Beneficial Effects of Intraperitoneally Administered Alpha-Tocopheryl Acetate on the Levels of Lipid Peroxide and Activity of Glutathione Peroxidase and Superoxide Dismutase in Skin, Blood and Liver of Thermally Injured Guinea Pigs

Abstract

The aim of this study was to investigate the effects of intraperitoneal α-tocopheryl acetate administration on concentration of lipid peroxide (as malonyldialdehyde, MDA), reduced glutathione (GSH), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in plasma, red blood cells (RBC), skin and liver of thermally injured guinea pigs. MDA levels in the plasma, skin and liver were increased at the 15th min after the thermal injury compared to the control group. However, they were significantly lower in the α-tocopheryl-acetate-treated groups than both control and thermally injured groups from the 15th min to the 6th day. SOD activity of the skin and RBC and vitamin E levels in the plasma were significantly decreased between the 15th min and 6th day and then both parameters, i.e. SOD and vitamin E, were significantly increased by α-tocopheryl acetate administration. GSH-Px activity in the skin was also significantly decreased in groups from the 15th min to the 6th day, whereas it remained unchanged by the α-tocopheryl acetate administration. On the other hand, GSH-Px activity and GSH levels in the RBC remained the same during both thermal injury and α-tocopheryl acetate administration. In conclusion, we observed that the intraperitoneal administration of α-tocopheryl acetate protects the skin against thermal injury, which suggests that it may be due to the upregulation of enzymatic antioxidants.

Topically applied metal chelator reduces thermal injury progression in a rat model of brass comb burn

“…Some of these studies are focused on lipid peroxidation inhibition [31], platelet activating factor antagonism [32], xanthine-oxidase inhibition [8, 33, 34], and treatment with hydroxyl radical scavengers and anti-oxidant enzymes themselves or the enzyme stimulator (melatonin) [33, 35, 36]. Meanwhile, some other studies reported treatment with rosiglitazone, a agonist of PPAR-γ (peroxisome proliferator-activated receptor gamma) [37], pretreatment of curcumin with antioxidant and antiapoptotic properties [38], and supplement of esterified glutathione (GSH) [39], vitamin C [40, 41] or α-tocopherol with vitamin E activity [42]. Our current study further focused on therapeutic intervention aiming to block the formation of free radicals with a metal chelator.…”