Belowground changes to community structure alter methane-cycling dynamics in Amazonia

Meyer, Kyle; Morris, Andrew H.; Webster, Kevin D.; Klein, Ann M.; Kroeger, Marie E.; Meredith, Laura; Brændholt, Andreas; Nakamura, Fernanda Mancini; Venturini, Andressa Monteiro; Souza, Leandro Fonseca de; Shek, Katherine L.; Danielson, Rachel E.; Haren, Joost van; Camargo, Plínio Barbosa de; Tsai, Siu Mui; Dini-Andreote, Fernando; Mauro, Jose M.S. de; Barlow, Jos; Berenguer, Erika; Nüsslein, Klaus; Saleska, S. R.; Rodrigues, Jorge L. Mazza; Bohannan, Brendan J. M.

doi:10.1016/j.envint.2020.106131

Cited by 25 publications

(39 citation statements)

References 82 publications

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“…This forest-to-pasture conversion affects soil methane cycling, where forest soils that were previously acting as a methane sink now become sources of methane (Fernandes et al, 2002). This study confirms previous field results (Meyer et al, 2020;Fernandes et al, 2002;Goreau;De Mello, 1988;Steudler et al, 1996), although the values obtained in our experiments cannot be directly compared to those reported, since the results are limited to a 10 cm surface layer of soil. The trend observed is the same recorded by Steudler et al (1996), in that forests consume 2.74 more methane than pastures.…”

Section: Discussionsupporting

confidence: 87%

“…DNA was extracted for molecular analyses from greenhouse soils that originated in Ariquemes and Belterra (Tapajós region) and from soils of the field study in the Tapajós regions. Total DNA was extracted from soil samples using the PowerLyzer PowerSoil DNA Isolation Kit (Qiagen, Hilden, Germany) from 250 mg of soil, according to the protocol provided by the manufacturer, except that after adding solution C1 the stirring time was extended to 15 minutes followed by 3 min centrifugation (Venturini et al, 2020). The amount and quality of the DNA extracted was analyzed in a Nanodrop 2000c spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) at an optical density of 260 nm.…”

Section: Dna Extractionmentioning

confidence: 99%

“…The establishment of pasture lands is the main cause of deforestation in the Amazon region (Dias et al, 2016;Margulis, 2003). This transformation of rainforest into pastures leads to the net increased emission of the powerful greenhouse gas methane, and turns a methane consuming forest soil into a methane producing pasture soil (Meyer et al, 2020;Fernandes et al, 2002;Steudler et al, 1996;Goreau;De Mello, 1988). The resulting greenhouse gas emissions account for half of Brazil's greenhouse gas production, and have already exceeded national emissions from fossil fuel by more than 20% (Bustamante et al, 2012;Fearnside & Imbrozio Barbosa, 1998).…”

Section: Introductionmentioning

confidence: 99%

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Maintaining grass coverage increases methane uptake in Amazonian pasture soils

Souza

Obregón

Domeignoz‐Horta

et al. 2021

Preprint

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Cattle ranching is the largest driver of deforestation in the Brazilian Amazon. The rainforest- to-pasture conversion affects the methane cycle in upland soils, changing it from sink to source of atmospheric methane. However, it remains unknown if management practices could reduce the impact of land-use on methane cycling. In this work, we evaluated how pasture management can regulate the soil methane cycle either by maintaining continuous grass coverage on pasture soils, or by liming the soil to amend acidity. Methane fluxes from forest and pasture soils were evaluated in moisture-controlled greenhouse experiments with and without grass cover (Urochloa brizantha cv. Marandu) or liming. In parallel, we assessed changes in the soil microbial community structure of both bare pasture soil as well as rhizosphere soil through high throughput sequencing of the 16S rRNA gene, and quantified the methane cycling microbiota by their respective marker genes related to methane generation (mcrA) or oxidation (pmoA). The experiments used soils from eastern and western Amazonia, and concurrent field studies allowed us to confirm greenhouse data. The presence of a grass cover not only increased methane uptake by up to 35% in pasture soils, but also reduced the abundance of the methane-producing community. In the grass rhizosphere this reduction was up to 10-fold. Methane-producing archaea belonged to the genera Methanosarcina sp., Methanocella sp., Methanobacterium sp., and Rice Cluster I. Further, we showed that liming compromised the capacity of forest and pasture soils to be a sink for methane, and instead converted formerly methane-consuming forest soils to become methane sources in only 40-80 days. Our results demonstrate that pasture management that maintains grass coverage can mitigate soil methane emissions, if compared to a bare pasture soil.

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Section: Discussionsupporting

confidence: 87%

Section: Dna Extractionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Maintaining grass coverage increases methane uptake in Amazonian pasture soils

Souza

Obregón

Domeignoz‐Horta

et al. 2021

Preprint

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“…The transfer of materials by the Amazon River to sediments at sites FP2 and FP3 was indicated by their higher contents of trace metals, such as Cu, Mn, Fe, and Zn when compared to FP1, which is exposed exclusively to the Tapajós River. In fact, in In the Amazon region, several studies already showed the importance of soil chemical properties on the soil microbial community composition (Rodrigues et al, 2013) and function (Lammel et al, 2015;Meyer et al, 2020;Paula et al, 2014). Here we show that archaeal and bacterial communities in sediments from the three floodplains analysed are significantly distinct from upland forest soil communities regarding their composition.…”

Section: Discussionsupporting

confidence: 48%

“…Floodplains seem to play a significant role in the regional and global C budget (Gedney et al, 2019; Junk, 1997; Moreira‐Turcq et al, 2003; Pangala et al, 2017). While uplands forests are considered important tropical methane (CH 4 ) sinks (Meyer et al, 2020), floodplains represent one of the largest natural sources of CH 4 into the atmosphere (Conrad, 2009; Gedney et al, 2019), including the significant process of CH 4 transfer through trees (Pangala et al, 2017). Modelling studies have predicted that Amazonian floodplains may contribute up to 7% of total global CH 4 emissions (Potter et al, 2014; Wilson et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Not just a methane source: Amazonian floodplain sediments harbour a high diversity of methanotrophs with different metabolic capabilities

et al. 2021

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The Amazonian floodplain forests are dynamic ecosystems of great importance for the regional hydrological and biogeochemical cycles and function as a significant CH 4 source contributing to the global carbon balance. Unique geochemical factors may drive the microbial community composition and, consequently, affect CH 4 emissions across floodplain areas. Here, we report the in situ composition of CH 4 cycling microbial communities in Amazonian floodplain sediments. We considered how abiotic factors may affect the microbial community composition and, more specifically, CH 4 cycling groups. We collected sediment samples during wet and dry seasons from three different types of floodplain forests, along with upland forest soil samples, from the Eastern Amazon, Brazil. We used high-resolution sequencing of archaeal and bacterial 16S rRNA genes combined with real-time PCR to quantify Archaea and Bacteria, as well as key functional genes indicative of the presence of methanogenic (mcrA) and methanotrophic (pmoA) microorganisms. Methanogens were found to be present in high abundance in floodplain sediments, and they seem to resist the dramatic environmental changes between flooded and nonflooded conditions. Methanotrophs known to use different pathways to oxidise CH 4 were detected, including anaerobic archaeal and bacterial taxa, indicating that a wide metabolic diversity may be harboured in this highly variable environment. The floodplain environmental variability, which is affected by the river origin, drives not only the sediment chemistry but also the composition of the microbial communities. These environmental changes seem also to affect the pools of methanotrophs occupying distinct niches. Understanding these shifts in the methanotrophic communities could improve our comprehension of the CH 4 emissions in the region.

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Impacts of land-use change on soil microbial communities and their function in the Amazon Rainforest

Danielson

Rodrigues

2022

Advances in Agronomy

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Belowground changes to community structure alter methane-cycling dynamics in Amazonia

Cited by 25 publications

References 82 publications

Maintaining grass coverage increases methane uptake in Amazonian pasture soils

Maintaining grass coverage increases methane uptake in Amazonian pasture soils

Not just a methane source: Amazonian floodplain sediments harbour a high diversity of methanotrophs with different metabolic capabilities

Impacts of land-use change on soil microbial communities and their function in the Amazon Rainforest

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