Behaviour of Guinea Pig T Cells Stimulated by Antigen, Allo-Antigen, and Mitogen

Abstract

The expression of major histocompatibility (MHC) antigens on guinea pig T cells was used as a functional marker for lymphocyte activation. Antigen-stimulated lymphocytes were recovered from guinea pigs responding to the contact sensitizer DNFB, and isolated T cells were then phenotyped using a new anti-guinea pig monoclonal antibody, MSgp7. The level of expression of MHC class II, as defined by the monoclonal antibody, MSgp8, was increased on T cells recovered 4 days after sensitization, as compared with unsensitized controls. The value of this experiment was extended by measuring MHC class II expression on T cells stimulated in vitro by the mitogen concanavalin A, where a clear increase in MSgp8 binding was also observed. Confirmation of the specificity of MSgp8 for guinea pig MHC class II antigens was achieved by studying the inhibitory capacity of this antibody on an MHC class II restricted mixed leucocyte reaction. The combination of antibodies MSgp7 and MSgp8 with flow cytometry could be applied to other guinea pig experimental models to quantitate the expression of MHC class II antigens on T cells to determine their putative value in disease manifestation.

Tools for cellular immunology and vaccine research the in the guinea pig: Monoclonal antibodies to cell surface antigens and cell lines

“…Antibodies directed against proteins encoded in the Major Histocompatibility Complex (MHC) have been shown to be important for the analysis of MHC restricted antigen presentation. As expected, most anti-MHC class I and anti-MHC class II antibodies interfere with the function of class I or class II restricted T lymphocytes [42], [47] and [48], i.e. inhibit the response to antigen presented on MHC molecules.…”

Major histocompatibility complex class II antigen expression during potentiation of line-10 tumor immunity after intralesional administration of bacillus Calmette-Guérin

“…mAb to line-10 antigens 10TL1, 10TL40 and 10TL43 [29,42] were diluted 1 : 500. mAb to guinea pig MHC class II antigens 25E3 and 27E7 were kindly provided by Dr. Shevach [6] (National Institute of Allergy and Infectious Diseases, Bethesda, Md., USA) and diluted 1 : 500. mAb OKIa to human MHC class II antigens were obtained from Ortho Diagnostics Systems (Beerse, Belgium) and diluted l : 80. MSgp4, reacting with MHC class I antigens from guinea pigs was kindly provided by Dr Baker [20] (Hunterian Institute, Royal College of Surgeons of England, London, UK). mAb to T cell (pan T) antigen (CT5) [40] was diluted 1 : 200. mAb to macrophage antigen (305) [27] was diluted 1:200. mAb to IgM B lymphocytes (31D2) was diluted 1:200. mAb 305 and 31D2 were kindly provided by Dr R. Burger (Institute for Immunology, University of Heidelberg, FRG).…”

Major histocompatibility complex class II antigen expression on leucocyte subpopulations in the draining lymph node and tumour in the early phase of bacillus-Calmette-Guérin-induced tumour regression

“…MSgp9 and 31D2 are mAbs reacting with all B cells and with IgM B cells, respectively [10,25]. M305 is a mAb detecting guinea-pig macrophages [24], and MSgp4 and 25E3 are mAbs detecting guinea-pig MHC class I and II antigens, respectively [8,18]. 10TL40 and 10TIA3 are mAbs reacting with line 10 antigens, while 10TL1 also binds to line 10 and L2C cells [28].…”