Beneficial Effect of a Fermented Wheat Germ Extract in Intestinal Epithelial Cells in case of Lipopolysaccharide-Evoked Inflammation

Karancsi, Zita; Móritz, Alma Virág; Lewin, Nancy E.; Veres, Ágota; Jerzsele, Ákos; Farkas, Orsolya

doi:10.1155/2020/1482482

Cited by 11 publications

(8 citation statements)

References 34 publications

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“…Both 1% and 2% FWGE significantly decreased the ROS after a 24 h treatment. These findings are in good correlation with Karancsi et al [ 42 ], who elucidated firstly beneficial effects of FWGE in case of LPS-evoked oxidative damage. FWGE could decrease excessive intracellular ROS levels after LPS administration and exerted protective effect on the integrity of the IPEC-J2 cell monolayer exposed to LPS treatment.…”

Section: Discussionsupporting

confidence: 90%

The Impact of Fermented Wheat Germ Extract on Porcine Epithelial Cell Line Exposed to Deoxynivalenol and T-2 Mycotoxins

Pomothy

Pászti-Gere

Barna

et al. 2020

Oxidative Medicine and Cellular Longevity

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The effect of fermented wheat germ extract (FWGE) (Immunovet®) was evaluated with cotreatments with deoxynivalenol (DON) and T-2 toxin (T-2). These mycotoxins are produced by Fusarium mold species. The effects of FWGE on IPEC-J2 with DON and T-2 have not been studied until now. The IPEC-J2 porcine, nontumorigenic cell line was selected to investigate the outcome of the individually and simultaneously added compounds, as it has in vivo-like properties. The cells were treated for 24 h with the selected solutions; then, the IPEC-J2 cells were allowed to regenerate in a culture medium for an additional 24 h. In our results, DON and T-2 significantly increased the adverse impacts on cell viability and integrity of the cell monolayer. To elucidate the extent of oxidative stress, extracellular H2O2 concentrations and intracellular reactive oxygen species (ROS) were measured. FWGE appeared to be beneficial to IPEC-J2 cells given the separately and significantly decreased ROS levels. 1% and 2% FWGE could significantly reduce mycotoxin-induced oxidative stress. In conclusion, the results demonstrate that FWGE exerted protective effects to counteract the oxidative stress-provoking properties of applied fusariotoxins in the nontumorigenic IPEC-J2 cell line.

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Section: Discussionsupporting

confidence: 90%

The Impact of Fermented Wheat Germ Extract on Porcine Epithelial Cell Line Exposed to Deoxynivalenol and T-2 Mycotoxins

Pomothy

Pászti-Gere

Barna

et al. 2020

Oxidative Medicine and Cellular Longevity

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“…This noncancerous cell line more closely mimics the human physiology than other tumorigenic or transformed cell lines. Therefore, IPEC-J2 cells are a useful tool with which to investigate the status of the antioxidant defense system, inflammation, or epithelial integrity [24][25][26][27]. These intestinal cells express and produce different inflammatory interleukins (e.g., IL-6, IL-8), cytokines (e.g., granulocyte macrophage colony stimulating factor, TNF-α), defensins, toll-like receptors, and mucins IPEC-J2 cells are intestinal columnar epithelial cells of mid-jejunum that are isolated from neonatal unsuckled piglets [23].…”

Section: Introductionmentioning

confidence: 99%

The Impact of Quercetin and Its Methylated Derivatives 3-o-Methylquercetin and Rhamnazin in Lipopolysaccharide-Induced Inflammation in Porcine Intestinal Cells

et al. 2022

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Oxidative stress in the small intestine can lead to inflammation and barrier malfunction. The present study describes the effect of quercetin (Q), 3-o-methylquercetin (QM), and rhamnazin (R) on cell viability, paracellular permeability, production of intracellular reactive oxygen species (ROS), extracellular hydrogen peroxide (H2O2), and interleukin-6 (IL-6) after challenging jejunal cells (IPEC-J2) with different types (Salmonella enterica ser. Typhimurium, Escherichia coli O111:B4, and E. coli O127:B8) of lipopolysaccharides (LPS) applied in 10 µg/mL concentration. The intracellular ROS level increased after all LPS treatments, which could be decreased by all tested flavonoid compounds in 50 µM concentration. Extracellular H2O2 production significantly increased after Q and R treatment (50 µM). S. Typhimurium LPS could significantly increase IL-6 production of enterocytes, which could be alleviated by Q, QM, and R (50 µM) as well. Using fluorescein isothiocyanate dextran (FD4) tracer dye, we could demonstrate that S. Typhimurium LPS significantly increased the permeability of the cell layer. The simultaneous treatments of S. Typhimurium LPS and the flavonoid compounds showed no alteration in FD4 penetration compared to untreated cells. These results highlight that Q, QM, and R are promising substances that can be used to protect intestinal epithelial cells from the deteriorating effects of oxidative stress.

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“…To determine the interleukin production of cells affected by bacteria and GSOPs, cells were cultured on 6-plate wells and the previously detailed experimental settings were fol-lowed. Samples were taken from the cell supernatants 6 h after the end of treatments [ 27 , 57 , 58 ] for IL-6 and IL-8 measurement with porcine-specific IL-6 and IL-8 ELISA kits according to the instructions of the manufacturer. At the end of the protocol, absorbance measurement of the samples was performed with SpectraMax iD3 (on 450 nm).…”

Section: Methodsmentioning

confidence: 99%

Protective Effects of Grape Seed Oligomeric Proanthocyanidins in IPEC-J2–Escherichia coli/Salmonella Typhimurium Co-Culture

et al. 2022

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Intestinal epithelium provides the largest barrier protecting mammalian species from harmful external factors; however, it can be severely compromised by the presence of bacteria in the gastrointestinal (GI) tract. Antibiotics have been widely used for the prevention and treatment of GI bacterial infections, leading to antimicrobial resistance in human and veterinary medicine alike. In order to decrease antibiotic usage, natural substances, such as flavonoids, are investigated to be used as antibiotic alternatives. Proanthocyanidins (PAs) are potential candidates for this purpose owing to their various beneficial effects in humans and animals. In this study, protective effects of grape seed oligomeric proanthocyanidins (GSOPs) were tested in IPEC-J2 porcine intestinal epithelial cells infected with Escherichia coli and Salmonella enterica ser. Typhimurium of swine origin. GSOPs were able to alleviate oxidative stress, inflammation and barrier integrity disruption inflicted by bacteria in the co-culture. Furthermore, GSOPs could decrease the adhesion of both bacteria to IPEC-J2 cells. Based on these observations, GSOPs seem to be promising candidates for the prevention and treatment of gastrointestinal bacterial infections.

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Beneficial Effect of a Fermented Wheat Germ Extract in Intestinal Epithelial Cells in case of Lipopolysaccharide-Evoked Inflammation

Cited by 11 publications

References 34 publications

The Impact of Fermented Wheat Germ Extract on Porcine Epithelial Cell Line Exposed to Deoxynivalenol and T-2 Mycotoxins

The Impact of Fermented Wheat Germ Extract on Porcine Epithelial Cell Line Exposed to Deoxynivalenol and T-2 Mycotoxins

The Impact of Quercetin and Its Methylated Derivatives 3-o-Methylquercetin and Rhamnazin in Lipopolysaccharide-Induced Inflammation in Porcine Intestinal Cells

Protective Effects of Grape Seed Oligomeric Proanthocyanidins in IPEC-J2–Escherichia coli/Salmonella Typhimurium Co-Culture

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