Bending rigidity of phosphatidylserine-containing lipid bilayers in acidic aqueous solutions

Mitkova, Denitsa; Marukovich, Natalia; Ermakov, Yury A.; Vitkova, Victoria

doi:10.1016/j.colsurfa.2013.12.059

Cited by 11 publications

(9 citation statements)

References 33 publications

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“…Experimental data, albeit limited, 22 in general support the theoretical results. Of particular interest is the variation of the bending rigidity with surface charge, however, this problem has been investigated either only for relatively low fractions of the ionic species (lipids or surfactants) [23][24][25][26][27][28] or on purely ionic lyotropic systems. 29,30 Giant unilamellar vesicles (GUVs) represent a suitable system for systematic measurements on membranes with good control of the composition.…”

Section: Introductionmentioning

confidence: 99%

“…Membranes made of 1-stearoyl-2-oleoyl-snglycero-3-phosphatidylcholine (SOPC) and 1-palmitoyl-2-oleoylsn-glycero-3-phosphatidyl-L-serine (sodium salt) (POPS) show negligible stiffening for up to a 16% POPS molar fraction. Using fluctuation spectroscopy of giant unilamellar vesicles, Rowat et al 26 detected a modest increase of 3-5 k B T in the bending rigidity for a membrane system of dimyristoyl phosphatidylcholine with 1-5 mol% ionic surfactants, and Mitkova et al 27 measured an increase of 7 k B T for a 20% fraction of negatively charged lipids in membranes made of SOPC:DOPS (dioleolylphosphatidylcholine) in acidic solutions. Membranes containing larger fractions of charged lipids (up to 40%) were studied by Vitkova et al 28 Micropipette aspiration of giant vesicles made of POPC:POPS mixtures showed an increase in membrane bending rigidity by 10 k B T. However, these experiments were conducted in a high sucrose concentration (0.17 M sucrose), at which sugars bind quite strongly to a lipid bilayer, resulting in membrane lateral expansion and thinning.…”

Section: Introductionmentioning

confidence: 99%

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Bending rigidity of charged lipid bilayer membranes

et al. 2019

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bending rigidity of charged lipid bilayer membranes

et al. 2019

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“…Bending elasticity is the mechanical parameter that characterizes the membrane resistance to local curvature changes induced by external stresses as small as Brownian motion. It is strongly dependent on subtle modifications of the environment characteristics like a pH shift (Méléard, et al, 1998;Mitkova, et al, 2014), a change in temperature (Fernandez-Puente, et al, 1994;Méléard, et al, 1997) or aqueous salinity, the chosen buffer and its concentrations (Méléard, et al, 1998). Naturally, bending elasticity is influenced by the lipid composition of the bilayer (Fernandez-Puente, et al, 1994;Méléard, et al, 1997), sometimes simply related to the hydrophobic thickness (Fernandez-Puente, et al, 1994), sometimes in a more unexpected manner as shown in the case of photo-induced lipid peroxidation (Bouvrais, et al, 2010;Méléard, et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Melittin modifies bending elasticity in an unexpected way

Pott¹,

Gerbeaud²,

Barbier³

et al. 2015

Chemistry and Physics of Lipids

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Understanding the molecular mechanism of the interaction of amphipathic and antimicrobial peptides with membranes is of fundamental interest, especially because of the potential of amphipathic peptides as therapeutics. The most studied amphipathic peptides in this context are certainly melittin, magainin and alamethicin, of which melittin is the only one to exhibit a powerful hemolytic and therefore toxic action. Herein we study the effect of the antimicrobial but hemolytic peptide melittin on the bending elasticity of giant unilamellar vesicles (GUVs). The results are compared to the effects of non-hemolytic amphipathic peptides such as alamethicin. We found that monomeric melittin acts very differently on the membrane mechanical properties. Strikingly, the difference is the most pronounced for low peptide concentrations, relevant for the hemolytic action. This gives some insight into the subtle nature of this peptide-membrane interaction. Furthermore, the results show that bending elasticity measurements might be a sensitive way to distinguish between lytic and non-lytic antimicrobial peptides.

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“…Concomitant with the increase of order, the elasticity of the bilayer decreases [4]. A number of experimental studies on the bending stiffness of lipid membranes were performed [30,31,32], illustrating, e.g., the influence of the electrostatic characteristics of the membrane-water interface [30,32] or membrane-active compounds [31] on membrane mechanical properties. Membrane-water interfacial electrostatic properties are commonly described by the zeta potential, and it was found [30] that the relation between zeta potential and bending stiffness is qualitatively captured by the theoretical model of Helfrich and Winterhalter [33,34].…”

Section: Introductionmentioning

confidence: 99%

Effect of Sodium and Chloride Binding on a Lecithin Bilayer. A Molecular Dynamics Study

2017

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The effect of ion binding on the structural, mechanical, dynamic and electrostatic properties of a 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) bilayer in a 0.5 M aqueous NaCl solution is investigated using classical atomistic molecular dynamics simulation with different force-field descriptions for ion-ion and ion-lipid interactions. Most importantly, the repulsive Lennard–Jones parameters for the latter were modified, such that approximately similar binding of cations and anions to the lipid membrane is achieved. This was done to qualitatively improve the apparent ion-lipid binding constants obtained from simulations with the original force field (Berger lipids and GROMOS87 ions in combination with the SPC water model) in comparison to experimental data. Furthermore, various parameters characterizing membrane structure, elasticity, order and dynamics are analyzed. It is found that ion binding as observed in simulations involving the modified in comparison to the original force-field description leads to: (i) a smaller salt-induced change in the area per lipid, which is in closer agreement with the experiment; (ii) a decrease in the area compressibility and bilayer thickness to values comparable to a bilayer in pure water; (iii) lipid deuterium order parameters and lipid diffusion coefficients on nanosecond timescales that are very similar to the values for a membrane in pure water. In general, salt effects on the structural properties of a POPC bilayer in an aqueous sodium-chloride solution appear to be reproduced reasonably well by the new force-field description. An analysis of membrane-membrane disjoining pressure suggests that the smaller salt-induced change in area per lipid induced by the new force-field description is not due to the alteration of membrane-associated net charge, but must rather be understood as a consequence of ion-specific effects on the arrangement of lipid molecules.

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Bending rigidity of phosphatidylserine-containing lipid bilayers in acidic aqueous solutions

Cited by 11 publications

References 33 publications

Bending rigidity of charged lipid bilayer membranes

Bending rigidity of charged lipid bilayer membranes

Melittin modifies bending elasticity in an unexpected way

Effect of Sodium and Chloride Binding on a Lecithin Bilayer. A Molecular Dynamics Study

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