Behavioural assessment of dimethylsulfoxide neurotoxicity in rats

Authier, Nicolas; Dupuis, Erwan; Kwasiborski, A.; Eschalier, Alain; François, Clément

doi:10.1016/s0378-4274(02)00052-8

Cited by 31 publications

(17 citation statements)

References 16 publications

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“…Behavioural effects of DMSO have been reported in adult rats, including actions that might reflect altered cortical and/or hippocampal activity [17], [18]. Importantly significant DMSO exposure is a consequence of stem cell therapies [19] including bone marrow transplantation such as that provided to children with neuroblastoma or Ewing's sarcoma.…”

Section: Discussionmentioning

confidence: 99%

Low Concentrations of the Solvent Dimethyl Sulphoxide Alter Intrinsic Excitability Properties of Cortical and Hippocampal Pyramidal Cells

et al. 2014

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Dimethylsulfoxide (DMSO) is a widely used solvent in biology. It has many applications perhaps the most common of which is in aiding the preparation of drug solutions from hydrophobic chemical entities. Recent studies have suggested that this molecule may be able to induce apoptosis in neural tissues urging caution regarding its introduction into humans, for example as part of stem cell transplants. Here we have used in vitro electrophysiological methods applied to murine brain slices to examine whether a few hours treatment with 0.05% DMSO (a concentration regarded by many as innocuous) alters intrinsic excitability properties of neurones. We investigated pyramidal neurones in two distinct brain regions, namely area CA1 of the hippocampus and layer 2 of perirhinal cortex. In the former there was no effect on resting potential but input resistance was decreased by DMSO pre-treatment. In line with this action potential count for any level of depolarizing current stimulus was reduced by ∼25% following DMSO treatment. Ih-mediated “sag” was also increased in CA1 pyramids and action potential waveform analysis demonstrated that DMSO treatment moved action potential threshold towards resting potential. In perirhinal cortex a decreased action potential output for various depolarizing current stimuli was also seen. In these cells action potential threshold was unaltered by DMSO but a significant increase in action potential width was apparent. These data indicate that pre-treatment with this widely employed solvent can elicit multifaceted neurophysiological changes in mammalian neurones at concentrations below those frequently encountered in the published literature.

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Section: Discussionmentioning

confidence: 99%

Low Concentrations of the Solvent Dimethyl Sulphoxide Alter Intrinsic Excitability Properties of Cortical and Hippocampal Pyramidal Cells

et al. 2014

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“…Second, doses of DSMO used for medical treatments (e.g., 2-6 g/kg/20 days; Brien et al 2008), which have sometimes been associated with adverse side effects, are over five orders of magnitude greater than those used here. Third, high doses of DMSO administered to adult rats for long durations have produced little or no evidence of neurotoxic effects (Authier et al 2002).…”

Section: Discussionmentioning

confidence: 99%

Anxiolytic-like effects of somatostatin isoforms SST 14 and SST 28 in two animal models (Rattus norvegicus) after intra-amygdalar and intra-septal microinfusions

2011

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SST receptors in the septum and amygdala are responsive to both SST 14 and SST 28, but not those in the striatum. Although no obvious differences in the anxiolytic-like effects of the isoforms were detected, quantitative or even qualitative differences in their specific anxiolytic effects may occur in different sub-regions of the septum and amygdala, as has been found for benzodiazepine anxiolytics.

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“…Extremely high doses of DMSO (1mg/g) were used to study the effects of DMSO in inducing acute-phase response, a non-specific reaction of an organism to stress, in ten weeks old male Wistar rats 23 . In the present study the DMSO was dissolved in saline, resulting in a solution of 3%, quite below the maximum concentration used by Authier et al 22 Therefore, the maximum dose of DMSO administered to a 200g rat was 6 mg.…”

Section: Discussionmentioning

confidence: 72%

“…Park et al 21 studied the mechanism of DMSO in the prevention of acetaminophen-induced hepatotoxicity in mice treated with DMSO 1ml/Kg and conclude that it could be related to the ability to competitively inhibit acetaminopheninduced hepatotoxicity (APAP) bioactivation or to directly scavenge free radicals produced during APAP metabolism. Authier et al 22 assessed DMSO neurotoxicity in rats, using increasing doses of DMSO (1.8%, 3.6% and 7.2% solutions) for each group of 7 rats. DMSO was given daily for 10 days.…”

Section: Discussionmentioning

confidence: 99%

Dimethylsulfoxide attenuates ischemia-reperfusion injury in rat testis

Guimarães¹,

Kimura²,

Vasconcelos³

2010

Acta Cir. Bras.

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Purpose:To evaluate the protective role of dimethylsulfoxide (DMSO) in a rat model of testis ischemia/reperfusion (I/R). Methods: Twenty-four male Wistar rats were randomized in two equal groups. Control rats (G-1) received saline 2.0 ml intraperitoneally (ip) 21, 9 and 1 h before torsion. Experimental rats (G-2) received ip injections of 3% aqueous solution of DMSO, 0.1ml/10g body weight. Saline was added to complete 2.0ml when necessary. I/R injury was induced in anesthetized rats by torsion of the right testis lasting 3 hours. Testis and blood samples were collected at the end of ischemia (T-0) and 3 hours later (T-3) for assessment of testis malonaldehyde (MDA), reduced glutathione (GSH), and plasma total antioxidant power (TAP). Results: MDA levels decreased significantly in G-2 rats compared with G-1 animals in all time-points. GSH levels increased significantly in T-0 and T-3 time-points in DMSO pretreated rats compared with G-1 rats. GSH levels increased significantly during reperfusion in G-2 rats. TAP was similar in both groups denoting absence of systemic effects in this study. Conclusion: Pretreatment with DMSO reduces testis lipid peroxidation and oxidative stress caused by torsion/detorsion of the testis. Key words: Antioxidants. Testis. Oxidative Stress. Malondialdehyde. Glutathione. Rats. RESUMO Objetivo:Avaliar o papel protetor do dimetilsulfóxido (DMSO) utilizando um modelo de isquemia/reperfusão (I/R) do testículo do rato. Métodos: Vinte e quatro ratos machos Wistar, randomizados em dois grupos iguais e tratados com solução salina 2,0 ml (grupo Controle-G-1) ou solução de DMSO (3%, 0,1ml/10g peso corporal), acrescida de solução salina até completar 2,0 ml (grupo Experimento-G-2) por via intraperitoneal, as 21, 9 e 1 h antes da torção. .A lesão de I/R foi induzida por torção do testículo direito e mantida por 3 horas, em ratos previamente anestesiados. Amostras (sangue e testículo direito) foram coletadas ao término da isquemia (T-0) e após 3 horas de reperfusão (T-3) para determinação das concentrações de malonaldeído (MDA), glutationa reduzida (GSH) e capacidade antioxidante total do plasma (CAT). Resultados: As concentrações de MDA diminuíram significativamente nos ratos do G-2. comparados ao G-1, nos tempos estudados. As concentrações de GSH aumentaram significativamente nos testículos dos ratos do G-2 comparado aos controles no T-0 e no T-3 e durante a reperfusão no G-2. Os valores obtidos na análise do CAT foram semelhantes. Conclusão: O pré-tratamento com DMSO reduz a peroxidação lipídica e o estresse oxidativo decorrentes da torção/destorção do testículo, no rato.

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Behavioural assessment of dimethylsulfoxide neurotoxicity in rats

Cited by 31 publications

References 16 publications

Low Concentrations of the Solvent Dimethyl Sulphoxide Alter Intrinsic Excitability Properties of Cortical and Hippocampal Pyramidal Cells

Low Concentrations of the Solvent Dimethyl Sulphoxide Alter Intrinsic Excitability Properties of Cortical and Hippocampal Pyramidal Cells

Anxiolytic-like effects of somatostatin isoforms SST 14 and SST 28 in two animal models (Rattus norvegicus) after intra-amygdalar and intra-septal microinfusions

Dimethylsulfoxide attenuates ischemia-reperfusion injury in rat testis

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