Behavioural Pharmacology in Classical Conditioning of the Proboscis Extension Response in Honeybees (&lt;em&gt;Apis mellifera&lt;/em&gt;)

Felsenberg, Johannes; Gehring, Katrin B.; Antemann, Victoria; Eisenhardt, Dorothea

doi:10.3791/2282-v

Cited by 3 publications

(1 citation statement)

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“…Proboscis extension response (PER), which is a conditioned response to conditional and unconditional stimuli, is typically used to investigate the olfactory learning behavior of honey bees under controlled laboratory conditions. Honey bees establish a link between odor and reward after several cycles of training [8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Differential Brain Expression Patterns of microRNAs Related to Olfactory Performance in Honey Bees (Apis mellifera)

Huang

Wang

Qiu

et al. 2023

Genes

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MicroRNAs (miRNAs) play a vital role in the nerve regulation of honey bees (Apis mellifera). This study aims to investigate the differences in expression of miRNAs in a honey bee’s brain for olfactory learning tasks and to explore their potential role in a honey bee’s olfactory learning and memory. In this study, 12 day old honey bees with strong and weak olfactory performances were utilized to investigate the influence of miRNAs on olfactory learning behavior. The honey bee brains were dissected, and a small RNA-seq technique was used for high-throughput sequencing. The data analysis of the miRNA sequences revealed that 14 differentially expressed miRNAs (DEmiRNAs) between the two groups, strong (S) and weak (W), for olfactory performance in honey bees were identified, which included seven up-regulated and seven down-regulated. The qPCR verification results of the 14 miRNAs showed that four miRNAs (miR-184-3p, miR-276-3p, miR-87-3p, and miR-124-3p) were significantly associated with olfactory learning and memory. The target genes of these DEmiRNAs were subjected to the GO database annotation and KEGG pathway enrichment analyses. The functional annotation and pathway analysis showed that the neuroactive ligand-receptor interaction pathway, oxidative phosphorylation, biosynthesis of amino acids, pentose phosphate pathway, carbon metabolism, and terpenoid backbone biosynthesis may be a great important pathway related to olfactory learning and memory in honey bees. Our findings together further explained the relationship between olfactory performance and the brain function of honey bees at the molecular level and provides a basis for further study on miRNAs related to olfactory learning and memory in honey bees.

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Section: Introductionmentioning

confidence: 99%

Differential Brain Expression Patterns of microRNAs Related to Olfactory Performance in Honey Bees (Apis mellifera)

Huang

Wang

Qiu

et al. 2023

Genes

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Mapping the effects of ozone pollution and mixing on floral odour plumes and their impact on plant-pollinator interactions

Langford

Ryalls

Mullinger

et al. 2023

Environmental Pollution

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The Combined Effects of Varroa destructor Parasitism and Exposure to Neonicotinoids Affects Honey Bee (Apis mellifera L.) Memory and Gene Expression

Morfin

Guzmán-Novoa

2020

Biology

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Honey bees (Apis mellifera L.) are exposed biotic and abiotic stressors but little is known about their combined effect and impact on neural processes such as learning and memory, which could affect behaviours that are important for individual and colony survival. This study measured memory with the proboscis extension response (PER) assay as well as the expression of neural genes in bees chronically exposed to three different sublethal doses of the insecticide clothianidin and/or the parasitic mite Varroa destructor. The proportion of bees that positively responded to PER at 24 and 48 h post-training (hpt) was significantly reduced when exposed to clothianidin. V. destructor parasitism reduced the proportion of bees that responded to PER at 48 hpt. Combined effects between the lowest clothianidin dose and V. destructor for the proportion of bees that responded to PER were found at 24 hpt. Clothianidin, V. destructor and their combination differentially affected the expression of the neural-related genes, AmNrx-1 (neurexin), AmNlg-1 (neuroligin), and AmAChE-2 (acetylcholinesterase). Different doses of clothianidin down-regulated or up-regulated the genes, whereas V. destructor tended to have a down-regulatory effect. It appears that clothianidin and V. destructor affected neural processes in honey bees through different mechanisms.

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Behavioural Pharmacology in Classical Conditioning of the Proboscis Extension Response in Honeybees (<em>Apis mellifera</em>)

Cited by 3 publications

References 1 publication

Differential Brain Expression Patterns of microRNAs Related to Olfactory Performance in Honey Bees (Apis mellifera)

Differential Brain Expression Patterns of microRNAs Related to Olfactory Performance in Honey Bees (Apis mellifera)

Mapping the effects of ozone pollution and mixing on floral odour plumes and their impact on plant-pollinator interactions

The Combined Effects of Varroa destructor Parasitism and Exposure to Neonicotinoids Affects Honey Bee (Apis mellifera L.) Memory and Gene Expression

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