Beneficial effect of pharmacological mobilization of bone marrow in experimental cerebral ischemia

Six, Isabelle; Gasan, Gaëlle; Mura, Emmanuelle; Bordet, Régis

doi:10.1016/s0014-2999(02)02785-1

Cited by 107 publications

(86 citation statements)

References 8 publications

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“…Along with other growth factors (Schabitz et al, 2001(Schabitz et al, , 2003, G-CSF has been previously reported to reduce lesion volume after ischemia in mice (Six et al, 2003) and the rat (Schabitz et al, 2003). In these studies, 50 or 60 mg/kg G-CSF was administered via a single subcutaneous injection, and their findings formed the basis for the present study.…”

Section: Discussionmentioning

confidence: 93%

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G-CSF Reduces Infarct Volume and Improves Functional Outcome after Transient Focal Cerebral Ischemia in Mice

Gibson

Bath

Murphy

2005

J Cereb Blood Flow Metab

156

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Growth factors possess neuroprotective and neurotrophic properties in vitro, but few have been extensively studied in vivo after stroke. In the present study, we investigated the potential functional benefits of granulocyte colony-stimulating factor (G-CSF) administration after focal cerebral ischemia. Male mice underwent 60-minute middle cerebral artery occlusion (MCAO) and received G-CSF (50 lg/kg, subcutaneously) or vehicle (saline) at the onset of reperfusion. Granulocyte colony-stimulating factor-treated mice killed at 48 hours after MCAO revealed a 445% reduction (Po0.05) in lesion volume. In terms of body weight recovery, and in tests of motor (grid test and rotarod) and cognitive ability (water maze), MCAO significantly worsened the outcome in vehicletreated mice as compared with shams (Po0.05). However, G-CSF treatment was beneficial as, compared with vehicle, this significantly improved weight recovery and motor ability. This effect was most apparent on the water maze where G-CSF-treated mice were indistinguishable from shams in terms of acquiring the task. These results indicate long-term beneficial effects of a single dose of G-CSF administered on reperfusion, and illustrate the need to further investigate the mechanisms of G-CSF action.

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Section: Discussionmentioning

confidence: 93%

“…After focal cerebral ischemia, G-CSF administration has been shown to result in significant decreases in lesion volume, both in mice (Six et al, 2003) and the rat (Schabitz et al, 2003). However, these studies have only looked at short-term effects, giving little indication of whether lesion development was prevented or simply delayed.…”

Section: Introductionmentioning

confidence: 99%

G-CSF Reduces Infarct Volume and Improves Functional Outcome after Transient Focal Cerebral Ischemia in Mice

Gibson

Bath

Murphy

2005

J Cereb Blood Flow Metab

156

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“…Moreover, treatments with diVerent classes of COX inhibitors had negative eVects on the proliferation of neuronal progenitor cells in dentate gyrus following cerebral ischemia [120,121]. Finally, recent studies demonstrated beneWcial eVects of pro-inXammatory cytokines, a granulocyte-macrophage colony-stimulating factor (GM-CSF) and macrophage colony stimulating factor (MCSF) in the treatment of experimental cerebral ischemia [79,122,127].…”

Section: Inxammatory Response After Stroke: a Dual Role?mentioning

confidence: 99%

Inflammation, plasticity and real-time imaging after cerebral ischemia

2009

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With an incidence of approximately 350 in 100,000, stroke is the third leading cause of death and a major cause of disability in industrialized countries. At present, although progress has been made in understanding the molecular pathways that lead to ischemic cell death, the current clinical treatments remain poorly eVective. There is mounting evidence that inXammation plays an important role in cerebral ischemia. Experimentally and clinically, brain response to ischemic injury is associated with an acute and prolonged inXammatory process characterized by the activation of resident glial cells, production of inXammatory cytokines as well as leukocyte and monocyte inWltration in the brain, events that may contribute to ischemic brain injury and aVect brain recovery and plasticity. However, whether the post-ischemic inXammatory response is deleterious or beneWcial to brain recovery is presently a matter of debate and controversies. Here, we summarize the current knowledge on the molecular mechanisms underlying post-ischemic neuronal plasticity and the potential role of inXammation in regenerative processes and functional recovery after stroke. Furthermore, because of the dynamic nature of the brain inXammatory response, we highlight the importance of the development of novel experimental approaches such as real-time imaging. Finally, we discuss the novel transgenic reporter mice models that have allowed us to visualize and to analyze the processes such as neuroinXammation and neuronal repair from the ischemic brains of live animals.

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“…Data on vital status was available in 6 studies (Gibson et al, 2005a;Schabitz et al, 2003;Schneider et al, 2005;Sevimli et al, 2009;Six et al, 2003;Yanqing et al, 2006); G-CSF reduced the odds of dying almost 4-fold (OR 0.27, 95% CI 0.14 to 0.51, p<0.0001).…”

Section: Survivalmentioning

confidence: 99%

Granulocyte-colony stimulating factor in experimental stroke and its effects on infarct size and functional outcome: A systematic review

England

Gibson

Bath

2009

Brain Research Reviews

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Beneficial effect of pharmacological mobilization of bone marrow in experimental cerebral ischemia

Cited by 107 publications

References 8 publications

G-CSF Reduces Infarct Volume and Improves Functional Outcome after Transient Focal Cerebral Ischemia in Mice

G-CSF Reduces Infarct Volume and Improves Functional Outcome after Transient Focal Cerebral Ischemia in Mice

Inflammation, plasticity and real-time imaging after cerebral ischemia

Granulocyte-colony stimulating factor in experimental stroke and its effects on infarct size and functional outcome: A systematic review

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