Beneficial Effects of Ethanolic Leaf Extract of Coriandrum Sativum on Metanil Yellow Induced Alteration Inactivity of Catalase and Level of Lipid Peroxidation in Hercine Cardiac Tissue in Vitro

Abstract

Objective: The primary aim of the study was to find out a suitable effective dose of metanil yellow that can cause alterations of lipid peroxidation and catalase activity in the heart in vitro and to determine the effectiveness of graded doses of Coriandrum sativum as an antioxidant in preventing lipid peroxidative damage to cardiac tissue and back up catalase activity. Methods:Metanil yellow at different doses (10, 25, 35, 50 µl in phosphate buffer) were applied on isolated hircine heart homogenate (10%) in in vitro followed by estimation of lipid peroxidation and catalase activity after completion of the incubation period. Another set of the experiment included incubation of the heart homogenates with graded dilutions of ethanolic leaf extracts of Coriandrum sativum (CsEth) at concentrations of 10, 20, 30, 40 µl in phosphate buffer medium followed by quantification of the same biochemical parameters as above. This was followed by coadministration of the selected effective dose of metanil yellow (35 µl) and ethanolic leaf extract of Coriandrum sativum (30 µl) on heart homogenate to track the variation in lipid peroxidation and catalase activity in heart homogenate in vitro.

Ações Toxicológicas Dos Aditivos Alimentares No Sistema Cardiovascular

“…A ingestão do amarelo metanil pode causar modificações no padrão metabólico, promovendo a produção de radicais livres que levam ao estresse oxidativo (AL-MALKI; SAYED, 2013). O amarelo metanil foi responsável por promover uma redução significativa da ação da enzima antioxidante catalase no tecido cardíaco e uma consequente elevação da peroxidação lipídica (LPO) (DOME et al, 2017) (Figura 4). Dessa forma, os autores sugeriram que tal adulterante tem potencial de causar lesão cardíaca, sendo classificado como um forte indutor de infarto do miocárdio mediado pelo estresse oxidativo.…”

“…Dessa forma, os autores sugeriram que tal adulterante tem potencial de causar lesão cardíaca, sendo classificado como um forte indutor de infarto do miocárdio mediado pelo estresse oxidativo. Outros autores ratificam o estudo de Dome et al (2017) ao revelarem que a exposição ao amarelo metanil de coração isolado de animais causa uma indução de radicais livres no órgão, ressaltando também uma atenuação significativa na atividade da catalase, com excesso de H 2 O 2 e superóxidos (HAZRA et al, 2016). Ademais, Priscilla e Prince (2009) apontam também a peroxidação lipídica como mecanismo de formação de radicais livres, causando alterações oxidativas dos ácidos graxos poli-insaturados da membrana miocárdica, sendo essa uma das formas de progressão do infarto do miocárdio.…”

Neuropharmacological and gastrointestinal evaluation of coloring agent metanil yellow used in food and beverages

“…Metanil yellow increases defecation rate slightly in both castor and MgSO 4 induced diarrheal test. Metanil yellow consumed through food enters digestive system and causes gastro-intestinal toxicity, hepatotoxicity and damages the intestine (Nagaraja & Desiraju, 1993;Dome et al, 2017).…”

Experimentally designed chemometric models for the assay of toxic adulterants in turmeric powder

“…9 The electron-withdrawing character of the azo group in the synthetic dyes sometimes develops an electron deciency and is reduced to carcinogenic amino compounds. 10 Long-term consumption of metanil yellow (MY) (sodium 3-[4-anilinophenylazo]benzene sulfonate) causes severe damage to the heart and nervous tissues, 11,12 degenerative changes in the lining of the stomach, kidneys and liver, 13 as well as adversely affects the ovaries and testes. 14,15 Acid orange 7 (AO) (sodium 4-[(2E)-2-(2-oxo-naphthalene-1ylidene)hydrazinyl]benzene sulfonate) and lead chromate are deliberately added to adulterate turmeric powder.…”