Bencycloquidium bromide inhibits nasal hypersecretion in a rat model of allergic rhinitis

Long, Rodney; Zhou, Yifei; Huang, Jiangju; Li, Peng; Meng, Long; Zhu, Shirley; Li, Juan

doi:10.1007/s00011-015-0800-6

Cited by 18 publications

(27 citation statements)

References 36 publications

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“…The symptoms and pathological features of AR could be reproduced, at least in part, in its murine models. For example, nasal antigen challenge to immunized mice evoked sneezing and nasal rubbing, as well as infiltration of inflammatory cells into the nasal submucosa in these mice [ 35 – 38 ]. In addition, several murine models exhibiting antigen-induced increase in sneezing response have been developed [ 39 , 40 ], although the mechanistic analysis has been poorly performed in these studies.…”

Section: Discussionmentioning

confidence: 99%

“…The mechanisms of NHR have also been investigated in animal models, employing nasal obstruction and secretion as outcomes. Thus, treatment with a muscarinic M1/M3 antagonist attenuates antigen-induced mucus hypersecretion in rats [ 35 ]. Pharmacological intervention of neurokinin 2 and bradikinin 1/2 receptors inhibits antigen-induced increase in the nasal occlusion response in guinea pigs [ 44 , 45 ].…”

Section: Discussionmentioning

confidence: 99%

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Essential Contribution of CD4+ T Cells to Antigen-Induced Nasal Hyperresponsiveness in Experimental Allergic Rhinitis

et al. 2016

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Nasal hyperresponsiveness (NHR) is a characteristic feature of allergic rhinitis (AR); however, the pathogenesis of NHR is not fully understood. In this study, during the establishment of an experimental AR model using ovalbumin-immunized and -challenged mice, augmentation of the sneezing reaction in response to nonspecific proteins as well as a chemical stimulant was detected. Whether NHR is independent of mast cells and eosinophils was determined by using mast cell- and eosinophil-deficient mice. NHR was suppressed by treatment with anti-CD4 antibody, suggesting the pivotal contribution of CD4+ T cells. Furthermore, antigen challenge to mice to which in vitro-differentiated Th1, Th2, and Th17 cells but not naïve CD4+ T cells had been adoptively transferred led to the development of equivalent NHR. Since antigen-specific IgE and IgG were not produced in these mice and since antigen-specific IgE-transgenic mice did not develop NHR even upon antigen challenge, humoral immunity would be dispensable for NHR. CD4+ T cells play a crucial role in the pathogenesis of AR via induction of NHR, independent of IgE-, mast cell-, and eosinophil-mediated responses.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Essential Contribution of CD4+ T Cells to Antigen-Induced Nasal Hyperresponsiveness in Experimental Allergic Rhinitis

et al. 2016

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“…AR rat models were established as described previously [29] with minor modifications, as summarized in Figure 1. In brief, the rats were intraperitoneally sensitized with 0.3 mg of OVA (albumin egg, Sigma, MO, USA) and 30 mg of Al(OH) 3 (Damao Chemical Reagent Factory, China) dissolved in 1 mL of physiological saline once every other day for 2 weeks.…”

Section: Methodsmentioning

confidence: 99%

Mahuang Fuzi Xixin Decoction Attenuates Th1 and Th2 Responses in the Treatment of Ovalbumin-Induced Allergic Inflammation in a Rat Model of Allergic Rhinitis

Ren

Tang

Chen

et al. 2017

Journal of Immunology Research

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Allergic rhinitis (AR) is one of the most common allergic diseases, which adversely affect patients' quality of life. Mahuang Fuzi Xixin decoction (MFXD) has been widely used to treat AR in clinics in Asian countries. This study investigated the effect and possible therapeutic mechanisms of MFXD in the treatment of AR. A Wistar rat model of ovalbumin- (OVA-) induced AR was established and then treated with three doses of MFXD; AR symptoms, serum total immunoglobulin E, histamine, histopathological features, and release and expression of factors related to type 1 helper T (Th1) and type 2 helper T (Th2) responses were analyzed. Our study demonstrated that MFXD has a good therapeutic effect on OVA-induced allergic inflammation in an AR rat model as manifested in reduced frequencies of sneezing and nasal scratching and in reduced serum levels of total IgE and HIS. In addition, MFXD regulates imbalance in Th1/Th2 cells caused by AR by simultaneously attenuating Th1 and Th2 responses, such as by reducing the serum levels of IFN-γ and IL-4 and mRNA expression levels of IFN-γ, IL-4, GATA-3, and STAT-6. This study provided valuable information on the immunoregulatory effect of MFXD for the treatment of AR in future clinical studies.

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“…According to our previous retrospective study, nasal vidian neurectomy alleviates nasal symptoms and improves the quality of life of patients with refractory AR who failed to respond to various medical treatments, including immunotherapy . In addition, based on accumulating evidence, treatment with a muscarinic antagonist improves the nasal hypersecretion symptoms of patients with AR . Although these clinical studies strongly imply the efficacy of inhibiting nasal parasympathetic or cholinergic nerve hyperactivity in patients with AR, the mechanism underlying the effect of nasal nerve fibers on the immune function of patients with AR remains unclear.…”

mentioning

confidence: 99%

“…6 In addition, based on accumulating evidence, treatment with a muscarinic antagonist improves the nasal hypersecretion symptoms of patients with AR. [7][8][9] Although these clinical studies strongly imply the efficacy of inhibiting nasal parasympathetic or cholinergic nerve hyperactivity in patients with AR, the mechanism underlying the effect of nasal nerve fibers on the immune function of patients with AR remains unclear.…”

mentioning

confidence: 99%

Cholinergic neuron‐like D‐U87 cells promote polarization of allergic rhinitis T‐helper 2 cells

Wang

Xia

et al. 2019

Int Forum Allergy Rhinol

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Background Parasympathetic nerve hypersensitivity contributes to the severity of allergic rhinitis (AR), but the precise mechanism underlying neuroimmune regulation in patients with AR remains unclear. This study investigated the effect of cholinergic nerve inhibition on AR CD4+ T‐helper (Th)2‐cell polarization and the underlying regulatory mechanism in vitro. Methods An in‐vitro neuroimmune coculture model of D‐U87 cells and CD4+ T cells was established. D‐U87 cells with cholinergic neuron characteristics were used as cholinergic neuron models. CD4+ T cells were derived from peripheral blood monocytes from AR patients (n = 60) and control subjects (n = 40). Th1‐ and Th2‐cell percentages were measured by flow cytometry. Proteins involved in related signaling pathways were analyzed by protein chip assay and Western blotting. Results The Th2‐cell percentage among CD4+ T cells from AR patients was significantly increased after coculture with D‐U87 cells and was decreased by ipratropium bromide (IB) treatment. In contrast, the Th1‐cell percentage among control CD4+ T cells was significantly increased after coculture with D‐U87 cells, but was unaltered by IB treatment. Furthermore, phosphorylated Akt (p‐Akt) protein levels increased in CD4+ T cells from both controls and AR patients after coculture with D‐U87 cells and decreased after IB treatment. However, higher p‐Akt levels were observed in cells from AR patients than in cells from control subjects. Moreover, Akt inhibition decreased Th2‐cell percentage in AR patients. Conclusion In‐vitro cholinergic nerve inhibition with IB decreased AR CD4+ T‐cell polarization into Th2 cells partially through an Akt‐dependent mechanism.

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Bencycloquidium bromide inhibits nasal hypersecretion in a rat model of allergic rhinitis

Abstract: BCQB attenuates mucus hypersecretion in AR, possibly involving in the NF-κB signaling pathway.

Cited by 18 publications

References 36 publications

Essential Contribution of CD4+ T Cells to Antigen-Induced Nasal Hyperresponsiveness in Experimental Allergic Rhinitis

Essential Contribution of CD4+ T Cells to Antigen-Induced Nasal Hyperresponsiveness in Experimental Allergic Rhinitis

Mahuang Fuzi Xixin Decoction Attenuates Th1 and Th2 Responses in the Treatment of Ovalbumin-Induced Allergic Inflammation in a Rat Model of Allergic Rhinitis

Cholinergic neuron‐like D‐U87 cells promote polarization of allergic rhinitis T‐helper 2 cells

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