after the last behavioral experiment (shoaling test on Day 81 of PUCS protocol), the fish (n = 12 per group) were sacrificed in ice water followed by decapitation, their brains dissected on ice, frozen in liquid nitrogen, and stored at − 80 °C. Monoamines in adult zebrafish brain were assayed using the high-performance liquid chromatography (HPLC) 109 , 110 , and were chosen here as key stress biomarkers in various mental disorders in humans, as well as in rodent and zebrafish models 55 , 111 . The study examined norepinephrine, as well as serotonin, dopamine and their respective metabolites 5-HIAA and DOPAC.…”

Understanding complex dynamics of behavioral, neurochemical and transcriptomic changes induced by prolonged chronic unpredictable stress in zebrafish

“…Qualitative real-time polymerase chain reaction (qRT-PCR). To reconfirm the validity of our RNAseq data, we also conducted a small qualitative real-time PCR study of selected genes, quantifying wholebrain expression of four 'reference' genes (isg15, saga, otx5 and tpm4b) and one housekeeping gene (b-act) using the established qrt-PCR protocol 48,119,122,123 , with minor modifications. The genes were chosen based on their hubness in core PPI groups and on different expression patterns between groups (isg15-differentially expressed in both stress and fluoxetine vs. control, saga-in stress vs. fluoxetine, otx5-stress vs. both control and fluoxetine and tpm4b-in stress vs. control).…”

“…Neurochemical analyses. Brain monoamines are an important factor in stress and affective disorders both clinically and in rodents and zebrafish models 37,68,[117][118][119][120][121] . To study dynamic changes in their levels in stressed zebrafish, the whole-brain concentrations of norepinephrine (NE), serotonin (5-HT), dopamine (DA) and their metabolites 5-HIAA, DOPAC and homovanillic acid (HVA) were assayed using the high-performance liquid chromatography (HPLC), as in 68,[119][120][121] .…”

“…6), we performed Kruskal-Wallis (KW) test with post-hoc Dunn's test for pairwise comparison for significant KW data (see Supplementary Table S10 for details). Analyses of all data in this study were performed offline without blinding the analysts to the treatments, since all animals and samples were included in analyses, data were analyzed in a fully unbiased automated method, and the analysts had no ability to influence the results of the experiments, as in 119 . The study experimental design and its description here, as well as data analyses and presenting, adhered to the ARRIVE guidelines for reporting animal research and the PREPARE guidelines for planning animal research and testing.…”

Toxicological and Behavior Analyses of a Foliose Lichen Parmotrema Tinctorum (Delise Ex Nyl.) Hale Extract Using Zebrafish (Danio Rerio) Model

“…of genomic DNA, 5 µl of 25-fold diluted cDNA template mixed with 2.5 µl of each primer and 12.5 µl of EMERALD PCR master mix to a final volume of 25 µl. Amplification of DNA was carried out in 34 cycles using a Thermal cycler (Lark L125, Chennai, India) and the resulting PCR product was resolved in 1% Agarose gel electrophoresis [30]. The conditions applied for amplification of genomic DNA for inflammatory marker gene expression was given in the (Table 2).…”