Titanium alloys are extensively employed in machining to produce high-value components, where surface roughness is crucial for their functionality. In this study, the roughness of Ti6Al4V and Ti6Al7Nb turned surfaces was analysed. The goal was to compare the alloy’s roughness behaviour when machined under the same conditions. Therefore, hypothesis testing was performed to assess the equality of the expected values and population median. It was found that, for some parameters, the mean ( RzI, Rt) and median ( Rq, RzD, Pt) roughness was statistically different when changing alloys. However, for others, it did not present a significant difference, in terms of mean ( RmD, Rp) and median ( Ra, Rpm). Consequently, a relevant insight from this study was that the phenomenon interpretation was affected by roughness parameter selection. On the other hand, as expected, the feed rate played a critical role in roughness control. It was observed that Ti6Al7Nb had a smoother topography ( Ra was 0.15 ± 0.01 μm and 0.62 ± 0.04 μm) than Ti6Al4V (0.31 ± 0.02 μm and 0.67 ± 0.03 μm) at 0.05 and 0.15 mm/rev. Yet, at 0.1 mm/rev, this behaviour changed, as Ti6Al4V had a smoother surface ( Ra was 0.40 ± 0.02 μm) than Ti6Al7Nb ( Ra was 0.51 ± 0.03 μm). Finally, the multi-optimization indicated that a low feed rate (0.05 mm/rev) and medium (60 m/min) and high (90 m/min) cutting speed led to lower roughness and higher material removal rate for turning Ti6Al7Nb and Ti6Al7V, respectively. The insights from this study may have a practical application in the manufacturing and quality control of biomedical components.