Beitrag zur Bestimmung von Schwefelkohlenstoff im Blut

Pharmacokinetics of inhaled carbon disulphide in rats in relation to its inhibitory effect on the side-chain oxidation of hexobarbital

“…Analytical Procedures C 5 2 was removed from the blood and the liver homogenate, respectively, by means of nitrogen gas and then determined quantitatively by the colorimetric method of Massmann & Strenge ( 1957) This involved addition of the following materials to 1 ml of blood or liver homogenate: 1) 2 ml doubly distilled water to achieve haemolysis or to obtain the same volume as for the blood samples; 2) 2 ml ethanol (A R , Merck, Darmstadt) for protein precipitation; 3) 2 drops of octanol (A R , Merck, Darmstadt) for reduction of surface tension; 4) 4 ml 5 N HC 1 to set free the so-called acid-soluble C 52 fraction (B Using & Bohlander, 1952) The time required to expel the free C 5 2 and the acid-soluble C 5 2 fraction, the latter being removed immediately after the free C 5 2 , was 2 hrs in each case Taken together the 2 C 5 2 concentrations yielded the total amount of C 5 2 detectable. 1 4 C 5 2 radioactivity was measured in the exhaled air and in the liver (total homogenate) and its fractions ( 9,000 x g sediment, 78,000 x g supernatant, microsomes) Wet organic samples were dissolved in toluene TM 100 (Packard Instr Co , Inc ) The scintillator fluid used was a 50:31 5:1 (by vol ) toluene-isopropanol-2 N HC 1 mixture containing 0 24 % (w/v) 2,5-diphenyloxazole and 0 006 % (w/v) 1,4-bis-( 4-methyl-5-phenyl-2-oxazolyl)-benzene (Packard) All samples were counted in a Packard Tri-Carb liquid scintillation spectrometer (model 3380) and corrected for quenching by the use of internal standards The labelled exhaled air was passed through the scintillation fluid to which was added 0 5 % (v/v) piperidine (A R , Merck, Darmstadt) and O 001 % (w/v) copper acetate (A.R , Merck, Darmstadt) to allow the formation of a yellow complex (Massmann & Strenge, 1957).…”

“…1 4 C 5 2 radioactivity was measured in the exhaled air and in the liver (total homogenate) and its fractions ( 9,000 x g sediment, 78,000 x g supernatant, microsomes) Wet organic samples were dissolved in toluene TM 100 (Packard Instr Co , Inc ) The scintillator fluid used was a 50:31 5:1 (by vol ) toluene-isopropanol-2 N HC 1 mixture containing 0 24 % (w/v) 2,5-diphenyloxazole and 0 006 % (w/v) 1,4-bis-( 4-methyl-5-phenyl-2-oxazolyl)-benzene (Packard) All samples were counted in a Packard Tri-Carb liquid scintillation spectrometer (model 3380) and corrected for quenching by the use of internal standards The labelled exhaled air was passed through the scintillation fluid to which was added 0 5 % (v/v) piperidine (A R , Merck, Darmstadt) and O 001 % (w/v) copper acetate (A.R , Merck, Darmstadt) to allow the formation of a yellow complex (Massmann & Strenge, 1957).…”