Beneficial Effects of an Amino Acid Mixture on Colonic Mucosal Healing in Rats

Liu, Xinxin; Beaumont, Martín; Walker, Francine; Chaumontet, Catherine; Andriamihaja, Mireille; Matsui, Hideki; Khodorova, Nadezda; Lan, Annaïg; Gaudichon, Claire; Bénamouzig, Robert; Tomé, Daniel; Davila, Anne‐Marie; Marie, Jean‐Claude; Blachier, François

doi:10.1097/01.mib.0000435849.17263.c5

Cited by 22 publications

(15 citation statements)

References 60 publications

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“…1–6 weeks) [2]. Glutamate is known to be a chemotaxis-inducing factor for human neutrophils, and glutamate has demonstrated beneficial effects on both mucosal and epidermal wound healing [10, 14, 21]. …”

Section: Discussionmentioning

confidence: 99%

Functional weight-bearing mobilization after Achilles tendon rupture enhances early healing response: a single-blinded randomized controlled trial

Valkering¹,

Aufwerber

Ranuccio

et al. 2016

Knee Surg Sports Traumatol Arthrosc

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PurposeFunctional weight-bearing mobilization may improve repair of Achilles tendon rupture (ATR), but the underlying mechanisms and outcome were unknown. We hypothesized that functional weight-bearing mobilization by means of increased metabolism could improve both early and long-term healing.MethodsIn this prospective randomized controlled trial, patients with acute ATR were randomized to either direct post-operative functional weight-bearing mobilization (n = 27) in an orthosis or to non-weight-bearing (n = 29) plaster cast immobilization. During the first two post-operative weeks, 15°–30° of plantar flexion was allowed and encouraged in the functional weight-bearing mobilization group. At 2 weeks, patients in the non-weight-bearing cast immobilization group received a stiff orthosis, while the functional weight-bearing mobilization group continued with increased range of motion. At 6 weeks, all patients discontinued immobilization. At 2 weeks, healing metabolites and markers of procollagen type I (PINP) and III (PIIINP) were examined using microdialysis. At 6 and 12 months, functional outcome using heel-rise test was assessed.ResultsHealing tendons of both groups exhibited increased levels of metabolites glutamate, lactate, pyruvate, and of PIIINP (all p < 0.05). Patients in functional weight-bearing mobilization group demonstrated significantly higher concentrations of glutamate compared to the non-weight-bearing cast immobilization group (p = 0.045).The upregulated glutamate levels were significantly correlated with the concentrations of PINP (r = 0.5, p = 0.002) as well as with improved functional outcome at 6 months (r = 0.4; p = 0.014). Heel-rise tests at 6 and 12 months did not display any differences between the two groups.ConclusionsFunctional weight-bearing mobilization enhanced the early healing response of ATR. In addition, early ankle range of motion was improved without the risk of Achilles tendon elongation and without altering long-term functional outcome. The relationship between functional weight-bearing mobilization-induced upregulation of glutamate and enhanced healing suggests novel opportunities to optimize post-operative rehabilitation.Electronic supplementary materialThe online version of this article (doi:10.1007/s00167-016-4270-3) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Functional weight-bearing mobilization after Achilles tendon rupture enhances early healing response: a single-blinded randomized controlled trial

Valkering¹,

Aufwerber

Ranuccio

et al. 2016

Knee Surg Sports Traumatol Arthrosc

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“…Another example is the tryptophan metabolite kynurenate, which exerts antiinflammatory properties by inhibiting xanthine oxidase, resulting in decreased production of ROS during hypoxic stress (76). Likewise, glutamate, methionine, and threonine appear to improve colonic mucosal regeneration after insult (77). These examples provide insight into the integrated responses necessary for homeostatic regulation of tissue barrier function.…”

Section: Molecular Aspects Of Barrier Regulation In the Mucosamentioning

confidence: 99%

Oxygen metabolism and barrier regulation in the intestinal mucosa

Glover¹,

Lee²,

Colgan³

2016

Journal of Clinical Investigation

136

108

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“…Another study performed in the same model has shown that the combination of threonine, methionine and glutamate supplementation ameliorates colonic MH but not inflammatory status [99]. Although the mechanisms of action by which the three AA accelerate the colonic MH remain unclear, it can be presumed, as already discussed above, that these AA may serve as building blocks for macromolecule synthesis in the wounded mucosal area, as energy substrates for anabolic pathways, and as precursors for bioactive metabolites [100].…”

Section: Potential Role Of Dietary Protein Intake and Amino Acid Smentioning

confidence: 99%

“…In animal studies, colitis has been shown to be associated with a marked increase in the protein fractional synthesis rate in the colon [99,119,120,121]. In addition, in a mice model of colitis, an HP intake (whole milk protein), although increasing the mortality of animals when compared to an isoenergetic NP diet, displayed beneficial effects on the surviving animals during the colitis remission phase [45].…”

Section: Potential Role Of Dietary Protein Intake and Amino Acid Smentioning

confidence: 99%

Dietary Protein and Amino Acid Supplementation in Inflammatory Bowel Disease Course: What Impact on the Colonic Mucosa?

et al. 2017

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Inflammatory bowel diseases (IBD), after disease onset, typically progress in two cyclically repeated phases, namely inflammatory flare and remission, with possible nutritional status impairment. Some evidence, either from epidemiological, clinical, and experimental studies indicate that the quantity and the quality of dietary protein consumption and amino acid supplementation may differently influence the IBD course according to the disease phases. For instance, although the dietary protein needs for mucosal healing after an inflammatory episode remain undetermined, there is evidence that amino acids derived from dietary proteins display beneficial effects on this process, serving as building blocks for macromolecule synthesis in the wounded mucosal area, energy substrates, and/or precursors of bioactive metabolites. However, an excessive amount of dietary proteins may result in an increased intestinal production of potentially deleterious bacterial metabolites. This could possibly affect epithelial repair as several of these bacterial metabolites are known to inhibit colonic epithelial cell respiration, cell proliferation, and/or to affect barrier function. In this review, we present the available evidence about the impact of the amount of dietary proteins and supplementary amino acids on IBD onset and progression, with a focus on the effects reported in the colon.

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Beneficial Effects of an Amino Acid Mixture on Colonic Mucosal Healing in Rats

Abstract: Amino acid supplementation ameliorates colonic MH but not mucosal inflammatory status. Our data sustain the use of adjuvant dietary intervention on initiated intestinal MH.

Cited by 22 publications

References 60 publications

Functional weight-bearing mobilization after Achilles tendon rupture enhances early healing response: a single-blinded randomized controlled trial

Functional weight-bearing mobilization after Achilles tendon rupture enhances early healing response: a single-blinded randomized controlled trial

Oxygen metabolism and barrier regulation in the intestinal mucosa

Dietary Protein and Amino Acid Supplementation in Inflammatory Bowel Disease Course: What Impact on the Colonic Mucosa?

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