Forest ecosystems play a vital role in the earth’s hydrological process, and precipitation intercepted by forests accounts for more than a quarter of the water in the terrestrial hydrologic cycle. However, water retention in the three layers (canopy, litter, and soil) of forest ecosystems has not yet been thoroughly investigated on a global scale. Here, we investigate the global pattern of forest water retention capacity (WRC) and its controlling environmental factors based on 982 observations of 21 controlling factors in the three forest layers, mainly from 1990 to 2018. The results show that global WRC varies among the different forest types and climatic zones with a mean of 456.71 mm, while the average total water storage is 22,662.47 km3 in forest ecosystems. Climatic variables are the leading factors contributing to the variations in forest WRC, followed by forest structure factors, soil properties, terrain factors, and litter factors. This study advances our understanding of the mechanisms underlying large-scale variations in forest WRC in different climate zones and forest types. The findings demonstrate that controlling factors should be considered when developing policy for regions with important ecological functions. They also provide a benchmark to improve ecohydrological models for simulating global WRC.