Beneficial Effect of Copper Supplementation on Deposition of Fluoride in Bone in Fluoride- and Molybdenum-Fed Rabbits

Abstract

Fluorosis is a major public health problem in the world, including India. The present study was undertaken to investigate the role of molybdenum (Mo) in the deposition of fluoride (F) in bone and whether copper (Cu) supplementation has any alleviating role when F and Mo are ingested together. For this purpose, four groups of rabbits were used [control (C), fluoride (F), fluoride + molybdenum (F + Mo), and fluoride + molybdenum + copper (F + Mo + Cu)] to find out the effect of these treatments on various bone-related parameters like intact parathyroid hormone (iPTH), alkaline phosphatase and Cu in serum, hydroxyproline and calcium (Ca) in urine, and minerals (F, Cu, manganese, and zinc) in femur bone ash. Bone mineral content (BMC), bone mineral density (BMD) [by dual energy X-ray absorptiometry (DXA)], and strength of femur bones were also assessed. F content in the femur was significantly higher (P < 0.01) in all experimental groups compared to control group. Mo supplementation increased F deposition in femur bone in the F + Mo group, whereas supplementation of Cu reduced F deposition in the F + Mo + Cu group compared to the F + Mo and F groups. Levels of Cu in femurs of the F + Mo and F + Mo + Cu groups were significantly higher (P < 0.05, P < 0.01, respectively) than in the C group, although serum Cu was significantly lower in the F and F + Mo than the C and F + Mo + Cu groups. Magnesium levels in the F + Mo group were significantly higher (P < 0.05) than in the F and F + Mo + Cu groups. Cu supplementation in the F + Mo + Cu group increased deposition of zinc significantly (P < 0.05) compared to the F and F + Mo groups. Serum iPTH, alkaline phosphatase, and urinary hydroxyproline and Ca were significantly higher (P < 0.01) in the F and F + Mo than in the C and F + Mo + Cu groups. However, serum iPTH and urinary hydroxyproline were higher in the F + Mo group than the F group. Alkaline phosphatase was significantly higher in the F + Mo group than the F and F + Mo + Cu groups. Levels of serum Cu in the F and F + Mo groups were lower than in the C group, though serum Cu was significantly higher in the F + Mo + Cu than in all other groups. DXA analysis of femur bone indicated that BMD in the F + Mo group was significantly higher than in the F (P < 0.05), C (P < 0.01), and F + Mo + Cu (P < 0.05) groups. However, there was no significant difference in BMC among the groups. Bone strength was significantly higher (P < 0.05) in the F + Mo group than in the C group. Results of the present study show that ingestion of Mo with F does not create secondary Cu deficiency (due to increased excretion of Cu through urine). However, Cu concentration was decreased in serum in this group (F + Mo) compared to the C and F + Mo + Cu groups. Deposition of F in femur bone was more (22%) when it was given along with Mo compared to F alone, while F deposition in femur bone was less in the F + Mo + Cu group by 80% compared to the F group. Also, deposition of F in the F + Mo + Cu group was 120% less compared to the F + Mo group. The increas...

Level of Certain Micro and Macro Minerals in Blood of Cattle from Fluoride Polluted Localities of Udaipur, India

“…Increased utilization of zinc and copper for counteracting fluoride-induced oxidative stress may also be, at least partially, responsible for decrease in blood level of these elements. This is substantiated by the observation that supplementation of Zn, Cu and other antioxidant minerals like selenium have beneficial effects in fluoride intoxication (Liu and Kang 2003;Han-Bo et al 2001;Khandare et al 2005b). Bhargavi et al (2004) reported that lower concentration of Ca, Cu and Mg in drinking water was associated with increased prevalence of dental fluorosis in man.…”

Qualidade seminal e histomorfometria dos órgãos reprodutivos de ovinos tratados com fluoreto de sódio

“…Uma vez que não houve incremento na peroxidação lipídica, também não houve necessidade de uma resposta adaptativa por parte da GS. Segundo CHLUBEK (2003) KHANDARE et al (2005), analisando amostras sangüíneas de coelhos tratados com 150ppm de F na água de beber, observaram decréscimo do teor de Cu. Ambos os elementos analisados (Cu e Zn) são essenciais para a formação da enzima antioxidante superóxido dismutase (SOD).…”

Ameliorative effects of boron on serum profile in buffalo (Bubalus bubalis) fed high fluoride ration

“…Fluorosis is a common health problem in animals as well as in human being in the world (Khandare et al 2005). Now a day, industrial fluorosis has become very common due to intensive production systems, mechanization in agriculture and industrialization, that leads to pollution of water and vegetation with fluoride (Chinoy 1993).…”