Beneficial effects of carrot pectin against lead intoxication in Wistar rats

Abstract

The aim of the present study was to investigate the beneficial action, in vivo, of pectin against subacute lead acetate (350 mg/l) intoxication. The adverse effects of lead on the haematological disturbances that concerned, more precisely, the decrease of red blood corpuscle life duration and on the appearance of ever granulated basophilic haematites by inhibiting an enzyme responsible for haeme synthesis have been demonstrated after 1 month of oral lead administration to female Wistar rats. Also, this caused an elevation of the blood lead level as compared with the control group. The introduction of carrot pectin to a level of 3% in the feeding of intoxicated rats has shown a chelating and correcting effect on haematological disturbances caused by lead toxicity, which is reflected by a significant decrease (P<0.05) of blood lead (from 117 to 65 to 19 μg/l), zinc protoporphyrine (portophyrine-zinc from 7.7 to 5.1 to 3.5 μg/g of Hb), increase in haemoglobin to 27% (from 5.09 to 6.05 to 7.79%) and iron to 8% (from 1.34 to 0.9 to 0.5%) of the treated rats by pectin as compared with the untreated groups. Differences in blood lead were significant between the control diet and the addition of pectin therefore suggesting that pectin fibre ingestion in diets decreases the risk of lead poisoning.

Pectin Modulates Hemato-Biochemical Parameter, Histopathology, Oxidative Stress Biomarkers, Cytokines and Expression of Hepcidn Gene in Lead Intoxicated Rats

“…While in subgroup 3B (Figure 4f, g) hyperplasia of megakaryocytes and restoring of normal histological structure of bone marrow was observed and this explained the anemic picture improvement that appeared in blood indices of rats co-treated with lead acetate and pectin. Lead toxicity in subgroup 3A causing abnormal red blood cells which clear in blood smear (field stain) shown in figure 5 B1-B4) development of basophilic granules, hypo chromatic, tear drops and heamolyzied RBCs, which are characteristics of anemia caused by lead poisoning (43,44), as previously noted (45). While in subgroup 3B, figure (5C) showed, only hypo chromatic RBCs due to protective effect of pectin.…”

Effect of Carrot-Juice on Exopolisaccharides and β-D Galactosidase Activity in Yogurt

“…Carrot (DaucuscarrotaLinn) is one of vegetables consumed either fresh or as carrot juice as the source of β-carotenes and vitamins B1, B2 and B3. These nutrients have a role in growth and repair of tissue, help the body to prevent microorganism infection and intoxicationactivity (Ouldali, 2011;Amany et al, 2012). Vitamins and β-carotenes deficiency is one of the nutritional problems, primarily affecting infants, children and women of childbearing age.…”