Beneficial effects of Androctonus australis hector venom and its non-toxic fraction in the restoration of early hepatocyte-carcinogenesis induced by FB1 mycotoxin: Involvement of oxidative biomarkers

Bekkari, Nadjia; Laraba-Djebari, Fatima

doi:10.1016/j.yexmp.2015.06.022

Cited by 13 publications

(4 citation statements)

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“…It was reported that reactive oxygen species (ROS) are probable mediators of cytotoxicity and their role in cancer development, metastasis, progression, and survival is well documented (Cerutti 1994 ; Sur and Ganguly 1994 ; Iqbal and Okada 2003 ; Goswami et al 2008 ; Tong et al 2015 ). Interestingly, similar results were obtained with the venom of A. australis which significantly decreased level of oxidative stress biomarkers in mice hepatic tumor (Bekkari and Laraba-Djebari 2015 ).…”

Section: Discussionsupporting

confidence: 79%

“…These results were further supported by liver histopathology. Recently, Bekkari and Laraba-Djebari ( 2015 ) reported the beneficial effects of A. australis venom which partially restored the hepatic alteration induced by the carcinogenic agent of fumonisin (FB1 mycotoxin). Moreover, reducing extent of oxidative stress may explain how A. amoreuxi venom protected liver of treated animals from damage induced by tumor.…”

Section: Discussionmentioning

confidence: 99%

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In vitro and in vivo antitumor effects of the Egyptian scorpion Androctonus amoreuxi venom in an Ehrlich ascites tumor model

et al. 2016

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Scorpion venom is a highly complex mixture of about 100–700 different components, where peptides are the major constituents with various biological and pharmacological properties including anticancer activities. In this study, anticancer efficacy of the venom of the Egyptian scorpion Androctonus amoreuxi has been evaluated. In vitro, the human breast cancer MCF-7 cell line was treated with the venom and the IC50 was estimated. In vivo studies, Ehrlich ascites carcinoma (EAC) cells were inoculated into CD-1 mice intraperitoneally to form liquid tumor or subcutaneously to form solid tumor and then treated with intraperitoneal injection with venom (0.22 mg/kg) every other day. The total tumor cells in the ascitic fluid and the size of the solid tumor were assessed after 14 and 30 days, respectively. In addition, the mean survival time (MST), body weight, tumor volume, PCV, viability of tumor cells, CBC, AST, ALP, creatinine, oxidative stress biomarkers (GSH, MDA, PCC), tumor marker Ki67, growth factor VEGF and caspase-3 were measured in normal control, EAC control and venom-treated groups (n = 6). Treatment with venom induced anti-tumor effects against liquid and in solid tumors as indicated by a significant (P < 0.05) reduction in tumor volume/size, count of viable EAC cells, expression of Ki67 and VEGF as well as by remarkable increases in MST and caspase-3 expression as compared to non-treated group. Interestingly, the venom restored the altered hematological and biochemical parameters of tumor-bearing animals and significantly increased their life span. These data indicate to (1) the cytotoxic potential effects of A. amoreuxi on tumor cells via anti-proliferative, apoptotic and anti-angiogenic activities; (2) opening a new avenue for further studies on the anti-cancer effects of this agent.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

In vitro and in vivo antitumor effects of the Egyptian scorpion Androctonus amoreuxi venom in an Ehrlich ascites tumor model

et al. 2016

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“…In a recent study, our research team demonstrated the ability of Aah venom and its non-toxic fraction 1 (F1) to inhibit proliferation of early stage hepatocarcinoma induced in vivo by Fumonisin B1 mycotoxin [ 23 ]. In the same context, the present study was carried out to investigate the antiproliferative and cytotoxic induction capacity of Aah crude venom and its toxic fractions (FtoxG-50 and F3) on cancer cells in vitro.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic activity of Androctonus australis hector venom and its toxic fractions on human lung cancer cell line

Béchohra

Laraba-Djebari

Hammoudi-Triki

2016

J Venom Anim Toxins Incl Trop Dis

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BackgroundSeveral studies have showed that animal venoms are a source of bioactive compounds that may inhibit the growth of cancer cells, which makes them useful agents for therapeutic applications. Recently, it was established that venom toxins from scorpions induced cytotoxic, antiproliferative and apoptogenic effects on cancer cells. Therefore, the present study aims to investigate the cytotoxic activity of Androctonus australis hector (Aah) scorpion venom and its toxic fractions (FtoxG-50 and F3) on NCI-H358 human lung cancer cells.MethodsThe cytotoxic and antiproliferative activities were estimated using MTT assay, lactate dehydrogenase release and clonogenic assays. Apoptosis was evaluated by Hoechst 33258 staining, DNA fragmentation assay and caspase-3 activity. Oxidative stress was analyzed by reactive oxygen species, nitric oxide, malondialdehyde and protein carbonyl levels along with assessment of antioxidant status. In addition, alteration of mitochondrial membrane potential was analyzed by JC1 fluorescent dye.ResultsThe present findings showed that F3 fraction was more cytotoxic towards NCI-H358 lung cancer cells with an IC50 of 27.05 ± 0.70 μg/mL than venom alone (396.60 ± 1.33 μg/mL) and its toxic fraction FtoxG-50 (45.86 ± 0.91 μg/mL). Nevertheless, F3 fraction was not cytotoxic at these concentrations on normal human lung fibroblast MRC-5 cells. Inhibition of NCI-H358 cell proliferation after F3 fraction exposure occurred mainly by apoptosis as evidenced by damaged nuclei, significant DNA fragmentation level and caspase-3 activation in a dose dependent manner. Moreover, F3 fraction enhanced oxidative and nitrosative stress biomarkers and dissipated mitochondrial membrane potential in lung cancer cells along with significant depletion in cellular enzymatic and non-enzymatic antioxidants. Further, the apoptosis induced by F3 fraction was markedly prevented by the antioxidant N-acetylcysteine (NAC) suggesting the potential mechanism of oxidative stress.ConclusionThese findings suggest that F3 fraction could induce apoptosis in lung cancer cells through involvement of oxidative stress and mitochondrial dysfunction. Hence, these properties make F3 fraction a promising candidate for development of new anticancer agents.

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“…This could be due to the fact that this venom has a protective property against liver injury (supported by liver histopathology). Interestingly, Nadjia and Fatima [13] previously reported that A. australis venom treatment has a beneficial effect on hepatic toxicity induced by fumonisin. Furthermore, SOD and CAT activities were decreased in EACbearing mice as a result of tumor propagation.…”

Section: Discussionmentioning

confidence: 99%

<i>In vivo</i> anti-tumor effect of Egyptian scorpion <i>Leiurus quinquestriatus</i> venom in Ehrlich ascites carcinomabearing mice

Salama,

El-Naggar,

ALRashdi

2023

Trop. J. Pharm Res

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Purpose: To investigate the anti-tumor efficacy of scorpion Leiurus quinquestriatus venom (LQV) in Ehrlich ascites carcinoma (EAC) mouse model.Methods: Mice were divided into 4 groups (n = 8), with Group 1 as negative control. Groups 2 to 4 mice were inoculated with EAC cells (1 x 106/mouse) intraperitoneally (ip). Group 2 mice were untreated while groups 3 and 4 mice were injected with 2 mg/kg of cisplatin (Cis) and 0.025 mg/kg of LQV ip, respectively, for 7 days. Tumor profile, as well as hematological and biochemical analyses were carried out.Results: Tumor volume and tumor cell counts decreased significantly (p < 0.05) following LQV treatment. Also, LQV potentiated necrosis, apoptosis and arrested tumor cells in the G0 and S-phases. Furthermore, it upregulated apoptotic-related gene (Bax and caspase-3) expressions and down-regulated anti-apoptotic gene (bcl-2) expression in EAC cells (p < 0.05).Conclusion: LQV has potential anti-tumor activity against EAC cells in mouse models. Therefore, it should be further investigated in vivo as an anti-tumor agent.

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Beneficial effects of Androctonus australis hector venom and its non-toxic fraction in the restoration of early hepatocyte-carcinogenesis induced by FB1 mycotoxin: Involvement of oxidative biomarkers

Cited by 13 publications

References 48 publications

In vitro and in vivo antitumor effects of the Egyptian scorpion Androctonus amoreuxi venom in an Ehrlich ascites tumor model

In vitro and in vivo antitumor effects of the Egyptian scorpion Androctonus amoreuxi venom in an Ehrlich ascites tumor model

Cytotoxic activity of Androctonus australis hector venom and its toxic fractions on human lung cancer cell line

<i>In vivo</i> anti-tumor effect of Egyptian scorpion <i>Leiurus quinquestriatus</i> venom in Ehrlich ascites carcinomabearing mice

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