Behaviour of the oxidant scavenger metallothionein in hypoxia-induced neotropical fish

Hauser‐Davis, Rachel Ann; Bastos, Frederico F.; Dantas, Rafael Ferreira; Leitão, Santiago Alonso Tobar; Neto, Jayme da Cunha Bastos; Bastos, Vera Lúcia Freire da Cunha; Ziolli, Roberta Lourênço; Arruda, Marco Aurélio Zezzi

doi:10.1016/j.ecoenv.2014.01.015

Cited by 17 publications

(3 citation statements)

References 34 publications

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“…Secondly, upregulation of the CdMT gene may have happened shortly after snails were exposed to oxygen-free conditions or after the termination of the experiment. Results also may indicate a decrease of CdMT gene expression after 12 hours (see Fig 6B ) which was reported for a neotropical fish species and was explained by cellular defense signaling pathways due to hypoxic stress [ 75 ].…”

Section: Discussionsupporting

confidence: 57%

Physiological, Diurnal and Stress-Related Variability of Cadmium-Metallothionein Gene Expression in Land Snails

et al. 2016

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The terrestrial Roman snail Helix pomatia has successfully adapted to strongly fluctuating conditions in its natural soil habitat. Part of the snail’s stress defense strategy is its ability to express Metallothioneins (MTs). These are multifunctional, cysteine-rich proteins that bind and inactivate transition metal ions (Cd2+, Zn2+, Cu+) with high affinity. In Helix pomatia a Cadmium (Cd)-selective, inducible Metallothionein Isoform (CdMT) is mainly involved in detoxification of this harmful metal. In addition, the snail CdMT has been shown to also respond to certain physiological stressors. The aim of the present study was to investigate the physiological and diurnal variability of CdMT gene expression in snails exposed to Cd and non-metallic stressors such as desiccation and oxygen depletion. CdMT gene expression was upregulated by Cd exposure and desiccation, whereas no significant impact on the expression of CdMT was measured due to oxygen depletion. Overall, Cd was clearly more effective as an inducer of the CdMT gene expression compared to the applied non-metallic stressors. In unexposed snails, diurnal rhythmicity of CdMT gene expression was observed with higher mRNA concentrations at night compared to daytime. This rhythmicity was severely disrupted in Cd-exposed snails which exhibited highest CdMT gene transcription rates in the morning. Apart from diurnal rhythmicity, feeding activity also had a strong impact on CdMT gene expression. Although underlying mechanisms are not completely understood, it is clear that factors increasing MT expression variability have to be considered when using MT mRNA quantification as a biomarker for environmental stressors.

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Section: Discussionsupporting

confidence: 57%

Physiological, Diurnal and Stress-Related Variability of Cadmium-Metallothionein Gene Expression in Land Snails

et al. 2016

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“…This enzyme was associated with mud in all PCAs and caused lipoperoxidation in hepatopancreas in station PM. Hypoxic environment tend to diminish GSH levels, indicating that LPO in this case was not caused by low oxygen levels (Hauser-Davis et al, 2014). Although, this enzyme has also the capacity to chelate metals (Sterling et al, 2010;Wang et al, 2008), inhibiting the detoxification activation system.…”

Section: Condition Factormentioning

confidence: 73%

Análises Geoquímicas e respostas Ecotoxicológicas na Avaliação da Qualidade Ambiental de uma Área Estuarina Protegida

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“…As alterações nos níveis de proteínas de alguns órgãos, como fígado e rim, são capazes de indicar a indução ou inibição de certas proteínas e/ou enzimas, de forma que níveis elevados podem sugerir a síntese de enzimas de biotransformação, que podem auxiliar no processo de destoxificação do organismo, tornando-se, portanto, importantes na avaliação de efeitos subletais de contaminantes ambientais em organismos aquáticos (Hauser-Davis, 2013;Hauser-Davis et al, 2014).…”

Section: Estresse Oxidativo Em Organismos Expostos à Metaisunclassified

Estudo Da Distribuição Subcelular E Destoxificação Bioquímica Elementar Em Duas Espécies De Golfinho Do Sudeste Brasileiro

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Behaviour of the oxidant scavenger metallothionein in hypoxia-induced neotropical fish

Cited by 17 publications

References 34 publications

Physiological, Diurnal and Stress-Related Variability of Cadmium-Metallothionein Gene Expression in Land Snails

Physiological, Diurnal and Stress-Related Variability of Cadmium-Metallothionein Gene Expression in Land Snails

Análises Geoquímicas e respostas Ecotoxicológicas na Avaliação da Qualidade Ambiental de uma Área Estuarina Protegida

Estudo Da Distribuição Subcelular E Destoxificação Bioquímica Elementar Em Duas Espécies De Golfinho Do Sudeste Brasileiro

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