Behaviour of phenylalanyl‐tRMA from <i>Halobacterium cutirubrum</i> on BD‐cellulose

Uncharged rat liver tRNA can be eluted from BDcellulose columns with 0.8 M sodium chloride, with the exception of tRNAPhe, which can only be eluted with 0.8 M sodium chloride containing ethanol. When the uncharged rat liver tRNA is reacted with the N-hydroxysuccinimide ester of phenoxyacetic acid, there is an increase in the amount of tRNA that is eluted in the presence of ethanol. Greater than 5 0 z of the tRNAs for tyrosine, threonine, isoleucine, cysteine, and asparagine can now be eluted only in the presence of ethanol. Only a small fraction of the tRNA from Escherichin coli and yeast shifted into the ethanol fraction after reac-T here have been several reports of the presence of certain bases in transfer ribonucleic acid that can undergo acylation reactions. However, the nature of these bases has not been fully characterized, nor has it been determined whether these are rare bases, or one or more of the four major bases, that are especially reactive because of their conformation in tRNA. With the advent of BD-cellulose' columns, which have affinity

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Heterologous mischarging as a means of tRNA fractionation. I. Behaviour of E.coli phenylalanyl-tRNA₁^Valon BD-cellulose

Rosenmann

Krauskopf²

1974

Nucl Acids Res

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The chromatographic behaviour of E.coli tRNA(Val) (1), Val-tRNA(Val) (1) and Phe-tRNA(Val) (1) was studied on BD-cellulose columns. At pH 4.0 and 4 degrees C the elution position of Phe-tRNA(Val) (1) was not affected by the presence of absence of Mg(2+), whereas Val-tRNA(Val) (1) was slightly retarded when Mg(2+) was ommited. It is postulated that the amino acid and its nature influence the structure that the aminoacyl-tRNA assumes. Under suitable conditions the heteroaminoacylated Phe-tRNA(Val) (1) eluted significantly later than other tRNAs. This fact showed that heterologous mischarging can be a useful step in tRNA purification methods.

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Behaviour of phenylalanyl‐tRMA from Halobacterium cutirubrum on BD‐cellulose

Cited by 4 publications

References 10 publications

Physiology of Halobacteriaceae

Physiology of Halobacteriaceae

Acylation of transfer ribonucleic acid with the N-hydroxysuccinimide ester of phenoxyacetic acid

Heterologous mischarging as a means of tRNA fractionation. I. Behaviour of E.coli phenylalanyl-tRNA₁^Valon BD-cellulose

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Behaviour of phenylalanyl‐tRMA from Halobacterium cutirubrum on BD‐cellulose

Cited by 4 publications

References 10 publications

Physiology of Halobacteriaceae

Physiology of Halobacteriaceae

Acylation of transfer ribonucleic acid with the N-hydroxysuccinimide ester of phenoxyacetic acid

Heterologous mischarging as a means of tRNA fractionation. I. Behaviour of E.coli phenylalanyl-tRNA1Valon BD-cellulose

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Heterologous mischarging as a means of tRNA fractionation. I. Behaviour of E.coli phenylalanyl-tRNA₁^Valon BD-cellulose