“…The number of layers surrounding the egg of ascarids has been reported as three (Nelson, 1851, Ascaris 'mystax;Leuckart, 1886, Ascaris luwibricoides; Ackert, 1931, Ascaridia lineata;Wottge, 1937, Parascaris megalocephala;Christenson, 1940, Ascaris lumbricoides), four (Kreuzer, 1953, Ascaris lu~.izbricoides; Frenzen, 1954, Ascaridia galli, and five (Zavadovsky, 1928, Ascwis). A variety of techniques has been used by different authors in studying the egg layers, including microscopic observations of the penetration of substances into the egg, polarizing and fluorescent microscopy, centrifugation, and chemical tests on the membranes.…”