The purpose of this study was to identify the influence of karst landscape on the sustainable development of the Bela krajina region, southeast Slovenia. In order to better understand the influence of karst landscape on sustainable development we used three approaches: (1) the Karst Disturbance Index, (2) a quantitative analysis by using sustainable development indicators and (3) a qualitative analysis using structured interviews. The Karst Disturbance Index classified the degree of disturbance in Bela krajina as low. According to sustainable development indicators we found differences in the structure related to economy, population and environment within the region. And, according to the qualitative analysis, the negative impacts of the karst landscape on sustainable development are mainly associated with hampered agriculture and the positive with tourism; thus, karst landscape cannot only be seen as a limiting factor, since it also has development potential. Regional development in karst areas should therefore be adapted to their specificities and take into account their vulnerability.