Benchmarking of commercially available CHO cell culture media for antibody production

Scale-down of CHO cell fed-batch cultures

“…This feeding strategy resulted in overfeeding of amino acids by Actifeed A/B on both clones ( Figure 6) due to an imbalance in the feed. Notably, overfeeding of amino acids also occurred when following the protocols prescribed by the suppliers 47 . This study demonstrates that CHO cells may display a high variation in specific glucose consumption in different basal medium-feed combinations and during different phases.…”

“…Part of the variation in glucose consumption rate may be caused by uncontrolled glucose levels. The high glucose levels were shown to induce inefficient use of glucose, thus result in higher glucose consumption rate and higher lactate production rate 47 . Hence, control of glucose concentration at low values may reduce the variation and keep feeds more balanced throughout the process.…”

“…A feed is generally more concentrated than a basal medium to maximize culture volumetric productivity and product titer. Studies have shown that the composition of culture media and feeds can influence cell growth, protein productivity 44 , gene expression 45 , product quality 10,46,47 , and metabolism of lactate and ammonia 6,45,48,49 .…”

“…For different medium-feed combinations the richness and composition of the basal media and feeds differ widely. The optimal composition of a basal medium or a feed is highly dependent upon the basic type of CHO cell used, specific characteristics of the generated sub-clones, and type of product 44,47 . Thus, there is no single basal medium or feed suitable for all CHO cultures.…”

“…Rodrigues et al 44 have compared viable cell concentration and antibody production in seven commercially available media in batch cultures of a CHO-K1 cell line and stressed the importance of selecting the correct medium for the outcome of a process. Reinhart et al 47 compared cell growth, antibody production, antibody quality on eight chemically defined media for a CHO DG44 cell line in batch, and with a subset of conditions in fed-batch.…”