HIV become iceberg phenomenon. All HIV infected people have risk illness and die. Data of WHO 2018 were 37.9 million PLWH and 23.3 million on antiretroviral treatment. Every month about 8-15 new peoplegotnew HIV infection in Probolinggo district, Indonesia. HIV cummulative in Probolinggo at January 2018 were 1.140 people and only 60% ARV active and 15% lost to follow up. Many factors that influence adherence and many impact of it.. The aim to analysis psychosocial factors to increase adherence antiretroviral treatment on New PLWH infection.This method use observational study. The population were 61 PLWH on ARV treatment in Clini VCT Waluyojati General Hospital at January-May 2018, and sample were 54 people, sistematic random sampling, instrument quessionairres, observational sheet, medical record, independent variable were level of knowledge, age, sex, ethnics, level of education, employment, dependent variable was adherence to take ARV, analysis logistic regression with SPSS 16.0. α (0,05). The result that level of education (p=0.999; prevalence ratio (PR)=0.000), duration of ARV treatment (p=0.210; PR=2.988), level of knowledge (p=0.001; PR=4.450. It means only level of knowledge can increase 4.450 times of adherence to take ARV. The conclution wasrespondent that have good level of knowledge about antiretroviral schedule, risk, side effect, benefit, and outcomesinfluence adherence to take ARV than they have low level of knowledge. Health worker in Clinic VCT should conduct to increase counselling quality by giving about risk of ARV treatment, side effect, and all about ARV treatment and need social support to make them adhere to take ARV as doctors instruction.