Beneficial effects of crocin against H2O2-induced oxidative stress on bovine sperm motility and viability

Cellular Basis and Optimization of Cryopreservation Survival of Bull Sperm = Base celular y optimización de la supervivencia de la criopreservación de esperma de toro

“…Our study is the first on the supplementing of semen freezing extender with crocin in bull, while there are two other studies about the supplementation of the thawing medium with crocin in bull (Sapanidou et al, 2015;Tsantarliotou et al, 2016). These studies obtained good results in terms of sperm motility, viability, and acrosomal integrity.…”

“…The supplementation of bull semen with 1 mM of crocin post-thawing improved the motility, viability, and acrosomal integrity of sperm, reducing the ROS levels and lowering lipid peroxidation (Sapanidou et al, 2015;Tsantarliotou et al, 2016). Cyclophosphamide-treated Mice that received an intraperitoneal dose of crocin showed improved sperm quality (Bakhtiary et al, 2014).…”

“…Thus, we tested the supplementation of the freezing extender with trehalose (a cryoprotectant with some antioxidant properties), reduced glutathione (GSH), curcumin, and crocin. These substances may contribute to removing ROS or protecting the spermatozoa against oxidative stress, hence increasing the post-thawing semen quality (Hu et al, 2010;Tsantarliotou et al, 2016;Shah et al, 2017a;Shah et al, 2017b). Whereas there are some studies about using these antioxidants with bull spermatozoa, there is a lack of reports related to their use for supplementing the BIOXcell extender.…”

“…Sapanidou et al (2015) showed that incubation of frozen-thawed bull sperm with crocin (1 mM) for 120 and 240 minutes led to enhance the semen quality and in vitro fertility of sperm. The same previous research group (Tsantarliotou et al, 2016) did another experiment by incubation of the frozen-thawed bull sperm with crocin (1 mM) for 30 min and 60 minutes in a media contain H 2 O 2 to induce oxidative stress to sperm. They found an improvement in the sperm motility and physiology parameters after the incubation, comparison to the control group.…”