Beneficial effect of Bidens pilosa L. (Asteraceae) in a rat model of colitis

Abstract

<jats:title>Abstract</jats:title><jats:sec id="j_jbcpp-2019-0166_abs_001_w2aab3b7b7b1b6b1aab1c12b2Aa"><jats:title>Background</jats:title><jats:p><jats:italic>Bidens pilosa</jats:italic> (BP) possessed anti-inflammatory, antioxidant, and immunomodulatory activities. Its beneficial effects on intestinal inflammation and oxidative stress in 2,4,6 trinitrobenzene sulfonic acid (TNBS) induced colitis in Wistar rats was evaluated.</jats:p></jats:sec><jats:sec id="j_jbcpp-2019-0166_abs_002_w2aab3b7b7b1b6b1aab1c12b3Aa"><jats:title>Methods</jats:title><jats:p>Thirty female Wistar rats weighing 180–200 g were distributed into six groups (n = 5): non-colitic, untreated colitic and colitic rats treated graded doses of methanol extract of BP (50–400 mg/kg). Colitis was induced in rats by intracolonic instillation of 0.2 mL of 40 mg/mL TNBS. BP was administered two days pre-colitis induction and treatments continued until seven days post-colitis induction. A day after the last treatment, rats were euthanized, colon removed aseptically and response to treatment assessed. Phytochemical composition of BP was determined using the GC-MS.</jats:p></jats:sec><jats:sec id="j_jbcpp-2019-0166_abs_003_w2aab3b7b7b1b6b1aab1c12b4Aa"><jats:title>Results</jats:title><jats:p>BP significantly reduced macroscopic colonic damage score, weight/length ratio, colonic lipid peroxidation level, leukocytes infiltration, and TNF-<jats:italic>α</jats:italic> level in comparison to untreated colitic rats (p ≤ 0.008). Similarly, treatment with 200 and 400 mg/kg BP prevented depletion of colonic glutathione level than other treatment groups (p ≤ 0.0002). Histological findings revealed that treatment with 400 mg/kg BP significantly preserved the mucosal epithelial layer. It also prevented ulceration and sloughing of the mucosal layers and reduced infiltration of inflammatory cells compared to other treatment groups. Among the 16 compounds identified were oleic acid (6.2%) and <jats:italic>n-</jats:italic>hexadecanoic acid (2.0%) with antioxidant anti-inflammatory activities.</jats:p></jats:sec><jats:sec id="j_jbcpp-2019-0166_abs_004_w2aab3b7b7b1b6b1aab1c12b5Aa"><jats:title>Conclusions</jats:title><jats:p>The beneficial effects of BP in rat colitis might be related to the reduction of leucocytes infiltration, inhibition of oxidative stress and pro-inflammatory cytokines.</jats:p></jats:sec>

Anticoccidial activity of natural plants extracts mixture against Eimeria tenella: An in vitro and in vivo study

“…Our results showed that when the eight plant extracts and essential oils were mixed, the inhibitory effect of the EM on ETS invasion was more pronounced. Previous studies have shown that PGE, PAE, AAE, PMPE, POE, and AAO have hemostatic or therapeutic effects on hematochezia ( 11 , 12 , 14 – 17 ), and BPE, AAE, PMPE, AAO, and CSO have therapeutic or anti-inflammatory effects on enteritis ( 13 – 15 , 17 , 18 ). Therefore, studies exploring whether mixing the extracts and essential oils could have a synergistic effect, which better explains the observation that the EM more strongly inhibits ETS invasion than the individual extracts and essential oils, are needed.…”

“…For example, Punica granatum L. extract (PGE) is used for deworming, to treat abdominal pain caused by worm infections, and to treat diarrhea and bleeding ( 11 ). While Plantago asiatica L. extract (PAE) can be used to treat diarrhea, dysentery, and hematuria (blood in the urine) ( 12 ), Bidens pilosa L. extract (BPE) may help treat enteritis ( 13 ), Acalypha australis L. extract (AAE) acts as an astringent hemostatic and helps treat enteritis, hematuria, and hematochezia (bloody stools) ( 14 ), and Pteris multifida Poir extract (PMPE) helps to stop bleeding and can be used to treat enteritis, hematuria, and hematochezia ( 15 ). Furthermore, Portulaca oleracea L. sp.…”

Bidens pilosa Linn.: Beautiful Weed for the Healthy Mouth – A Mini Review

“…The alcoholic extracts inhibited the growth of Grampositive and Gram-negative bacteria [16,17,19,38]; even toward drug resistance bacteria [21], and Mycobacterium [17]; yeast, and fungi [3,16,17,19]. Other effects are also proven, including analgesic activity [18]; anti-inflammatory [3,13,14]; antioxidants [3,13,19,22]; antiplasmodial activity [3,15]; larvicidal activity [19]; haematopoietic strength and modulator of white blood cell differential count [3,12]; anticancer and antipyretic activity [3,19,20]; antiallergic activity, hypotensive effects, gastric antisecretory and antiulcer activities [3].…”

“…The B. pilosa plant is widely used traditionally for treating inflammation [2]. The anti-inflammatory effect has been proven in laboratory animals of colitis [13], or induced inflamed by chemical and mechanical [80], induced gastric ulcer, liver inflammation, or arthritis-type swelling [9]. The analgesic effect of the plant was proven to reduce oral paint of HIV/AIDS patients [18].…”

“…The properties are no longer folklores because many studies have been proven the activities in vitro, in vivo, and ex vivo assays [3,7,9,[12][13][14][15][16][17][18][19][20][21][22][23]. Currently, the plant become a market commodity, such as dried plant powder, B. pilosa tea, extracts, and tinctures.…”