Behaviour of Aspergillus flavus in presence of Aspergillus niger during biosynthesis of aflatoxin B1

Shantha, T.; Rati, E. R.; Shankar, T.

doi:10.1007/bf00422728

Cited by 22 publications

(6 citation statements)

References 13 publications

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“… Horn and Wicklow (1983) indicated that A. niger lowered substrate to acidic pH value to suppress AFB 1 formation. Shantha et al (1990) demonstrated that oxalic acid and gluconic acid secreted by A. niger could prevent AFB 1 production ( Shantha and Rati, 1990 ; Shantha et al, 1990 ). However, some recent researches indicated that an antifungal peptide (AFP) isolated from A. niger could inhibit the growth of A. flavus and AFB 1 biosynthesis ( Lee et al, 1999 ; Xu et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Variation in fungal microbiome (mycobiome) and aflatoxin in stored in-shell peanuts at four different areas of China

et al. 2015

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The contamination of peanuts with Aspergillus sp. and subsequently aflatoxins is considered to be one of the most serious safety problems in the world. Mycobiome in peanuts is critical for aflatoxin production and food safety. To evaluate the biodiversity and ecological characteristics of whole communities in stored peanuts, the barcoded Illumina paired-end sequencing of the internal transcribed spacer 2 (ITS2) region of rDNA was used to characterize the peanut mycobiome monthly over a period of 1 year at four main peanut grown areas, i.e., Liaoning (LN, North East), Shandong (SD, East), Hubei (HB, Central), and Guangdong (GD, South) provinces. The fungal diversity of peanuts stored in SD was the highest with 98 OTUs and 43 genera, followed by LN, HB and GD. In peanuts stored in SD, Rhizopus, Emericella, and Clonostachys were predominant. In peanuts from LN, Penicillium, Eurotium, and Clonostachys were abundant. In peanuts from HB, Penicillium, Eurotium, and Aspergillus were higher. In GD peanuts, Eurotium, Aspergillus, and Emericella were mainly seen. The abundances of Aspergillus in LN, SD, HB, and GD were 0.53, 6.29, 10.86, and 25.75%, respectively. From the North of China to the South, that increased over the latitude, suggesting that the higher temperature and relative humidity might increase the risk of peanuts contaminated with Aspergillus and aflatoxins. During the storage, Aspergillus levels were higher at 7–12 months than in 0–6 months, suggesting that the risk increases over storage time. At 7–10 months, AFB1 was higher in four areas, while declined further. The reduction of AFB1 might be attributed to the inhibition and degradation of AFB1 by Aspergillus niger or to the combination with the compounds of peanuts. This is the first study that identified the mycobiome and its variation in stored peanuts using ITS2 sequencing technology, and provides the basis for a detailed characterization of whole mycobiome in peanuts.

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Section: Discussionmentioning

confidence: 99%

Variation in fungal microbiome (mycobiome) and aflatoxin in stored in-shell peanuts at four different areas of China

et al. 2015

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“…This finding is similar to various investigators who reported that a number of micro-organisms including bacteria, yeast and moulds, affected the production of aflatoxin in a competitive environment. Aspergillus niger when grown as mixed culture with toxigenic A. flavus inhibits biosynthesis of aflatoxin, owing primarily to its ability to produce inhibitors of aflatoxin biosynthesis and to degrade aflatoxin (Shantha et al 1990). According to Horn and Wicklow (1983), A. niger lowered substrate pH sufficiently and suppressed aflatoxin production.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of aflatoxin B₁production ofAspergillus flavus, isolated from soybean seeds by certain natural plant products

Krishnamurthy

Shashikala

2006

Letters in Applied Microbiology

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Aims: The inhibitory effect of cowdung fumes, Captan, leaf powder of Withania somnifera, Hyptis suaveolens, Eucalyptus citriodora, peel powder of Citrus sinensis, Citrus medica and Punica granatum, neem cake and pongamia cake and spore suspension of Trichoderma harzianum and Aspergillus niger on aflatoxin B1 production by toxigenic strain of Aspergillus flavus isolated from soybean seeds was investigated. Methods and Results: Soybean seed was treated with different natural products and fungicide captan and was inoculated with toxigenic strain of A. flavus and incubated for different periods. The results showed that all the treatments were effective in controlling aflatoxin B1 production. Captan, neem cake, spore suspension of T. harzianum, A. niger and combination of both reduced the level of aflatoxin B1 to a great extent. Leaf powder of W. somnifera, H. suaveolens, peel powder of C. sinensis, C. medica and pongamia cake also controlled the aflatoxin B1 production. Conclusions: All the natural product treatments applied were significantly effective in inhibiting aflatoxin B1 production on soybean seeds by A. flavus. Significance and Impact of the Study: These natural plant products may successfully replace chemical fungicides and provide an alternative method to protect soybean and other agricultural commodities from aflatoxin B1 production by A. flavus.

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“…The three strains were aggressive inhibitors of A. flavus growth, and macroscopic observations of mixed cultures showed highly predominant sporulating of A. niger, whereas AFB 1 was not detected. One strain led to a 90 % inhibition (C mean =1.9 µg mL -1 ) and another in 80 % inhibition (C mean =3.7 µg mL -1 ), primarily due to the action of gluconic acid, which is produced by A. niger (21)(22)(23).…”

Section: Resultsmentioning

confidence: 99%

Interaction Between Certain Moulds and Aflatoxin B1 Producer Aspergillus Flavus NRRL 3251

Cvetnić¹,

Pepeljnjak²

2007

Archives of Industrial Hygiene and Toxicology

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The objective of this study was to evaluate biotic interaction between some mould species and active producer of aflatoxin B 1 Aspergillus flavus NRRL 3251, co-cultured in yeast-extract sucrose (YES) broth. Twenty-five mould strains of Alternaria spp., Cladosporium spp., Mucor spp., A. flavus and A. niger, used as biocompetitive agents, were isolated from outdoor and indoor airborne fungi, scrapings of mouldy household walls, and from stored and post-harvest maize. Aflatoxin B 1 was extracted from mould biomasses with chloroform and detected using the multitoxin TLC method. The results confirm antagonistic interaction between all strains tested. With Alternaria spp. and Cladosporium spp., aflatoxin B 1 production decreased 100 %, compared to detection in a single culture of A. flavus NRRL 3251 (C mean =18.7 µg mL -1 ). In mixed cultures with Mucor spp., aflatoxin B 1 levels dropped to (5.6-9.3) µg mL -1 , and the inhibition was from 50 % to 70 %. Four of five aflatoxin non-producing strains of A. flavus interfered with aflatoxin production in mixed culture, and reduced AFB 1 productivity by 100 %. One strain showed a lower efficacy in inhibiting AFB 1 production (80 %) with a detectable amount of AFB 1 3.7 µg mL -1 when compared to control. A decrease in toxin production was also observed in dual cultivation with A. niger strains. It resulted in 100 % reduction in three strains), 90 % reduction in one strain (C mean =1.9 µg mL -1 ) and 80 % reduction in one strain (C mean =3.7 µg mL -1 ) inhibition.

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Behaviour of Aspergillus flavus in presence of Aspergillus niger during biosynthesis of aflatoxin B1

Cited by 22 publications

References 13 publications

Variation in fungal microbiome (mycobiome) and aflatoxin in stored in-shell peanuts at four different areas of China

Variation in fungal microbiome (mycobiome) and aflatoxin in stored in-shell peanuts at four different areas of China

Inhibition of aflatoxin B₁production ofAspergillus flavus, isolated from soybean seeds by certain natural plant products

Interaction Between Certain Moulds and Aflatoxin B1 Producer Aspergillus Flavus NRRL 3251

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Behaviour of Aspergillus flavus in presence of Aspergillus niger during biosynthesis of aflatoxin B1

Cited by 22 publications

References 13 publications

Variation in fungal microbiome (mycobiome) and aflatoxin in stored in-shell peanuts at four different areas of China

Variation in fungal microbiome (mycobiome) and aflatoxin in stored in-shell peanuts at four different areas of China

Inhibition of aflatoxin B1production ofAspergillus flavus, isolated from soybean seeds by certain natural plant products

Interaction Between Certain Moulds and Aflatoxin B1 Producer Aspergillus Flavus NRRL 3251

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Inhibition of aflatoxin B₁production ofAspergillus flavus, isolated from soybean seeds by certain natural plant products