Behaviour of the new asbestos amphibole fluoro-edenite in different lung cell systems

Cardile, Venera; Renis, Marcella; Scifo, Christian; Lombardo, Laura; Gulino, Rosario; Mancari, Barbara; Panico, Annamaria

doi:10.1016/j.biocel.2003.09.007

Cited by 48 publications

(45 citation statements)

References 34 publications

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“…H and 100.6 MHz for 13 C. Chemical shifts are reported in ppm and coupling constants (J) are given in Hz. HRMS were recorder on a MAT 95XP, Thermo Finnigan spectrometer and represented at m/z (% rel.…”

Section: Supplementary Informationmentioning

confidence: 99%

“…max values are expressed in cm -1 . 1 H and 13 C NMR spectra were recorded in CDCl 3 solutions and referenced to the residual peak of CHCl 3 at 7.26 ppm and 77.00 ppm for 1 H and 13 C, respectively, on a Bruker Avance 400 Digital NMR spectrometer, operating at 400. …”

Section: Generalmentioning

confidence: 99%

“…13 Briefly, the cells were set up 6 x 10 3 cells per well of a 96-well, flat-bottomed 200 µL microplate. Cells were incubated at 37°C in a humidified 5% CO 2 /95% air mixture and treated with doxorubicin, used as a positive control, and the compounds (1-6) at different concentrations for 72 hours.…”

Section: Mtt Bioassaymentioning

confidence: 99%

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Eugenol and its synthetic analogues inhibit cell growth of human cancer cells (Part I)

Cardile

Gallardo

Cardona

et al. 2008

J. Braz. Chem. Soc.

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Propriedades antioxidante e anticancerígena foram relatadas para o Eugenol (4-alil-2-metoxifenol) (1). Na tentativa de aumentar a atividade intrínseca deste composto natural, alguns derivados foram sintetizados. O eugenol foi extraído do óleo de cravo da Índia e seus análogos (2-6) foram obtidos por reações de acetilação e nitração. Eugenol (1) e seus análogos (2-6) foram avaliados in vitro frente a duas linhagens de células de câncer humanas: DU-145 (células de câncer de próstata, insensíveis a andrógeno) e KB (células de carcinoma escamoso oral). A viabilidade celular foi avaliada por ensaios com sal de tetrazólio. A liberação de desidrogenase lática (LDH) também foi investigada para avaliar a toxicidade celular como um resultado do rompimento celular subseqüente à ruptura da membrana. Todos os compostos apresentaram atividade inibitória sobre o crescimento das células cancerosas examinadas. Os resultados obtidos demonstram que os compostos 5-alil-3-nitrobenzeno-1,2-diol (3) e acetato de 4-alil-2-metoxi-5-nitrofenol (5) foram significativamente mais ativos que o eugenol (p < 0,001), com valores de IC 50 em células DU-145 de 19,02 × 10 -6 e 21,5 × 10 -6 mol L -1 respectivamente, sugerindo que a presença dos grupos nitro e hidroxila podem ser importantes na atividade destes compostos. Os resultados também parecem indicar que a morte por apoptose está sendo induzida em células KB e DU-145. Nas condições experimentais avaliadas, não foi observado qualquer aumento estatisticamente significativo na liberação de LDH pelas células de câncer quando tratadas com eugenol e seus análogos.Eugenol (4-allyl-2-methoxyphenol) (1) has been reported to possess antioxidant and anticancer properties. In an attempt to enhance intrinsic activity of this natural compound, some derivatives were synthesized. Eugenol was extracted from cloves oil and further, the eugenol analogues (2-6) were obtained through acetylation and nitration reactions. Eugenol (1) and its analogues (2-6) were examined by in vitro model of cancer using two human cancer cell lines: DU-145 (androgeninsensitive prostate cancer cells) and KB (oral squamous carcinoma cells). Cell viability, by tetrazolium salts assay, was measured. Lactic dehydrogenase (LDH) release was also investigated to evaluate the presence of cell toxicity as a result of cell disruption, subsequent to membrane rupture. In the examined cancer cells, all compounds showed cell-growth inhibition activity. The obtained results demonstrate that the compounds 5-allyl-3-nitrobenzene-1,2-diol (3) and 4-allyl-2-methoxy-5-nitrophenyl acetate (5) were significantly (p < 0,001) more active than eugenol, with IC 50 values in DU-145 cells of 19.02 x 10 -6 and 21.5 × 10 -6 mol L -1 , respectively, and in KB cells of 18.11 × 10 -6 and 21.26 × 10 -6 mol L -1 , respectively, suggesting that the presence of nitro and hydroxyl groups could be important in the activity of these compounds. In addition, our results seem to indicate that apoptotic cell demise appears to be induced in KB and DU-145 cells. In fact, in ou...

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Section: Supplementary Informationmentioning

confidence: 99%

Section: Generalmentioning

confidence: 99%

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Eugenol and its synthetic analogues inhibit cell growth of human cancer cells (Part I)

Cardile

Gallardo

Cardona

et al. 2008

J. Braz. Chem. Soc.

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“…Furthermore, there are other occurrences connected with volcanic deposits that are limited in extension, but become important for their potential danger are, such as at Biancavilla (Catania, Sicily). Here, irrespectively of the relatively limited extension, the pathological effects on the population has been well documented (Comba et al, 2003, Cardile et al, 2004. decomposed using Gaussian components…”

Section: Discussionmentioning

confidence: 99%

Asbestiform tremolite within the Holocene late pyroclastic deposits of Colli Albani volcano (Latium, Italy): occurrence and crystal chemistry

Ventura

Caprilli

Bellatreccia

et al. 2014

Rend. Fis. Acc. Lincei

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This work relates the occurrence and the characterization of fibrous tremolite within the latest pyroclastic deposits of the Colli Albani (Alban Hills) volcano, to the south-east of Rome (Italy). These mineralizations were observed during a systematic rock-sampling undertaken to complete the geological survey for the new 1:50 000 map of this volcanic area. The examined specimens were collected inside distal deposits correlated to the last Albano Maar activity, which are geographically located within the boundaries of the Nemi community. Tremolite occurs within both carbonate ejecta and G. Della Ventura (☺) Dipartimento di Scienze, Università di Roma Tre, Largo S. Leonardo Murialdo 1, I-00146 ROMA e-mail: giancarlo.dellaventura@uniroma3.it 2 the host pyroclastic rocks. It shows up as whitish to light gray coloured aggregates of crystals with fibrous aspect and sericeous brightness. Due to the extremely small crystal dimensions, never exceeding 0.5 µm in diameter, the micro-chemical composition of the fibres could be obtained only by combining P-XRD, SEM-EDX and FTIR methods.Infrared spectroscopy, in particular, proved to be a valuable technique to characterize the studied amphibole. The composition determined is that of a Fe-free F-rich (c. 53%) tremolite with significant (c. 20%) richterite components in solid-solution. The occurrence of fibrous tremolite in an inhabited place, occurring as natural geological material rather than being due to anthropogenic pollution, should be examined with concern, because it implies complex health and legal responsibilities in the case of mobilization due to extreme climatic events.

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“…These effects are related to the ability of fibres to disrupt mitotic mechanisms, stimulate host cell proliferation, induce release of free radicals (resulting in DNA damage), and prolong the release of cytokines and growth factors (45,46). In vivo and in vitro analysis of the biological reactivity of fluoro-edenite fibres has also demonstrated that they are able to cause the production of reactive oxygen species, stimulation of the intrinsic and extrinsic apoptosis pathways, and induction of inflammatory processes through the activation of specific cytokines (47)(48)(49)(50)(51).…”

Section: Risk Indicators --------------------------------------------mentioning

confidence: 99%

Detection of pleural plaques in workers exposed to inhalation of natural fluoro-edenite fibres

et al. 2015

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Abstract. Fluoro-edenite is a natural mineral species initially isolated in Biancavilla, Sicily. The fibres are similar in size and morphology to certain amphibolic asbestos fibres, the inhalation of which may cause chronic inflammation and cancer. Occupational asbestos exposure is known to be associated with pleural and lung diseases, including pleural plaques. The aim of this study was to report the pleural and lung parenchymal lesions detected by high-resolution computed tomography (HRCT) in a group of construction workers exposed to fluoro-edenite. Information regarding life habits and occupational history was collected from 43 workers enrolled into the study. The participants underwent physical examination, blood analysis, search for uncoated fibres and ferruginous bodies in the sputum, pulmonary function tests, including diffusion capacity for carbon monoxide (TL CO ), and HRCT chest imaging. A general descriptive outcome analysis was also conducted; a prevalence ratio (PR) with 95% confidence interval and a two-tailed test P-value were calculated for pleural plaques using log-binomial regression, measuring plaque size and thickness, and cumulative exposure index (CEI). The mean values of the functional respiratory tests were within the normal range for all participants. A restrictive ventilatory defect was identified in two (5%) subjects and an obstructive ventilatory defect in three (7%) subjects. TL CO was reduced in two additional participants. Fibres were detected in 19 (44%) of subjects. Pleural involvement was documented in 39 (91%) workers, of whom 31 (72%) had bilateral plaques. Calcifications were detected in 25 (58%) of these participants. PR indicated a progressive increase in the risk of developing pleural lesions with rising CEI, i.e. length of exposure. The present findings demonstrate for the first time the presence of pleural plaques in the lungs of subjects exposed to fluoro-edenite fibres, and not to asbestos, through residing in Biancavilla and through their occupation.

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Behaviour of the new asbestos amphibole fluoro-edenite in different lung cell systems

Cited by 48 publications

References 34 publications

Eugenol and its synthetic analogues inhibit cell growth of human cancer cells (Part I)

Eugenol and its synthetic analogues inhibit cell growth of human cancer cells (Part I)

Asbestiform tremolite within the Holocene late pyroclastic deposits of Colli Albani volcano (Latium, Italy): occurrence and crystal chemistry

Detection of pleural plaques in workers exposed to inhalation of natural fluoro-edenite fibres

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