Behavioral, Electrophysiological, and Toxicological Responses of Plutella xylostella to Extracts from Angelica pubescens

Abstract

Plutella xylostella L. is a destructive pest affecting cruciferous vegetables, causing massive economic losses worldwide. Plant−based insecticides are considered promising insect control agents. The Angelica pubescens extract inhibited female oviposition, with an oviposition deterrence index (ODI) of 61.65% at 12.5 mg/mL. We aimed to identify the bioactive compounds in A. pubescens extract. The compounds from A. pubescens extract were analyzed using LC−MS techniques. The toxicity and behavioral responses of larvae and adults of P. xylostella to ten compounds were investigated. We found that the caryophyllene oxide and 3,4-dimethoxycinnamic acid inhibited female oviposition; the ODIs were 98.31% and 97.59% at 1.25 mg/mL, respectively. The A. pubescens extract, caryophyllene oxide, and 3,4-dimethoxycinnamic acid caused larval mortality, with LC50 values of 21.31, 4.56, and 5.52 mg/mL, respectively. The EAG response of females was higher than that of males under A. pubescens extract conditions, while the EAG response of males was higher than that of females in caryophyllene oxide and 3,4-dimethoxycinnamic acid conditions. The A. pubescens extract and caryophyllene oxide showed repellent activity against both female and male adults, while the 3,4-dimethoxycinnamic acid did not elicit any notable behavioral responses from P. xylostella adults. A. pubescens extract and caryophyllene oxide are potential insecticides, oviposition deterrents, and behavioral regulators against P. xylostella, and they could be potential candidates for the development of biological insecticides to control P. xylostella.

Study on the trapping effects of Brassica allelochemicals on Plutella xylostella adults

“…It appears that there is an ideal range beyond which extremely high plant volatile chemical levels overwhelm receptors and may cause avoidance (Yang et al, 2004). While there are slight changes in attraction between married and virgin females, which are most likely related to physiological variances, both remain significantly stimulated by essential plant volatile chemicals (Zheng et al, 2023). This demonstrates the DBM olfactory systems' fine-tuned sensitivity to these key plant chemicals.…”

Solubility Behavior, Solvent Effect, and Data Correlation of 3,4-Dimethoxycinnamic Acid in 12 Pure Solvents at Multiple Temperatures

“…6,7 What is more, Zheng et al demonstrated DMCA against adult and larval stages of Plutella xylostella. 8 In 2024, a study showed that DMCA had antiplatelet effects for both ADP and collagen and showed anticoagulant activities. 9 The practical application of chemical substances is always closely related to the interaction between the molecules in the system, and molecular interactions can determine its physical properties such as dissolution, crystal nucleation, and growth.…”

“…In 2020, Medvedeva et al reported that DMCA is natural metabolites present in human blood, which can be the most promising therapeutical for synucleinopathies’ treatment . In addition, ferulic acids and various methylated cinnamic acid derivatives, like DMCA, improve cell viability and indirectly increase the expression of various endogenous antioxidant enzymes. , What is more, Zheng et al demonstrated DMCA against adult and larval stages of Plutella xylostella . In 2024, a study showed that DMCA had antiplatelet effects for both ADP and collagen and showed anticoagulant activities …”